Porcine Ryanodine Receptor 유전자의 Mutation 을 확인하기 위한 Double Tube Allele Specific Pimer PCR 의 이용

김철욱,조광근,최윤재,박외선,권은정 ( C . W . Kim,K . K . Cho,Y . J . Choi,W . S . Park,E . J . Kwon ) 한국축산학회 1998 한국축산학회지 Vol.40 No.3

A double tube allele specific PCR assay was developed for a rapid and accurate detection of the porcine Ryanodine receptor genotypes using the allele specific pimer PCR. The assay, a modification of Zinovieva` single tube allele specific(STAS) PCR, reduced significantly primer dieter artificial band and determined allelic variations in simple, accurate and cost-effective ways. PCR primers were designed as followed ; two allele-specific `internal` primers[Int 1 for the normal allele N 5`-AGTAATGAGATCTTGGTTGGA(G→T)CG-3`, Int 2 for the mutant allele n : 5`-GTGCAATGGTGTGGCCG (T→C)GT-3`] were recognized with their 3`nucleotide the allelic variation. In order to increase the allele specific annealing an additional mismatch at position 3 from the 3` end of each primer was introduced. The common `external` primers(Ext 1, Ext 2) were located at different distances from the point mutation(nt 1843, C→T), so that different fragment lengths were generated for each allele. In the case of the gene, in normal(NN) and mutant (nn) homozygotes, only one fragment of 533 by or 823 bp, respectively, was amplified, whereas in heterozygotes (Nn) both fragments were detected. Independently from the genotype, an additional product(1312 bp) of the common `external` primers was amplified in. the single tube method. The use of the allele specific PCR for detecting allelic nucleotide exchanges offers some obvious advantages the presence of restriction endonuclease sites is not required and the allelic variations are visualized by simple agarose gel electrophoresis. As compared with the PCR-RFLP this modified method is simple, reliable and time and cost effective and thus can be applied in large scale to establish the program for testing and removal of the genes associated with the breeding of swine.

돼지의 번식형질에 대한 유전 및 환경적 효과 상호작용에 관한 연구

김철욱,강대진,박충생 ( C . H . Kim,D . J . Kang,C . S . Park ) 한국축산학회 1983 한국축산학회지 Vol.25 No.1

This study was conducted to determine the main effects and interactions of breed, furrowing season and parity on swine reproductive traits: litter size at birth and weaning, pig and litter weight at birth, 21 days and weaning on the basis of the data obtained from 1047 litters of Berkshire and Landrace born from 1976 to 1980 at Sacheon Branch, National Livestock Station. The data were analysized by the least square method and results obtained are summarized as follows: 1. Landrace was heavier by 10% in litter weight at weaning, 17% in litter weight at 21 days, 10% in litter weight at birth, 23% in pig weight at 21 days than Berkshire. 2. The effect of breed for each litter weight and pig weight at 21 days was highly significant at 1% level. 3. The effect of furrowing year was highly significant for all the traits studied without any consistent pattern as the year passed. 4. The reproductive performance of the pigs born in the spring was significantly higher (p$lt;.001) than that in the fall except for the pig weight and litter size at weaning. 5. The litter size at birth or weaning was significantly larger (.1%) in the groups of the 2nd to 6th parity than in the other groups. All the body weight traits studied were significantly (.1%) superior in the 2nd 4th, the 5th - 6th, the 7th and over, and the 1st parity in that order. 6. The pig weight at weaning in Berkshire was significantly heavier in the spring-born group. The litter size at weaning and litter weight at birth in Landrace was significantly higher in the spring-born groups than in the fall-born ones. 7. The effect of breed x parity interaction was nonsignificant for the traits studied.

