피부에 전이된 소세포성 폐암 2예

류지호 ( Ji Ho Ryou ),전범진 ( Bum Jin Juhn ),이무형 ( Mu Hyoung Lee ) 대한피부과학회 1998 대한피부과학회지 Vol.36 No.2

The frequency of metastasis to the skin from lung cancer is 1-12%. The incidence of cutaneous metastasis was high in patients with large-cell lung cancer, whereas squamous cell and small-cell lung cancer showed the least tendency to extend to cutaneous sites. Adenocarcinoma was intermediate in the tendency to metastasize in the skin. Histological findings of small-cell lung cancer show solid, tumor cell nests composed of neoplastic cells with large, round nuclei. So, it is often difficult to distinguish small-cell lung cancer from other poorly differentiated small-cell tumors, such as Merkel cell carcinoma, metastatic carcinoid, lymphoma, etc. We report here two cases of skin metastasis from small-cell lung cancer. Case one was a 74-year-old man presenting with a single, round, dome-shaped nodule on the alar nasi which had been present for 1 month. The second case was a 73-year-old man who had found three, non-tender, freely movable subcutaneous nodules on the abdomen 3 days before. Both cases were diagnosed as small-cell lung cancer. On histopathological examination of the skin lesions, we could observe that small round to oval cells with dark staining nuclei and scant cytoplasms were arranged in anastomosing trabecular streams through the dermis, separated by strands of connective tissue in both cases. In the first case, the tumor cells were positive to cytokeratin and NSE, negative to neurofilament antiagen and LCA. (Korean J Dermatol 1998;36(2): 308-312)

과립세포종 ( Granular cell tumor )

류지호 ( Ji Ho Ryou ),최재영 ( Jae Young Choi ),이무형 ( Mu Hyoung Lee ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.5

Granular cell tumors were originally described in 1926 by Abrikossoff as myoblastic myomas. They usually occur as solitary tumors but can be multiple in about 10% of cases. They have a predilection for the head, neck and tongue, but also occur in many other organs. Authors experienced a 37 year-old male patient with a granular cell tumor which exhibited the distinctive histopathologic appearance on light microscopy and electromicroscopy. Also, granular cells were S-100 protein, vimentin and CD68 immunoreactive, but desmin and GFAP showed negative immunostaining. This finding suggests a neural origin for grenular cell tumors. (Kor J Dermatol 1996;34~(5): 861-864)

고정약진의 임상 및 병리조직학적 고찰

류지호 ( Ji Ho Ryou ),김진환 ( Jin Hwan Kim ),이무형 ( Mu Hyoung Lee ) 대한피부과학회 1998 대한피부과학회지 Vol.36 No.1

Background: Fixed drug eruptions(FDE) are a cutaneous reaction characterized by one or more circumscribed lesions that recur at the same site in response to a given medication. Objective: The purpose of this study was to find the clinical and histopathological characteristics of FDE and to compare early FDE with late FDE histopathologically. Method: We clinically investigated 54 cases of FDE that visited the department of dermatology at the Kyunghee medical center from January 1993 to December 1996. Among them, 31 patients had skin biopsies and were evaluated histopathologically on the basis of duration. Results .' The results were summarized as follows: 1. Development of FDE did not show any difference according to sex and was evenly distributed over all the ages. 2. The latent periods of FDE were diverse in appearance from 30 minutes to 10 days, but mostly, the skin lesions erupted within 4S hours. 3. The most common skin lesions were erythematous macules. 4. Distribution of the lesions came out as solitary : 20.4%, multiple : 79.6%. S3.7% of the multiple lesions were localized to a part of body, and 16.3% were distributed over the whole body. 5. The areas in which the eruptions developed were (in descending order): upper extremity(37.0%), hand(31.5%), trunk(24.1%), face(24.1%). 6. In most cases(68.6%), the size and the number of lesions were greater in recurrente, rather than in first attacks. 7. The histopathological findings commonly showed perivascular mononuclear cell(MNC) infiltration (100%), pigmentary incontinence(77.4%), basal hydrophic degeneration(71.0%), eosinophil infiltration in dermis(61.3%), etc. 8. The epidermal histopathological findings such as spongiosis, exocytosis of MNC, basal hydrophic degeneration, keratinocyte necrosis and subepidermal vesicles could be seen more frequently in early lesions than in late ones. Conclusion : In our study, we were able to obtain meaningful results based on data from the combination of clinical and histopathological investigations. This study may give help to understand the characteristics of fixed drug eruptions and to plan future studies. (Korean J Dermatol 1998;36(1): 30-36)

사구종양의 면역조직화학적 고찰

류지호,이주희,김낙인 ( Ji Ho Ryou,Ju Hie Lee,Nack In Kim ) 대한피부과학회 1997 大韓皮膚科學會誌 Vol.35 No.6

