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자연산 넙치, Paralichthys olivaceus에 기생한 거머리, Austrobdella sp. 첫 보고 및 거머리 기생에 의한 숙주의 조직병리학적 변화 관찰
김석렬(Seok-Ryel KIM),고상무(Sang-Mu KO),최훈(Hoon CHOI),김대한(Daehan KIM),박정준(Jung Jun PARK) 한국수산해양교육학회 2017 水産海洋敎育硏究 Vol.29 No.5
This study described the morphological ultrastructure of parasite by using the scanning electron microscope and histolopathological characteristics of the host. The parasite was identified as leech, Austrobdella sp. based on molecular analysis and histopathological reaction of the host. We found that seven Paralichthys olivaceus were infected with thirteen Austrobdella sp.: eight (TL 12.13±0.21) on the caudal fin (61.5%), two on the pelvic fin (15.4%) and three (TL 40.2±3.8) on the body (23.1%). The infected region of the fin was lifted as the leeches sucked the area continuously with their posterior suckers, and this brought about destruction of the epidermal layer and inflammation and necrosis of connective tissues of the host. The fin tissue with leeches on showed the hypertrophy and hyperplasia of mucous cell. The epidermal layer of the fin adjacent to a leech’s posterior sucker was pushed to the opposite side and showed a decrease in mucous cell. The karyolysis and pyknosis of nucleus were observed in the epithelial cells and the necrotic region in the epidermal layer. Although these Austrobdella sp. do not cause direct mortality in the host, the injuries they inflicted on the host have a high potential to lead to the second infection.
김석렬(Seok Ryel Kim),키타무라 신이치(Shin Ichi Kitamura),정성주(Sung Ju Jung),강소영(So Young Kang),김흥윤(Heung Yun Kim),고강희(Kang Hee Ko),오명주(Myung Joo Oh) 전남대학교 수산과학연구소 2006 수산과학연구소논문집 Vol.14 No.1
본 연구는 연안 해수 중에 iridovirus의 동태 파악의 일환으로서, 남해안 지역의 양식어와 해수를 채수하여 농축 후 PCR법을 이용하여 바이러스의 분포 여부를 확인하였다. 1999년 7월 신안, 완도, 여수, 남해 및 통영 해수 시료를 농축 후 검사 결과 남해안 일대의 모든 시료에서 iridovirus에 대하여 양성을 나타내었고, 10월 샘플에서도 완도와 여수 연안 해수에서 iridovirus를 검출할 수 있었다. 2000년 1월과 4월 샘플에서는 iridovirus가 검출되지 않았으나, 7월 샘플에서는 샘플 채수 지역모두 iridovirus가 검출되어, 남해안 일대의 해수에 광범위하게 iridovirus가 해수 내에 분포하고 있음을 시사하고 있다. In this study, we were concentrating virus form natural seawater collected from the south coast and were detecting fish pathogenic virus (Iridovirus) using polymerase chain reaction(PCR). In July 1999, all of the concentrated seawater samples collected from Sinan, Wando, Yeosu, Namhea and Tongyong could detect gene of iridovirus. In Oct. 1999 samples, we could also detected iridovirus in seawater samples collected from coast of Wando and Yosu. None of the seawater samples collected from Jan. to Apr. 2000 tested was positive for iridovirus. However all of the seawater samples collected from the south coast in July, 2000 tested iridovirus-positive, suggesting that iridovirus is widely distributed in seawater of the south coast when temperature of seawater is high in the season.