닥나무의 사료적 가치에 관한 연구

정형승,한인규,유동준,김철욱 ( H . S . Chung,I . K . Han,D . J . Yoo,C . W . Kim ) 한국축산학회 1985 한국축산학회지 Vol.27 No.7

In order to study the feeding value of Broussonetia kazinoki, Siebold, the investigation on its characteristics and the feeding trial were carried out from April, 1974 to October, 1984. The growth of the tree was accelerated from age of 3 year. Green leaf and rhytidone was lower in C. protein content, but higher in c. fiber content than fallen leaf and leaf before fallen. Most of amino acids were higher in leaf fallen, except for cystine and aspartic acid, than in fallen leaf. The results obtained from the feeding trial indicated that the supplementation of Broussonetia kazinoki, Siebold to the growing-finishing pig diet resulted in improved weight gain (p$lt;0.05), feed conversion (p$lt;0.05) and feed cost per ㎏ gain.

유전 및 환경적 요인에 따른 세포유전학적 연구 1 . 계통별 염색체 이상

김철욱(C . W . Kim),손진기(J . K . Son),(N . S . Fachheimer) 한국축산학회 1991 한국축산학회지 Vol.33 No.2

This study has examined the fertility rate, the viability and the incidence of heteroploidy of each group of Japanese quails divided by their line using 1,349 early embryos of the Japanese quails selected and bred for the levels of total plasma phosphorus. The fertility rate was geneally high in high plasma phosphorus level line and randombred line. but it was very low in low plasma phosphorus level line. The frequency of heteroploidy averaged about 12.08% and LP yielded much more heteroploid embryos than HP or RP

염색체분염법 ( G- banding ) 에 의한 돼지의 품종별 유전적 표지인자 구명에 관한 연구

김철욱(C . W . Kim),손시환(S . H . Sohn) 한국축산학회 1995 한국축산학회지 Vol.37 No.1

To identity genetic martcers of several swine breeds, the G-banding patterns were analyzed. The peripheral blood cells which were collected from the jugular vein were cultured and G-banding patterns were analyzed from 50 Yorkshire, 50 Landrace, and 50 Duroc individuals. Swine chromosomes consist of metacentric, submetacentric, and acrocentric chromosomes. The number of swine chromosomes are 38(2n). According to the G-banding analysis, most of chromosomes had a distinct and regular band pattern. The total number of G-bands in swine was about 250 bands, of which 110 bands were dark hands. Several specific G-band patterns were found among breeds. In Landrace, chromosome I had a distinctive proximal dark band on the p-ann and chromosome 13 to chromosome 18 also had manifest dark bands at the centromere position. On the other hand, Yorkshire and Duroc had irregular banding patterns at these sites. In addition, the G-bands of the nucleolar organizer region (NOR) which was located at centromere of chromosome 10 showed different patterns among breeds. Landrace and Duroc had a broad Gght band in this position. However, Yorkshire had a narrow light band. The specific banding pattern was consistent and characteristic among all presented breeds. Therefore we present these specific G-band patterns as chromosomal markers for several swine breeds.

유전 및 환경적 요인에 따른 세포유전학적 연구 2 . 난중 및 Sex 별 염색체 이상

김철욱(C . W . Kim),손진기(J . K . Son),(N . S . Fachheimer) 한국축산학회 1991 한국축산학회지 Vol.33 No.2

This study has examined the fertility rate, the viability and the incidence of heteroploidy of each group of Japanese quails divided by their line using 1,349 early embryos of the Japanese quails were selected and bred for the levels of total plasma phosphorus. As a results, there was no difference in frequencies of heteroploidy of each egg weight and sex.

유전 및 환경적 요인에 따른 세포유전학적 연구 4 . 산란능력에 따른 염색체 이상

김철욱(C . W . Kim),손진기(J . K . Son),(N . S . Fechheimer) 한국축산학회 1991 한국축산학회지 Vol.33 No.3

This study has examined the fertility rate, the viability and the incidence of heteroploidy of each group of Japanese quails divided by their egg productivity using 1,349 early embryos of the Japanese quails which were selected and bred for the levels of total plasma phosphorus. There were differences in the frequencies of heteroploidy of each egg productivity level, EP3 whose egg productivity was superior yielded more heteroploid embryos than EP1 or EP2.

