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피브로넥틴과 펠렛배양이 골막유래 전구세포의 인슐린 생산 세포로의 분화에 미치는 영향
박부영 ( Bu Young Park ),김종일 ( Jong Il Kim ),전형진 ( Hyung Jin Jeon ),최성훈 ( Sung Hun Choi ),이창우 ( Chang Woo Lee ),임상민 ( Sang Min Lim ),김동일 ( Dong Il Kim ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4
Type 1 diabetes is caused by a progressive destruction of insulin-producing cells(IPCs) by an autoimmune disease. Islet transplantation is considered as the most effective treatment for type 1 diabetes, but it is hindered by limited supply of islets. Mesenchymal stem cells(MSCs) can be isolated from several tissues and used in the differentiation into mesodermal cell types. Periosteum-derived progenitor cells(PDPCs), which have similar characteristics with MSCs, were easily isolated with high growth potency. Using a three-step differentiation protocol, PDPCs could be differentiated into IPCs. IPCs derived from PDPCs were evaluated by using microscope, RT-PCR and immunostaining. When three-dimensional suspension cultures were performed with the addition of fibronectin and various extracellular matrix(ECM), it was clear that PDPCs could differentiate into IPCs. Under optimized culture conditions, improved differentiation of PDPCs into IPCs was possible.
연골 특이적 ECM 및 영양세포가 골막 유래 전구세포의 생존도와 GAG 생성량에 미치는 영향
김종일 ( Jong Il Kim ),박부영 ( Bu Young Park ),임동균 ( Dong Kyoon Lim ),최성훈 ( Sung Hun Choi ),김동일 ( Dong Il Kim ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4
Periosteum-derived progenitor cells(PDPCs) have a capacity to differentiate into a number of connective tissue lineage. They are an attractive source of chondrocyte-progenitor cells. When these cells are cultured in a threedimensional format in the presence of transforming growth factor-b3(TGF-b3), they showed characteristic morphological changes with the deposition of cartilaginous extracellular matrix(ECM). In this study, effects of various cartilage-specific ECMs or feeder cells on the chondrogenesis of PDPCs were investigated. Application of this system resulted in a more functional format and enhanced the cell viability than the chondrogenesis without cartilage-specific ECMs or feeder cells. Differentiation was found to be dependent on immunophenotypes and was interrelated with passage number. FACS analysis on CD29, CD44, CD105 by serial passage numbers of PDPCs provided an optimal seeding time for chondrogenesis. Addition of chondroitin 6-sulfate, known as cartilage-specific ECM, in induction media enhanced chondrogenesis, as indicated by the synthesis of glycosaminoglycan(GAG). When chondrocytes were used as feeder cells, the cell viability was increased during chondrogenesis.
중간엽 줄기세포의 연골세포로의 분화능에 (-)-에피갈로카테킨 갈레이트가 미치는 영향
송지혜 ( Ji Hae Song ),박부영 ( Bu Young Park ),김종일 ( Jong Il Kim ),최성훈 ( Sung Hun Choi ),김동일 ( Dong Il Kim ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.1
The cell therapy using tissue-engineered cartilage is essential because the surgical operation for the repair of defected cartilage is not sufficient. Mesenchymal stem cells(MSCs) are an ideal candidate for use in cell-based therapy because of their self-renewal ability and differentiation capability into specific cells such as chondrocytes. Especially, human umbilical cord blood(UCB) is an attractive cell source because it can be obtained easily and the process is noninvasive and painless. However, the life span of MSCs are limited and the accumulation of intracellular oxidative damage by aging causes the loss of differentiation capacity during ex vivo expansion. (-)-Epigallocatechin gallate(EGCG) is known as an anti-oxidant agent which has a role as a free radical scavenger. It can improve long-term cell viability. Therefore, differentiation capacity can be maintained. In this study, effects of EGCG on chondrogenic potential of UCB-MSCs were investigated. The proliferation and differentiation capacity of UCBMSCs was characterized during expansion. Optimal concentration of EGCG was decided. Addition of EGCG in the media increased the cell viability during chondrogenic induction. Glycosaminoglycan(GAG), a cartilage-specific extracellular matrix(ECM), was also increased. It was confirmed that EGCG could improve the chondrogenic potential of UCB-MSCs by reducing oxidative stress. These results can contribute to the repair of damaged articular cartilage using UCB-MSCs.