Red Seabream Iridovirus (RSIV)의 가두리양식장내 감염동태
김석렬(Seok Ryel Kim),배종철(Jong Chul Bae),키타무라 신이치(Shin Ichi Kitamura),정성주(Sung Ju Jung),강소영(So Young Kang),김흥윤(Heung Yun Kim),고강희(Kang Hee Ko),오명주(Myung Joo Oh) 전남대학교 수산과학연구소 2006 수산과학연구소논문집 Vol.14 No.2
본 연구는 양식장 환경수 및 사육중인 어체를 대상으로 2002년 5월부터 10월까지 주기적인 샘플링을 실시하여 양식 현장의 돔류에 발생되어지는 RSIV 감염증의 감염특성을 현장관찰, 사육일지의 관리 및 샘플어의 외부관찰, 해부관찰, 조직병리학적관찰, 해수 바이러스 검출, 바이러스배양 및 PCR검출법을 이용하여 확인하였다. 본 연구의 결과 폐사가 일어나기 이전인 봄철에 돌돔으로부터 체내 바이러스의 존재가 확인되었으나 연안해수에서는 검출되지 않았고 또한 어체 내바이러스의 존재가 확인됨에도 불구하고 양식어는 폐사되지 않았다. 그러나 수온 상승기인 8월초부터 폐사가 발생하였으며 폐사한 양식어의 경우 비장 및 신장조직 등 여려 장기에서 바이러스가 검출되었으며 특히 9월부터 10월 말까지 고수온이 장기간 유지 되어지는 조건에서 많은 폐사가 발생되어졌다.또한 참돔보다 돌돔이 RSIV에 감수성이 높은 것으로 나타났으며 RSIV의 연안수를 매개로한 수평적 감염으로 인하여 10월에는 돌돔뿐만 아니라 참돔에서도 감염을 확인할 수 있어서 수평적 감염의 심각성을 확인할 수 있었다. 이에 따라 양식 현장의 각 시기별 점검과 함께 연안수 중의 바이러스 보유점검을 주기적으로 시행한다면 질병의 확산에 대한 예측이 가능하리라 사료된다. Since 1998, Red seabream Iridovirus (RSIV) has been detected every year and has caused economic damages in net-cage cultured red seabream, Pagrus major and striped beakperch(Japanese parrotfish), Oplegnathus fasciatus (Temminck & Schlegel), in southern and western coastal areas of the Korean peninsula. This study was conducted to know epidemiological characteristics of the RSIV infection in net-cage farm. For the purpose, 116 red seabream and 89 striped beakperch were collected three fish farms in Namhae area during May to October in 2002. First diagnosis included field observations, gross and internal observations of fishes, Giemsa stain of the spleen and kidney stamp to identify enlarged cells which is a characteristic of the RSIV infection. In addition, virus culture by cell lines, histopathological studies and polymerase chain reaction (PCR) were conducted. In spring, RSIV was detected from the fish body but not in sea water. Although RSIV was exist in fish body no mortality was recorded. The mortality recorded from the August, and increased in September and October according with increasing of water temperature. During August and October, RSIV was detected not only dead fish but also sea water. Striped beakperch was more susceptible than red seabream against RSIV. RSIV could be transmitted via sea water and caused mortality in October to red seabream which is less susceptible to RSIV.
Aeromonas salmonicida에 감염된 붉은쏨뱅이, Sebastiscus tertius의 조직병리학적 반응
김석렬 ( Seok-ryel Kim ),진영국 ( Young Guk Jin ),박정준 ( Jung Jun Park ) 한국어병학회 2019 한국어병학회지 Vol.32 No.2
As an epidemiological survey, mortality of marbled rockfish, Sebastiscus tertius observed from a fish farm in Gyeongnam province of South Korea. The major macroscopic sign of the diseased fish was severe multifocal dermal ulceration. Histological observation revealed inflammation, necrosis and colonization of bacteria in various tissues (gill, liver, spleen and kidney). Bacteria was isolated from spleen and kidney in moribund and mortality fish. Seven bacterial isolates from the diseased fish were identified as Aeromonas salmonicida subsp. salmonicida using API 20E and 20NE, API 50CH API ZYM system. Under light microscopy, infected marbled rockfish showed the lifting of the lamella epidermal layer, edematous changes and hypertrophy of epithelial cell in the gill filament. The atrophy of the mucosal fold, erythema in the intestine, and the necrosis of hematopoietic tissue and renal tubule cells with karyolysis were observed in the kidney. In this study was demonstrated the histological reaction of red marbled rockfish infected by Aeromonas salmonicida. Furthermore, this is the first account of extensive dermatitis in Sebastiscus tertius due to atypical A. salmonicida infection, which has high potential in aquaculture among native fish species.
