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대류·복사 연성시뮬레이션을 통한 옥외 온열환경 평가 기법
류민경(Minkyung RYU),임종연(Jong-Yeon Lim),황효근(Hyo-Keun Hwang),송두삼(Doo-Sam Song) 대한설비공학회 2009 대한설비공학회 학술발표대회논문집 Vol.2009 No.-
Deterioration of the outdoor thermal environment in urban areas such as heat island has become worse due to urbanization and overpopulation, etc. In this study, a new method which is coupled simulation of convection and radiation to evaluate outdoor thermal environment in urban area will be proposed. Because the solar radiation affects on outdoor thermal environment massively, therefore the radiation calculation is very important in outdoor thermal environment prediction. The coupled simulation proposed in this study can assess the outdoor thermal environment with accurate.
조피볼락(Sebastes schlegeli) 선충(Nematode: Philometridae)에 대한분자생물학적 동정 및 PCR 검출법 개발
서한길 ( Hangill Seo ),서정수 ( Jungsoo Seo ),류민경 ( Minkyung Ryu ),이은혜 ( Eunhye Lee ),정승희 ( Sunghee Jung ),한현자 ( Hyunja Han ) 한국수산과학회(구 한국수산학회) 2015 한국수산과학회지 Vol.48 No.5
Nematode infection in the epithelial tissue of cultured rockfish Sebastes schlegeli was first reported in 2012. Since then, nematode infections have caused serious economic losses in rockfish aquaculture on the west coast of Korea. Taxonomic and life cycle information for this parasite are currently unknown. In this study, 18S rRNA and cytochrome c oxidase subunit I (COI) genes were used for molecular identification and polymerase chain reaction (PCR) to detect the invisible stages of this parasite. Nucleotide sequences of the 18S rRNA of the rockfish nematode showed 98% identity with that of Philometra morii. Therefore, this rockfish nematode was classified to the Philometridae family. However, we could not identify it to genus level using 18S rRNA. Its COI nucleotide sequences shared 85% and 82% identities with those of Bursaphelenchus sinensis and Philometra overstreeti, respectively. In addition, two gene-specific primer sets were designed based on the 18S rRNA gene to detect the intermediate host and nematode larvae. These primers were specific to this rockfish nematode without cross-reacting to other pathogens. The detection limit of the PCR assay using these primers was 1,000 copies of nematoda plasmid DNA. Therefore, the PCR assay described here is suitable for the detection of nematode DNA within rockfish. In addition, this PCR assay could be used to detect nematode larvae and the intermediate host.
최광환(Kwang-Hwan Choi),김민수(Minsu Kim),윤지원(Ji Won Yoon),정진솔(Jinsol Jeong),류민경(Minkyung Ryu),조철훈(Cheorun Jo),이창규(Chang-Kyu Lee) 한국축산식품학회 2020 한국축산식품학회지 Vol.40 No.5
The muscle stem cells of domestic animals are of interest to researchers in the food and biotechnology industries for the production of cultured meat. For producing cultured meat, it is crucial for muscle stem cells to be efficiently isolated and stably maintained in vitro on a large scale. In the present study, we aimed to optimize the method for the enrichment of pig muscle stem cells using a magnetic-activated cell sorting (MACS) system. Pig muscle stem cells were collected from the biceps femoris muscles of 14 d-old pigs of three breeds [Landrace×Yorkshire×Duroc (LYD), Berkshire, and Korean native pigs] and cultured in skeletal muscle growth medium-2 (SkGM-2) supplemented with epidermal growth factor (EGF), dexamethasone, and a p38 inhibitor (SB203580). Approximately 30% of total cultured cells were nonmyogenic cells in the absence of purification in our system, as determined by immunostaining for cluster of differentiation 56 (CD56) and CD29, which are known markers of muscle stem cells. Interestingly, following MACS isolation using the CD29 antibody, the proportion of CD56+/CD29+ muscle stem cells was significantly increased (91.5±2.40%), and the proportion of CD56 single-positive nonmyogenic cells was dramatically decreased. Furthermore, we verified that this method worked well for purifying muscle stem cells in the three pig breeds. Accordingly, we found that CD29 is a valuable candidate among the various marker genes for the isolation of pig muscle stem cells and developed a simple sorting method based on a single antibody to this protein.