Background: Glomus tumor is a benign lesion composed of vessels and glomocytes in varying proportions. Although the histc)logic features of glomus tumors by light microscopy are characteristic and well recognized, there is controversy as to the histogenesis and cytological characterization of the glomus cell. Objective : The aim of the present study was to verify the histopathological origin of a glornus tumor. Method: We investigated 1 cases of glomus tumors for immunohistochemical features. Results : The glomus turnor cells were negative when stained for FactorVIII - related antigen and S-100 protein. Conversely, all materials were found to be positive for actin, vimentin, NSE. Sorne exhibited an equivocal reaction for desmin and CD34, the rest were negative. Conclusion : These findings support the hypothesis that the glomus cell is transitional-between smooth mu.cle and vascular ndothelium-being essentially a modified smooth muscle cell with some endot.helial cell properties (KoreaA J D8rmatOI 1997;35(6): 1130-1135)

기미 환자의 삶의 질 평가

조태호 ( Tae Ho Cho ),홍석범 ( Seok Beom Hong ),류지호 ( Ji Ho Ryou ),이무형 ( Mu Hyoung Lee ) 대한피부과학회 2007 대한피부과학회지 Vol.45 No.3

Background: Melasma is a common pigmentary disorder of facial skin. It can have significant emotional, social, and psychological effects. But little is known about the impact of melasma on the quality of life (QOL) in Korean patients. Objective: The purpose of this study was to investigate the quality of life in patients with melasma. Methods: A total of 100 patients with melasma were enrolled and interviewed against the Melasma Quality of Life scale (MELASQOL), which was recently developed by Balkrishnan et al. The patients were also evaluated by the investigator using the Melasma Area and Severity Index (MASI). Results: The average score of MELASQOL was 41.2. A high score meant that melasma had a great effect on quality of life. It was high in patients with high MASI scores (p<0.01), young patients (p<0.01), and short duration of melasma (p<0.05). It was also high in those who had a high education level (p<0.05), or a previous treatment history (p<0.01). However, presence or absence of other skin diseases or preceding causes made no significant difference. Conclusion: This study shows that melasma has significant impact on the QOL of Korean melasma patients. Therefore, in addition to treating melasma, clinicians need to consider the psychosocial aspects of improving QOL in order to give patients optimal satisfaction. (Korean J Dermatol 2007;45(3):232~236)

증례 : 원발성 본태성 뇌회상 피부 1 예 ( Case Report : A Case of Primary Essential Cutis Verticis Gyrata )

최재영(Jae Young Choi),류지호(Ji Ho Ryou),이무형(Moo Hyoung Lee) 경희대학교 경희의료원 1996 慶熙醫學 Vol.12 No.2

알부틴과 베타-글루코시다제를 함유하는 미백 화장품의 항멜라닌 효과

유박린 ( Bark-lin Lew ),류지호 ( Ji-ho Ryou ),이무형 ( Mu-hyoung Lee ) 대한화장품학회 2004 대한화장품학회지 Vol.30 No.3

알부틴은 glycosylated hydroquinone으로 여러 식물들에서, 고농도로 발견되며 비교적 안정한 물질이다. 이는 멜라닌화를 억제하는 작용이 있는 것으로 보고되어 있고, 베타-글루코시다제에 의해 포도당과 hydroquinone으로 쉽게 가수분해된다. Hydroquinone 역시 우수한 항멜라닌화 작용을 가지나, 여러 가지 부작용들로 인해 그 사용이 제한적이거나 주의를 요한다. 이 연구는 알부틴과 베타-글루코시다제를 포함하는 미백 화장품이 자외선 조사로 유도된 색소 침착을 억제하는 효과를 알아보고자 하였다. 대상은 10명의 건강한 성인으로 등 부위에 자외선 B를 조사하여 색소 침착 병변을 만들었고 이 병변들은 세 그룹: 알부틴과 베타-글루코시다제를 함유하는 미백 화장품 도포군, vehicle 대조군, no-application 대조군으로 나누었다. 알부틴과 베타-글주코시다제를 함유하는 미백 화장품 도포군은 no-application 대조군에 비교하여 색소 침착을 50.17% 억제하였다(N = 10: P < 0.05). 따라서 알부틴과 베타-글루코시다제를 함유하는 미백 화장품이 기미 등 자외선 조사로 유도되거나 악화되는 과색소성 질환에 효과적으로 사용될 수 있겠다. Arbutin is a glycosylated hydroquinone found at high concentration in certain plants capable of surviving extreme and sustained dehydration. It has been reported to have an inhibitory effect of melanogenesis and to be hydrolyzed easily to yield glucose and hydroquinone by β-glucosidase. While hydroquinone also has an anti-melanogenic effect, however, is carefully used as a topical whitening agent because of side effects. The present study was undertaken to examine the inhibitory effect of an whitening agent containing arbutin and β-glucosidase on UV radiation induced pigmentation in human skin. Experimental subjects were UVB-irradiated on the back. UVB-irradiated areas were assigned to three groups: arbutin and β- glucosidase treated group, vehicle control, and no-application control. Arbutin and β-glucosidase treatment inhibited pigmentation by 50.17 percent, compared with the controls (N : 10; P < 0.05). These results suggest that the whitening agent containing arbutin and β-glucosidase may be used as an agent to inhibit melanin formation induced by UV radiation.

