http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
자궁경부암을 유발하는 HPV E6 단백질에 의한 p73 단백질의 불활성화 : p53과 무관한 E6의 새로운 기능
남궁성은(Sung Eun Namkoong),김승조(Seung Jo Kim),김은주(Eun Joo Kim),엄수종(Soo Jong Um),박종섭(Jong Sup Park) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.11
Objective: Human papillomavirus (HPV) is strongly implicated as a causative agent in the etiology of cervical cancer. Of its gene products, E6 and E7 oncoproteins play major roles by inactivation of cellular p53 and pRb tumor suppressor proteins, respectively. However, it has been recently suggested that p53 and/or pRb-independent functions of E6 and E7 are involved in cervical carcinogenesis. The purpose of this study is to identify novel a cellular target, p73, of E6 and to determine how E6 inactivates p73 function, Methods: The interaction between E6 and p73 were identified by the yeast two-hybrid assay in vivo and the GST pull-down assay in vitro. The function of the interaction was determined by transient transfections using p21 promoter-CAT reporter plasmid. The molecular mechanism underlying the functional significance of the interaction was further assessed by in vivo and in vitro protein degradation assays, and gel mobility shift assays. Results: Yeast two-hybrid and GST pull-down assays indicate a physical interaction between p73 and either HPV-16 or HPV-11 E6 proteins in vivo and in vitro, respectively. Transactivation domain (amino acid residues 1-49) is found to be absolutely required for this interaction. Transient co-expression of E6 significantly inhibits the p73-mediated activation of p21 promoter in a p53-defective C33A cell line. Using Ga14-p73 fusion protein, we demonstrate that E6 inhibition of p73 transactivation function is independent of sequence-specific DNA binding, which is confirmed by direct electrophoretic mobility shift assay. Moreover, E6 inhibits p73 function by interfering with the activity of the amino-terminal activation domain. The protein degradation assays in vivo and in vitro indicate that p73, unlike p53, is not susceptible to E6-dependent proteolysis. Conclusion: Throughout this study, we identified p73 as a novel cellular target of HPV-E6 protein and found that E6 binds p73 through the amino-terminal transactivation domain, and inhibits its transactivation function independent of the protein degradation and DNA binding. These overall results, consequently, suggest that in addition to the inactivation of p53, the functional interference of p73 by HPV-E6 may, at least in part, contribute to E6-mediated cellular transformation.
[재료] 자동차용 합금화 용융아연도금강판의 도금층특성에 미치는 합금화욜처리 및 도금욕 조성의 영향
남궁성(Sung Namkoong),문만빈(Manbeen Moon) 한국자동차공학회 1999 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1999 No.11_2
As GA(Hot dip galvanncaled sleel sheet has good corrosion resistance weldability and paintability as well as excellent formability it's demand is rapidly increasing for automotive panel However. since GA coated layer is composed of several kinds of brittle Fe-Zn Metallic compounds which are susceptible to powdoring in case of press forming. very careful control of manufacturing conditions such as galvanncaling heat-treatment or bath composition is essential to meet with the required quality of automobile use<br/> In the study the requirced charateristics of automotive panel is practically surveyed in detail and the appropriate manufacturing conditions of galvannealing or bath composition with the newly developed induction heating type furnace are experimentally investigated by using various analysing and simulating equipments including hot dipping and galvannealing simulation system.<br/>
고위험 자궁경부암 제 I B , II A 및 II B기 환자에서 수술전 선행 항암요법의 효과
남궁성은(SE Namkoong),배석년(SN Bae),안웅식(WS An),박종섭(JS Park),김진우(JW Kim),한구택(GT Han),이준모(JM Lee),김승조(SJ Kim) 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.12
To evaluate the potential role of preoperative neoadjuvant Chemotherapy in Patients with cervical cancer, 92 patients with locally advanced cervical cancer stage IB, IIa and IIB who had completed 4years of follow-up(from January 1985 to December 1989) after treated with chemotherapy followed by radical hysterectomy and pelvic lymphnode dissection at the Division of Gynecologi Oncology, Catholic University Medical College Seuol, Korea were selected in this study. The treatmentregimen was consisted of cisplatin 80mg/m2, bleomycin 15mg/m2 and viblastin 4mg/m2 given as a course (3 weeks interval) of neoadjuvant chemotherapy. All patient were evaluated by clinical and histological response to chemotherapy. The overall clinical response rate was 82.6% (28.3% of complete response and 54.3% of partial response). The chemotherapeutic response was more favorable in squamous cell carcinomas (86.9%) than in adenocarcinomas(37.5% P=0.010) The response rate confirmed by surgical specimen was 15% including microscopic and no residual disease. Pelvic lymphnode metastasis were found in 17.4%(16/92) of the patients and all nodal metastasis were found among the patients who had a partial response or a stable disease, and olny one patient was found in those with a complete response (p=0.0001). All patients were passed 4 years follow-up and the 4 year tumor free survival rate in patients with complete response. partial response and stable disease were 96.2%(25/26), 88%(44/50), and 37.5%(6/16). The 4 year tumor free survival rate of high risk cervical cancer stage IB, IIA, and IIB were 87.9% (36/11), 83.4%(30/36) and 60%(9/15). This study suggest that preoperative adjuvant chemotherapy may be beneficial in reducing pelvic lymphnode metastasis, reducing recurrences and prolonging the survival of the patients with locally advanced high risk cervical cancer stage I and II.
SiHa 세포주에서 비소화합물 ( As2O3 , As4O6 ) 투여 후 cDNA microarray 를 이용한 유전자 발현의 변화
남궁성은(Sung Eun Namkoong),서미영(Mi Young Seo),박은경(Eun Kyung Park),팽기영(Gi Young Pang),김종국(Chong Kook Kim),박용석(Yong Serk Park),안웅식(Woong Shick Ahn),이준모(Jun Mo Lee),김도강(Do Gang Kim) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.7
Objective : To obtain information on the growth inhibition effect of arsenic compounds and gene expression profiles using cDNA microarray technique in SiHa cell lines. Methods : We cultured 103 SiHa cell in 96 well plate and we investigated growth inhibition effects using MTT assay and also we performed gene expression profile experiment using 384 cDNA chip in SiHa cell after exposure of arsenics (As2O3, As4 O6 - 1 μM) for 48 hrs. Results : Arsenics (As2O3, As4 O6) inhibit the growth of SiHa cells (As2O3: 0.5, 1, 2, 3, 4, 5 μM - 9.2, 56, 89, 93, 96, 96%, As4O6: 0.5, 1, 2, 3, 4, 5 μM- 54, 84, 84, 85, 85, 87%) in 4 days culture. As2O3 and As4O6 induced apoptosis in SiHa cells. After exposure of As2O3, 47 genes were changed more than 2 times (eg, thymidylate synthetase, cyclin B1, CDC 20). In case of As4O6, 78 genes were changed more than 2 times (eg, CDC 20, cyclin B1, primase, proliferating cell nuclear antigen). Conclusion : we observed arsenic compound (As2O3, As4 O6) inhibit the growth of SiHa cell. In gene expression profiling experiment, 78 genes was changed the expression level 2 times more than that of reference RNA after treatment of As4O6 and 47 genes after treatment of As2O3. Through these result, we thought more study need in functional genomics after arsenic treated cervical cancer cells.