김석렬(Seok Ryel Kim),정성주(Sung Ju Jung),강소영(So Young Kang),오명주(Myung Joo Oh) 전남대학교 수산과학연구소 2006 수산과학연구소논문집 Vol.15 No.1
본 연구는 연안 해수 중에 WSSV의 동태 파악의 일환으로서, 서남해안 지역의 양식새우와 연안 해수를 채수하여 농축 후 PCR법을 이용하여 바이러스의 분포 여부를 확인하였다. 1999년 8월 신안과 고창에서 채집된 해수 시료와 양식 새우 모두 WSSV에 대하여 양성을 나타내었고, 고창의 새우양식 호지에서 채집된 망둥어에서도 WSSV에 양성반응을 보였다. 2000년 5월, 7월 및 9월에 신안, 강진 및 순천에서 샘플된 연안 해수와 인근 새우양식장에서 채집된 양식새우에서도 WSSV에 양성반응을 보였으며, 2001년 6월과 8월에 신안에서 샘플된 연안해수와 인근양식장에서 채집된 새우에서도 WSSV에 양성을 보였다. 본 연구에 의해 서남해안 일대의 새우양식장 연안 해수에 광범위하게 WSSV가 상존하며 분포하고 있음과 양식시기 이외에는 토착 갑각류나 망둥어 등의 숙주에 잠복감염 상태로 있음을 시사하고 있다. In this study, we were concentrating virus form natural seawater of near shrimp farms collected in the west and south coast and were detecting white spot syndrome virus (WSSV) from cultured shrimp using polymerase chain reaction (PCR). From Aug. 1999 to Aug. 2001, both of the cultured shrimp and concentrated seawater samples collected from Sinan, Kochang, Kangjin, and Sunchun could detect gene of WSSV. In Aug. 1999 samples, we could also detected WSSV in common brackish goby samples collected from shrimp farm of Kochang. All of the seawater samples and cultured shrimp collected from the west-south coast in 1999-2000 tested WSSV-positive, suggesting that WSSV is widely distributed in seawater of the west-south coast all time of the year.
항원의 투여방법 및 사육환경 변화에 따른 넙치의 특이항체 반응
김위식 ( Wi Sik Kim ),한종석 ( Jong Seok Han ),장민석 ( Min Seok Jang ),서한길 ( Han Gil Seo ),정성주 ( Sung Ju Jung ),박정준 ( Jung Jun Park ),김석렬 ( Seok Ryel Kim ),오명주 ( Myung Joo Oh ) 한국어병학회 2011 한국어병학회지 Vol.24 No.2
The specific antibody response of olive flounder, Paralichthys olivaceus to change in rearing-environmental conditions post immunization with antigen (bovine serum albumin, BSA) and different routes of antigen administration were investigated by enzyme-linked immunosorbent assay (ELISA). To test the effect of routes of antigen administration, flounder were injected intraperioneally or intramuscularlly with 1 mg of BSA. In addition, to test the effect of change in environmental condition post immunization, flounder were injected intraperioneally with 1 mg of antigen, and then were exposed to acute thermal change (the water temperature (WT) was decreased from 21℃ to 15℃ within 30 min and maintained at 15℃ for 3 h), handling (fish were caught and subsequently held out of water for 1 min) or heavy oil (76 g/ 200 L for 2 days). Consequently, there was no significant difference between intraperioneal (IP) and intramuscular (IM) injections except at 10 days post-immunization. With these results, it suggests that both IM and IP injections may be used as route of vaccination. Futhermore, no significant difference was observed in the antibody response among the groups exposed to heavy oil, handling, sudden drop of WT and positive control except at 10 days post-immunization. From these results, it was confirmed that specific antibody response was not affected by the above mentioned rearing-environmental conditions, suggesting that vaccination can be employed at changing rearing-environmental conditions.