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정대성,민두식,김병로 ( Tae Seong Cheong,Du Sik Min,Byoung Ro Kim ) 한국산림과학회 1985 한국산림과학회지 Vol.71 No.1
In this study, the vegetation of Pinus densiflora S. et Z., Pinus regida Miller, Pinus koraiensis S. et Z., and Larix kaempferi Satgent (major conifers) stands planted in the Chungcheong-province was investigated to obtain the fundamental informations for the improvement of coniferous barks utilization. The results may be summarized as follows; 1) Barks are much richer in. quantity of extractives and lignin than the corresponding wood. Alcohol-benzene extractives of Pinus koraiensis barks are the highest among others. Pentosan contents are lower in the bark than in the wood, but pentosan contents of the Larix kaempferi bark is the highest among others. 2) Barks are acid in nature, and PH values of barks varying from 3.5 to 4.1 are lower than that of wood. The ash contents of barks are greater than the corresponding wood. 3) A bark comprises from 13.9 to 19.8 percents of a typical log by volume. The calorific values are higher in the barks than in the wood, and calorific values of Pinus koraiensis barks are the highest among others.
Cellulase 에 의한 목재당화에 (木材糖化) 관한 연구 - (Ⅰ) 기질 (基質) 처리의 효과 -
정대성,민두식 ( Tae Seong Cheong,Du Sik Min ) 한국산림과학회 1978 한국산림과학회지 Vol.38 No.1
In this study, enzymatic hydrolysis of the holocellulose from Alnus hirsuta (Spach) Rupr. (8-14 yr`s) was investigated using crude cellulase preparations of Trichoderma viride Pers. ex. Fr. SANK 16374. And conducted on the optimum condition of the treated substrate for saccharification. A strain of Trichoderma viride Pers. ex. Fr. SANK 16374 was found to be highly efficient for the cellulase productivity, especially in the submerged culture process. The culture medium used in this experiment was prepared from an extract of wheat bran consisting also of KH₂ PO₄ 10, (NH₄)₂ SO₄ 3, NaNO₃ 3, and MgSO₄7H₂O 0.5g/1. Cellulose powder (Toyo filter paper, 60 mesh) was found to be an importent factar for inducing the cellulase formation. And the cellulase produced in the culture fluid was salted out quantitatively by the use of ammonium sulfate (Fig. 1) Reducing sugar was determined by the Dinitrosalicylic acid (DNS) method, using reagents prepared according to the method of Sumner (1925). The results obtained were summerized as follows; 1. The method of delignification were treated by the Peracetic acid (PA) method, according to the method of Toyama (1970). The yield of holocellulose were decreased in accordance with increasing concentration of Peracetic acid solution; delignification of Alnus hirsuta Rupr. with 20% Peracetic acid was satisfied for 48 hours and 40%∼60% peracetic acid was satisfied for 24 hrs: 2. The substrate (holocellulose) was changed easely into fine powder with enzymatic hydrolysis and cellulase exhibits optimum activity on the reducing sugar formation from substrate at the range of 60-100 mesh. 3. The reducing sugar formation increased in accordance with increasing dry temperature on holocellulose substrate was found to be 190±5℃. 4. The optimal heat treated time of holocellulose substrate was found to be 45 min. for the reducing sugar formation showed the best products. The reducing sugar formation did not show statisticaly significent diflerences at 5% levels by heat treated time for 45 min. and 60 min.
鄭大成,閔斗植,李學州 충북대학교 농업과학기술연구소 1988 農業科學硏究 Vol.6 No.1
This study was Conducted to obtain some fundamental information on the chemical components of leguminous foliage comprising carbohydrates, lignin, extractives, composition of essential elements, pH and protein. Test materials used in this study were the foliage of seven leguminous species. (see, Table 1) Chemical analyses, of organic and inorganic components were performed by TAPPI standard and A.O.A.C. methods. 1.The extractive contents, carbohydrates and lignin of foliage are that the cold water extractives are from 17.7 to 25.37, hot water from 21.2 to 29.7%, 1% NaOH from 41.7 to 65.8%, alcohol : benzen from 6.4 to 12.4%, holocellulose contents from 37.1 to 57.7%, pentosan from 13.6 to 18.8% and lignin from 25.4 to 41.6%. 2.The essential elements of foliage are that the crude carbon contents are from 28.8 to 47.5%, N. from 1.05 to 2.79%, Ca. from 0.56 to 1.47%, Mg. from 0.22 to 0.37%, K. from 1.08 to 4.76%, P. from 0.18 to 0.38%, Cl. from 0.04 to 0.39%, Si. from 0.40 to 2.08% and ash from 4.92 to 8.17%. 3.pH values of the leguminous foliage from 5.19 to 6.82. And crude protein contents are from 10.6 to 18.8% of it.