디지털 타이머를 이용한 인터넷 기반의 원격제어 및 모니터링 (pp.245-248)

김은주(EJ Kim),강신영(SY Kang),박철수(CS Park),임영철(YC Lim),김광헌(GW Kim) 대한전기학회 2001 대한전기학회 학술대회 논문집 Vol.2001 No.10

한국인 혈우병 A 가계에 있어 St14 VNTR 부위 DNA Polymorphism 양상 및 이를 이용한 혈우병 A 보인자진단 및 산전진단

김석현(SH Kim),김정구(JK Kim),문신용(SY Moon),이진용(JY Lee),김은주(EJ Kim) 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.3

At present, because of enormous variety of mutations in hemophilia A, carrier detection and prenatal diagnosis by DNA analysis has been relied almost always on indirect detection using linkage analysis of DNA polymorphisms withim or near to the factor VIII gene. However, there is marked ethnic variation in the incidence of heterozygosity for a given DNA polymorphism. So it is very important to find out which DNA polymorphism pattern is useful in Korean families with hemophilia A for carrier detection and prenatal diagnosis. To identify the usefulness of DNA polymorphism in St14 VNTR locus for carrier detection and prenatal diagnosis of hemophilia A in Korean populations, we have analysed the DNA polymorphism in St14 VNTR locus in 80 Korean families with hemophilia A using polymerase chain reaction. We could identify 14 alleles in subjects studied, which ranges from 620 bp to 2830 bp. Expected heterozygosity rate, calculated from the allele frequencies, was 78.7%, and observed heterozygosity rate was 71.3% (57/80). Carrier detection was performed in 43 women from families informative with St14 VNTR : Seventeen women were diagnosed as non-carriers, 11 women as carriers. And 15 women were suspected to be carriers since they were from families of sporadic cases of hemophilia A. And prenatal diagnosis was done in 4 pregnant carrier women : noe fetus proved to be normal males, two fetuses to be normal females, and one to be a carrier. And five pregnant women, suspected to be carrier since they were from families of sporadic cases of hemophilia A, underwent prenatal diagnosis : One fetus was diagnosed as a normal mali, one as a normal female, two as possible carriers, and one as a possible affeted mali, whom the analysis of factor VIII level in fetal blood by cordocentesis revealed to be affected by hemophilia A. These data indicate that PCR-based analysis of St14 VNTR is very useful for the carrier detection and prenatal diagnosis of hemophilia A in Korea.

자궁경부암 환자에서 혈청내 진단 물질로서 in vitro translated HPV-16 E7 단백질과 GST-fusion HPV-16 E7 단백질의 임상적 효용성 비교

박종섭(JS Park),김태연(TY Kim),김은주(EJ Kim),김찬주(CJ Kim),박동춘(DC Park),박순희(SH Park),김승조(SJ Kim),남궁성은(SE Namkoong),엄수종(SJ Um) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.7

Objective: Recently, a variety of HPV-related proteins have been synthesized and their utility as diagnostic and prognostic markers in cervical cancers is needed. The ability to generate preparative amounts of HPV-16 E7 proteins may have implications for the development of a serologic assay to detect anti-HPV-16 virion immune responses. The purpose of the study is to improve the way of proper management of the cervical cancer by investigating the clinical utility of in vitro translated HPV-16 E7 protein and GST-fusion HPV-16 E7 protein as the serologic marker through comparing the antibody reactions in the sera of the patients. Methods: The serologic responses were investigated in Korean women with cervical neoplasia by radioimmunoprecipitation assay (RIPA) using in vitro translated HPV-16 E7 protein and ELISA using GST-fusion HPV-16 E7 protein. PCR using E6 type-specific primers for HPV-16/18 were used to determine the presence and type of HPV infection (normal controls; 15 cases, preinvasive lesions; 15 cases, and invasive cervical cancers; HPV-16 DNA positive 78 cases and HPV-16 DNA negative 22 cases). Results: The sera of 39% (39/100) of patients with cervical cancer were RIPA positive for in vitro translated HPV-16 E7 protein [HPV-16 DNA positive cases; 41% (32/78), HPV-16 DNA negative cases; 32% (7/22)]. The sera of 29% (29/100) of cervical cancers were ELISA positive for HPV-16 GST-fusion E7 protein [HPV-16 DNA positive cases; 33% (26/78), HPV-16 DNA negative cases; 14% (3/22)]. By the two different methods, 12 cases were positively matched and 32 cases were negatively matched. The positivities for in vitrotranslated HPV-16 E7 protein in the patients with cervical cancer were 14% (7/49) in stage I, 59% (26/44) in stage II, and 86% (6/7) in stage III/IV, by significantly correlating with the advancing stage of the disease (P<0.01). Conclusions: These data suggests that the considerable number of patients with cervical neoplasia generated positive antibody response to in vitro translated protein and GST-fusion protein of HPV-16 E7. In contrast to RIPA method using in vitro translated protein, ELISA method using GST-fusion protein has several advantages for clinical application; use of non-isotope, easy preparation to produce a sufficient of protein, available large scale study. But the positive rates to in vitro translated HPV-16 E7 protein in the patients of cervical cancer were higher in general than those to GST-fusion HPV-16 proteins. Furthermore, the antibody positivities to in vitro translated HPV-16 E7 protein were increased by advancing clinical stage of disease. These HPV-16 E7 proteins might be the disease-specific markers which could be useful in adjunctive diagnostic assay and seroepidemiologic study of HPV-related cervical neoplasia.

