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RISS 인기검색어
- 검색키워드
- 저자 : 금기창 ( Moon Kee Paik (검색결과 3 건)
- 무료
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태반조직의 Nucleases 에 관한 연구 ( 1 ) 사람 태반조직의 Deoxyribonucleases
백문기,황병두,금기창 ( Moon Kee Paik,Byung Doo Hwang,Ki Chang Kum ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.1
The properties of acid deoxyribonuclease purified from human placenta have been investigated, and serial estimation of acid and alkaline deoxyribonuclease in human placenta has been performed. The acid deoxyribonuclease is partially purified forty-fold with specific activity of 240 units/㎎ protein. The acid deoxyribonuclease having an optimal pH around 5 is heat-labile with complete loss of activity at 70 for 10 minutes. The acid deoxyribonuclease is inhibited by Mg^(2+), Mn^(2+), Car and Co^(2+) above 1 mM concentration, suggesting that the enzyme is not dependent on divalent cations. The acid deoxyribonuclease acts on both native and heat-denatured DNA with preference for the former. A gradual decrease in the acid deoxyribonuclease activity occurs with advancing gestation after 20 weeks, the activity at term being about one-fourth of that before 20 weeks. The alkaline deoxyribonuclease activity is maintained untill approximately the 16th week of gestation at which the functioning villi has been established, and thereafter decreases rapidly with no apparent activity about 28 weeks. From the activity changes with gestational age, it is discussed that the acid deoxyribonuclease is related to DNA synthesis of the placenta whereas the alkaline deoxyribonuclease plays an important role in the formation of the functioning villi, suggesting that alkaline deoxyribonuclease is a useful enzyme to evaluate the function of the early placenta.
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白汶基,黃炳斗,李正馥,琴基昌 충남대학교 의과대학 지역사회의학연구소 1978 충남의대잡지 Vol.5 No.1
Properties of alkaline deoxyribonucleases from cytosol and membane fractions of the early human placenta have been investigated. Evidence is indicating that the cytosol deoxyribonuclease (DNase) having an optimal pH around 9 and the plasma membrane bound DNases having an optimal pH around 8 (pH- 8-PM-DNase) and 9. 5 (pH-9.5-PM-DNase), respectively, are distinct enzyme entities. The cytosol DNase, partially purified, is activated by 1mM CO^2+ and the pH-8-PMDNase and the pH-9. 5-PM-DNase are required Mg^2+ and Co2^2+, respectively, for its maximal activity. The cytosol DNase is relatively heat-labile, whereas the plasma membrane bound DNases are heat-labile with some differnce. The above DNases act both on the native DNA and the heat-denatured DNA with preference for the latter. Kinetic analysis shows that the maximal velocity is independent of Co^2+, while Km decreases from 4. 16 × 10 exp(-4) M DNA-p in the absence of Cot^2+ to 9. 4×10 exp(-5) M DNA-p in the presence of Co^2+. From the above result and the fact that the DNA is not only associated with membrane structure, but also membrane-DNA interaction may be relevant to several pathologic process, including aging and carcinogenesis, we discussed that the PH-9-PM-DNase is related to the differenciation of the early placenta.
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初期 胎盤組織의 膜結合 Deoxyribonucleases의 性狀
白汶基,黃炳斗,琴基昌 충남대학교 의과대학 지역사회의학연구소 1977 충남의대잡지 Vol.4 No.2
Some properties of the plasma membrane-bound deoxyribonucleases (DNases) from the early placenta have been investigated. Evidence is indicating that the DNases having an optimal pH around 8 (pH-8-DNase) and 9.5 (pH-9.5-DNase) are distinct enzyme entities. The pH-8-DNase and the pH-9.5-DNase are required Mg^2+ and Co^2+, respectively, for its maximal activivity. The above DNases are heat-labile with some difference and act both on the native and the heat-denatured DNA with preference for the latter. Lipase, phospholipase-C and -D are not successful for the solubilization of the pH--9. 5-DNase from the membrane structure of the early human placenta. The appararent activity of the pH9.5-DNase is not measureable in the normal term placenta having no cytotrophoblast. From the above results and the fact that the DNA is not only associated with the membrane structure, but als othe membrane-DNA interaction may be relevant to several pathologic process, including aging and carcinogenesis, we discussed that the pH-9.5-DNase is related to the differenciation of the early placenta.
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