Intense Pulsed Light Quantum SR(TM)을 이용한 안면부 피부노화에 대한 치료 3예

서동혜 ( Dong Hye Suh ),장가연 ( Ka Yeun Chang ),류지호 ( Ji Ho Ryou ),이상준 ( Sang Jun Lee ),송계용 ( Kye Yong Song ) 대한피부과학회 2004 대한피부과학회지 Vol.42 No.8

Phorbol 12-myristate 13-acetate(PMA)가 첨가된 배양액과 생리적 분열 촉진제가 첨가된 배양액에서 배양된 인체 멜라닌세포의 생물학적 특성 비교

이무형,류지호,전범진 대한피부과학회 1999 대한피부과학회지 Vol.37 No.2

Background : The growth of cells is closely related to components in a culture medium. There are many reports about cellular characteristics of melanocytes grown in a PMA-contained medium. However, only a few reports have been studied by using a physiologic mitagens-contained medium. To understand melanocyte in vivo, it is necessary to know the cellular biology of melanocytes grown in a physiologic mitogens-contained medium. Objective : To investigate any differences between melanocytes grown in phorbol 12-myristate 13-acetate(PMA)-contained medium and in physiologic mitogens-contained medium. Method : We examined morlahology, number and melanin contents of cultured human melanocytes grown in a PMA-contained medium and physiologic mitogens-, such as bFGF, ET-1 and a α-MSH contained medium. Result : The results are summarized as follows I. There were no sigrtificant morphologic differences between cells in PMA-contained medium and in physiologic mitogens-contained medium. 2. The number of melanacytes were significantly more numerous in PMA-contained medium on the 2nd day (p $lt;0.05), but significantly less numerous in the same medium on the 6th day (p $lt;0.05). So, the proliferation rate of melanocytes in PMA-contained medium became lower than in physiologic mitogens-contained medium as time went by. 3. Melanacytes grown in PMA-contained medium had significantly increased melanin contents regardless of the time (p $lt;0.05). Conclusion : The proliferation of melanocytes was better in physiologic mitogens-contained medium, the melanization was higher in melanocytes of PMA-contained medium.

배양 인체 각질형성세포에서 Nerve Growth Factor ( NGF ) 수용체 발현과 NGF 가 세포 증식에 미치는 영향에 관한 연구

박재경,김낙인,류지호,임덕우 대한피부과학회 2001 대한피부과학회지 Vol.39 No.2

Background: Nerve growth factor (NGF) is a neurotrophic polypeptide necessary for the survival and growth of some central neurons, as well as sensory afferent and sympathetic neurons. In addition to its actions on the nervous system, it also has a significant biologic effects on cells of the immune-inflammatory compartment. Recent studies suggest that NGF is an important autocrine growth factor and survival factor for keratinocytes which express both high- and low-affinity receptors for NGF. Objective: The purpose of this study is to detect NFG receptors an cultured human keratinocytes and to evaluate the effect of NGF on proliferation of cultured human keratinocytes. Methods: Cultured human keratinocytes were examined for the expression of high affinity receptor TrkA and low affinity receptor p75 by Northern blot, Western blot and immunocytochemistry, The effects of NGF on proliferation of cultured human keratinocytes were also evaluated. To specify the NGF effect on proliferation of human keratinocytes, excess of anti-NGF neutralizing polyclonal antibody was added. Results: 1) NGF significantly stimulated the proliferation of keratinocytes in both 1% of keratinocyte growth supplement (KGS)-added medium (100ng/ml) and 0.2% KGS-added media(50, 100, 500ng/ml), (p$lt;0.05). The cell number was dose-dependently increased in 0.2% KGS-added media. 2) Whenever we added 500 ng/ml of anti-NGF polyclonal antibody to the growth media, the cell number was statistically higher in 100ng/ml NGF-added group of 1% KGS-added medium, but there was not any statistical significance in 0.2% KGS-added media group. 3) Immunocytochemical staining with specific antibodies to TrkA and p75 revealed positive findings for these receptors, but TrkB and TrkC were not detected. 4) We could not detect both the mRNA and protein of TrkA and p75 by Northern and Western blot methods. Conclusion: These results suggest that both high affinity- and low affinity receptors for NGF are expressed in cultured human keratinocytes and NGF can induce keratinocyte proliferation.