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무증자 발아흑미를 이용한 막걸리 제조 시 온도가 미치는 영향
김다래(Da-Rae Kim),서보미(Bo-Mi Seo),노민희(Min-Hee Noh),김영완(Young-Wan Kim) 한국생물공학회 2012 KSBB Journal Vol.27 No.4
In this study we investigated the effect of temperature on the two-stage fermentation of Makgeolli using uncooked germinated black rice. The fermentation processes were conducted at 15℃ for three weeks and 25℃ for 7 days. The pH of Makgeolli at 25℃ increased from pH3.0 to pH 4.2, which was consistent with that at 15℃. In contrast total acidity of Makgeolli at 15℃ was about half of that at 25℃ (0.36% and 0.59%, respectively). By the 7 days-fermentation at 25℃, 11% of alcohol was produced, whereas three weeks were required for the same alcohol production at 15℃. In the case of sugar contents, the amounts of total glucose-equivalent reducing sugars and glucose increased at the end of the fermentation at 25℃ up to 2.25 mg/mL and 3.4 mM, respectively, whereas those at 15℃ were maintained at very low levels (0.18 mg/mL and 0.1 mM, respectively). Such limited supplement of sugars at 15℃ seemed to affect metabolism of yeast, resulting in different composition of organic acid. At 25℃, citric acid that was 73.4 ppm at the initial fermentation was consumed completely, whereas 20 ppm of citiric acid was remained at 15℃. In addition, acetic acid and lactic acid in Makgealli at 15℃ were 53% and 14% of those at 25℃.
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대두와 Magnesium 급여가 당뇨쥐의 혈청과 간의 단백질 농도 및 효소활성에 미치는 영향
고진복,김재영,노민희 신라대학교 자연과학연구소 1996 自然科學論文集 Vol.2 No.-
Streptozotocin으로 유발시킨 당뇨쥐에 20% 생콩 및 20% 생콩에 마그네슘을 첨가한 식이로 4주간 사육하였다. 체중, 간, 신장 및 비장의 무게, 간과 혈청중 단백질농도, 그리고 혈청중 GOT, GPT 및 alkaline phosphatase의 활성에 미치는 영향을 조사하였다. 당뇨를 유발한 실험군은 당뇨병으로 인한 대사장애로 대조군에 비해 체중이 감소되었다. 간, 신장 및 비장의 무게는 대조군과 모든 당뇨군이 비슷하였으나, 체중에 대한 간, 비장 및 신장무게의 % 비율은 모든 당뇨군이 대조군보다 유의(p<0.05)하게 증가하였다. 간의 총 단백질과 albumin 농도는 대조군에 비해 모든 실험군에서 유의(p<0.05)하게 감소하였으나, 혈청의 총 단백질과 albumin의 농도 및 A/G ratio는 대조군과 각 당뇨실험군이 비슷한 수준을 보였다. Hemoglobin 농도와 hematocrit 치는 정상수준이었다. 혈청내 GPT 및 alkaline prosphatase의 활성은 대조군에 비해 모든 당뇨유발군이 유의(p<0.05)하게 증가되었다. 그리고 alkaline phosphatase의 활성은 당뇨대조군에 비해 당뇨생콩군과 당뇨생콩+Mg군은 다소 감소되었다. The effects of raw soy flour(RSF) and RSF with magnesium supplementation(RSF+ Mg) feeding on protein concentrations of liver and serum, and GOT, GPT and alkaline phosphatase activities of serum in diabetic versus nondiabetic rats were studied. Male rats(Spraque-Dawley ; mean weight 332.8±19.3g) were assigned to four dietary groups and fed experimental dietary for 28 days. For each experiment, some rats were injected with streptozotocin in intraperitonium(i.p.) to induce diabets, and the other rats were injected with buffer in i.p. as a nondiabetic group. The body weights of diabetic control, RSF and RSF+Mg groups were significantly decreased(p<0.05), but the total weights of liver, kidney and spleen in all the groups did not change compared with those of the nondiabetic group. Total protein and albumin concentrations of liver in all the groups were significantly lower(p<0.05) compared with those of the nondiabetic group. GPT and alkaline phosphatase activities in serum of all the groups were much more increased(p<0.05) than those of the nondiabetic group, but those of RSF and RSF+ Mg groups were similar to those of the diabetic control group.
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