http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
이영하,김무송,최영길 충남대학교 자연과학연구소 1984 忠南科學硏究誌 Vol.11 No.2
The protoplast fusion of Rhizopus suinus was studied to develope a new method for strain improvement in Rhizopus species. High yields of protoplasts from 12hr-age of germlings were obtained by using an enzyme system containing Bacillus HD 2 chitosanase and commercial Driselase with 0.5M mannitol as osmotic stabilizer. The optimum temperature for mycelium digestion was 25℃ and the optimum pH was 6.0. The reversion frequency of these protoplasts on minimal and complete agar medium was 0.39 and 0.45%, respectively. In the presence of polyethylene glycol (PEG), intraspecific hybrids were aquired by the protoplast fusion between protoplasts of UV induced auxotrophs. Thirty percent of PEG (pH 6.0) was found to be most effective and the efficiency of PEG was enhanced by the addition of 10-50 mM CaCl_2. The average fusion frequency under optimum condition was 0.42%.