산전 초음파로 진단된 태반혈관종 1 례

김상국(SK Kim),정주섭(JS Chung),배임철(IC Bae),김은주(EJ Kim),배국환(KH Bae) 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.9

Benign chorioangioma of the placenta is the most common primary tumor of the placenta followed by the hydatidiform mole and choriocarcinoma. Only two nontrophoblastic primary placental tumors are known to occur: The relatively common chorioangioma, and the extremely rare teratoma. Most chorioangioma are small and only detected by careful sectioning of the placenta. Large, externally visible chorioangioma, although uncommon, may result in both maternal and fetal complications. The incidence of chorioangioma is still an unsettled issue varying between one in 3,500 and one in 9,000 placentas because of overlooking at examination of the placenta. Large tumors more than 4 cm in diameter are clinically important. The maternal complication of polyhydramnios, preeclampsia, and premature labor are those described in the chorioangioma syndrome. The neonatal complications include microangiopathic anemia, thrombocytopenia, hymolysis, low birth weight, and neonatal death. In this respect, the importance of ultrasound in the diagnosis of placental chorioangioma has now been recognized. In this paper, a case of large chorioangioma in a viable pregnancy, associated with hydroamnios, preterm labor, is presented with the brief review of the literature related to this type of tumor.

쌍태아 중 무두무심태아 1 례

정주섭(JS Chung),김상국(SK Kim),이환우(HW Lee),김은주(EJ Kim),배국환(KH Bae) 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.1

Acardic twining is a rare congenital anomaly, usually in monozygotic twins. This condition is characterized by the absence or rudimentary development of fetal hear, and associated with various anomaly(especially with anecephalus). The acardiac malformations are uniformly fatal in the affected twin, and mortality in the co-twin is as high as 55%. We recently experienced a case of acardius acephalus associated with a normal term male infant, so present with a brief review of the literature.

중합효소연쇄반응을 이용한 한국인 혈우병 A 환자의 유전자내 BclI 및 XbaI RFLP 양상에 관한 연구

최영민(YM Choi),황도영(DY Hwang),최진(J Choi),구승엽(SY Koo),김석현(SH Kim),신창재(CJ Shin),김정구(JK Kim),문신용(SY Moon),이진용(JY Lee),장윤석(YS Chang),안효섭(HS Ahn),김은주(EJ Kim) 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.12

Hemophilia A is one of the most common inherited bleeding disoders in humans, resulting from thterogenous colletion of mutations in the factor VIII gene. With the molecular clonig of the human gene for factor VIII, intragenic polymorphic DNA sequeces have been described and these sequeces are currently used for accurate identfication of carriers and for prenatal diagnosis, usually by radioactive Southern blot analysis which is costly and labor intensive. The recent development of polymerase chain reaction provides a more rapid and nonradioactive technique for the carrier-detection and prenatal diagnosis. To identify the clinically useful markers that can be used for the carrier-detection and prenatal diagnosis, we analysed the BclI and XbaI intragenic polymorphism of factor VIII gene in 23 Korean patients with hemophilia A and their mothers using polymerase chain reaction(PCR). BclI intragenic polymorphism was informative in 4(17.4%) famlies among 23 families tested, whie XbaI intragenic polymorphism was informative in one (4.3%) family among those familes. In some mothers of the patient, anaylsis of XbaI polymorphism was not possible by digestion because of the background bands that interfere with interpretation. These data indicated that PCR-based analysis of BclI intragenic polymorphism is a clinically useful method for the carrier-detection and for prenatal diagnosis of hemophilia A in Korean patients.

특별한 혈소판 감소성 자반증에 합병된 만삭임신 1 예

한원희(WH Han),노성일(SI Rho),유국영(KY Yoo),노경병(KB No),이훈(H Lee),김은주(EJ Kim) 대한산부인과학회 1987 Obstetrics & Gynecology Science Vol.30 No.1

ITP에 합병된 만삭임신에서 감마글로부린을 정맥주사하여 혈소판 수를 증가시킨 후, 제왕절개술을 성공적으로 시행한 1예를 체험하였기에 이를 문헌 고찰과 함께 보고하는 바이다 Idiopathic thrombocytopenic purpura may cause hemorrhagic complications in pregnant women, and transplacental transfer of IgG platelet autoantibodies often leads to neonatal thrombocytopenia Early splenectomy, corticoids and plasma exchange have all been used to provoke remission, and elective cesarean section is often recommended Infusion of high dose intravenous immunoglobulin is a new measure in treating ITP for reversal of platelet count with little side effect We report our experience with this agent in a pregnant woman with ITP and performed cesarean section safely