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치주 질환 심도와 치은열구액내 β-glucuronidase에 관한 연구
문진균,김평환,김형섭 全北大學校 齒醫學硏究所 1990 전북치대논문집 Vol.8 No.1
Because traditional clinical measures of human periodontal disease cannot be detected or predicted the occurance of clinical attachment loss, new biochemical workers were developed in diagnosis of periodontal disease. β-glucuronidase, for such a marker associated with tissue breakdown, was analysed from gingival crevicular fluid of 101 periodontal patients aged 27-62 years. The filter paper strip was inserted into the gingival sulcus of the labial surface of the maxillary left central incisor and gingival crevicular fluid colleted. By the modification of the method of Dingle (9172), β-glucuronidase was assayed spectrophotometrically from supernatant enzyme preparation, and compared with clinical parameters such as pocket depth or sulcus bleeding index. 1. As the grade of sulcus bleeding index was increased, the periotron units and β-glucuronidase activity were increased significant(P<0.05). 2. Correlation between SBI and β-glucuronidase, SBI and periotron units, periotron units and β-glucuronidase were relatively low. Those correlation between periotron units and β-glucuronidase was the highest (γ=0.34). 3. As the grade or pocket depth was increased, periotron units and β-glucuronidase were not increased significantly(P>0.05).
Substance P가 T 임파구의 세포증식과 IL-2 생산에 미치는 영향
문진균,최병선,이석초,김형섭,Moon, Jin-Kyun,Choi, Byung-Son,Lee, Seok-Cho,Kim, Hyung-Seop 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.4
Immune responses of periodontal tissue may be regulated by products of sensory afferent nerve endings such as neuropeptides. Substance P(SP), a tachykinin neuropeptide, has been previously reported to stimulate the activities of T lymphocyte. Therefore, I examined the role of SP in IL-2 production and cell proliferation by using a homogeneous line of T lymphocytes(Jurkat and HuT78). Cell proliferation rate was determined by [$^3H$]-thymidine incorporation test, and IL-2 was quantitated by the growth rate of CD4+ IL-2-dependent T lymphocyte line CTLL-2. SP stimulated cell proliferation of T lymphocytes at the concentration of $10^{-12}$ and $10^{-8}$M in a biphasic bell-shape dose-dependent manner. However, SP alone did not induce IL-2 release at the concentration range of $10^{-6}$ to $10^{-14}$M. The upregulation of IL-2 release was observed when $10^{-12}$M SP was applied together with mitogens such as Con A or PHA+PMA on T cell lines, especially on Jurkat. Con A or PHA+PMA demonstrated to increase the rate of cell proliferation of Jurkat, which had shown to produce much amount of IL-2 indicating that mitogen-induced cell proliferation might be partially influenced by released IL-2. It was concluded that regulatory effects of SP on the immune/inflammatory response could be mediated through the costimulatory upregulation of IL-2 production and increase of cell proliferation of T lymphocyte.
Tetracycline Hydrochloride 적용방법에 따른 치근면 표면 형태 변화의 연구
류성훈,문진균,김형섭,Rew, Seong-Hun,Moon, Jin-Kyun,Kim, Hyung-Seop 대한치주과학회 1995 Journal of Periodontal & Implant Science Vol.25 No.1
The purpose of this was to evaluate the in vitro effects of different application modes of tetracycline HCI on dentin root surfaces. The study group was comprised of 30 teeth with advanced periodontal disease extracted before the start of periodontal therapy. The diseased root surface was vigorously root planed to expose dentin. The teeth were sectioned and cotton pellets. soaked in a saturated solution of tetracycline HCI, were either "placed" or "burnished" on the prepared root surface for 3, 5 min. respectively. Control surfaces were similarly treated with root planing only. Following the various treatments, teeth were fixed. dehydrated, critical point dried, and coated for scanning electron microscopic(SEM) evaluation. The surfaces of tetracycline HCI treated specimens considerably differed from specimens treated with root planing only. Results demonstrated that root-planed, no-tetracycline HCI specimens had amorphous, irregular surface which corresponded to a smear layer. Tetracycline HCI specimens exhibited collagen fibers and dentinal tubules exposed by the removal of the smear layer. Although some differences were seen in surface structures among some specimens. "burnished" application causes more extensive change than "placed" application, and application time of the tetracycline HCl did not seem to have any major impact on the surface morphology.
임종득,문진균,김형섭,Lim, Jong-Deuk,Moon, Jin-Kyun,Kim, Hyung-Seop 대한치주과학회 1994 Journal of Periodontal & Implant Science Vol.24 No.1
This study was undertaken to examine the protein content of GCF and serum from noraml population in order to standardize the sample loading on SDS/PAGE gels. The resulats were as follows ; 1. The protein concentration of serum was not different between normal group and diseased group. 2. In GCF, the bands of lower molecular weight than albumin were heavily stained, but in serum, the protein bands of higher molecalar weight were found. 3. The profile of protein in normal GCF was characterized by heavily staining bands at 77, 66, 55, 26 KDa corresponding to the positions of transferrin, albumin, heavy and light chains of Ig G. Also 47, 37 KDa nonplasma proteins were found.