다배란과 수정란이식에 의한 젖소 산유능력의 유전적 변화에 관한 연구 1 . 선발방법에 따른 선발효과

김철욱(C . W . Kim),강대진(D . J . Kang),박충생(C . S . Park),이정규(J . K . Lee) 한국축산학회 1988 한국축산학회지 Vol.30 No.1

Thanks to the recent technical development of embryo transfer, it has become possible to produce many good offsprings from the genetically highly productive dairy cattle in a short period, With the help of this technical development this study aims to analysis genetic change by use of multiple evulation and embryo transfer (MOST), Under the condition that the heritability of milk yield is 0.25, the number of donors per male is 20, and the number of embryo transfers per donor is 20, comparing their respective selection effects of dam I, dam II, dam I + dam II, and the females and males selected according to the genetic selection index, 1 have found that the annual genetic change of the group selected by the genetic selection index is the highest. And the genetic change is the highest when one male per half-sib is selected.

교배조합과 산차 및 이유일령이 돼지의 생산형질에 미치는 영향

김철욱(C . W . Kim),진상근(S . K . Jin),김두환(D . H . Kim) 한국축산학회 1995 한국축산학회지 Vol.37 No.5

This study was carried out to estimate the effects of mating type, parity and weaning age on productive traits in swine. The body weights by increasing age, average daily gains, days to 110 ㎏ body weight and feed Conversion for 155 piglets produced in the first and over second parity from mating systems breeding Landrace×Yorkshire sows to Duroc boats, Landrace×Yorkshire sows to Landrace boar, Landrace×Yorkshire sows to Yorkshire boars and Landrace×Yorkshire sows to Duroc×Hampshire boars were investigated from their weaning age at 32 days or 28 days to the time they reached 110 ㎏ holly weight. The body weight at weaning was the highest at 9.2 ㎏ in the LY×Y mating type, but was the lightest at 5.5 ㎏ in LY×D mating type. Body weight gain from weaning to 70 days of age was the highest at 21.0 ㎏ in LY×D mating type, but was the lowest at 18.7 ㎏ in LY×Y mating type among the mating types. Body weight at 150 days of age was better in the group weaned at 32 days than at 28 days. The interactions among mating type and parity, body weight at different ages and weight gain were not statistically significant, but performance was better in the fitst and over second parity of LY×D mating type than in the other groups. Weight gain from weaning to 70 days of age was significantly better in groups weaned at 32 days than at 28 days of age. There was not a significant difference in the days to 110 ㎏ body weight according to the mating type, parity and weaning age, but was he shortest at 172 days in LY×Y mating type among the mating types. Feed conversion was not significant different according to the mating type and weaning age. but was significantly better in the over second parity than in the first parity.

교배조합과 산차 및 이유일령이 돼지의 도체형질에 미치는 영향

김철욱(C . W . Kim),진상근(S . K . Jin),김두환(D . H . Kim) 한국축산학회 1995 한국축산학회지 Vol.37 No.5

This study was carried out to investigate the effect of mating type, parity and weaning age on carcass traits as a dressing percent, backfat thickness of carcass and percent of carcass grade in swine. A total of I55 carcass were tested to compare the carcass traits with mating type breeding Landrace×Yorkshire sows to Duroc boar, Landrace×Yorkshire sows to Landrace boar, Landrace×Yorkshire sows to Yorkshire boar and Landrace×Yorkshire sows to Duroc×Hampshire boar, and first parity and over second parity, weaning at 28days and 32days. Dressing percentage was not significantly different among mating type, parity and weaning age and showed to be about 62%. Backfat of carcass was significantly thinner as 9.1 ㎜ in LY×Y mating type than in the other mating types, and the most thickness as 15.8㎜ was found in LY×DH mating type, but was not significantly different in parity and weaning age. There were not significant differences for the percent of carcass grade among mating type, parity and weaning age. Total percent of A and B grade showed to be in the order of 59.9% in LY×D, 48.7% 1_Y×L, 45.6% LY×Y and 32.5% LY×DH. In the interaction effects among mating type parity and weaning age, there were not significant differences for percent of dressing and carcass grade, but was significantly difference for backfat thickness of carcass in the first and over second parity of LY×L mating type. The percent of age carcass grade D was significantly higher as 21.5%r in the over second parity than in the first parity of LY×D mating type.