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상이 (相異) 한 선량의 자외선을 조사한 브로일러 병아리에 있어서 간장 비타민 D 농도의 경시적 변화
장윤환(Y . H . Chiang),강훈석(H . S . Kang),여영수(Y . S . Yeoh),김강수(K . S . Kim),조인호(I . H . Cho),배은경(E . K . Bae) 한국축산학회 1992 한국축산학회지 Vol.34 No.5
This study was carried out to elucidate the time course variation of liver vitamin D(VD) concentration of broiler Hubbard chicks fed VD-deficient diet for 31 days in a subdued light room and exposed to UVB light(maximum intensity at 297 nm) with dose of 0.204 or 0.408 mJ/㎠. The lipid in liver was extracted by chloroform-methanol (2:1) and VD fraction was separated by Sep-Pak silica cartridge. The VD contents were analyzed by normal phase HPLC. The control birds received no UVB irradiation showed the liver VD level of 5 ng/g. When 0.204 mJ/㎠ was treated to whole body of chicks, the liver VD level tended to increase until 90 hrs after irradiation, where the maximum value of 11 ng/g appeared. A decreased level of ng/g was present at 138 hrs after exposure. The peak value (16 ng/g) was shown also at 90 hrs after UVB treatment when 0.408 mJ/㎠ was applied. A low level of 5 ng/g was present at 138 hrs after irradiation. Consequently it was verified that 0.408 mJ/㎠ of UVB treatment produced more VD in liver of broiler chicks than the 0.204 mJ/㎠ did, and the highest level of liver VD appeared at 90 hrs after exposure.
브로일러 병아리의 등 피부에 312 mm 자외선 조사시 상이한 선량이 혈장내 비타민 D3 함량에 미치는 영향
장윤환(Y . H . Chiang),원지웅(J . W . Won),강훈석(H . S . Kang),김강수(K . S . Kim),여영수(Y . S . Yeoh) 한국축산학회 1992 한국축산학회지 Vol.34 No.3
This study was conducted to elucidate the effect of different dose of 312 nm UV light on plasma vitamin D₃(D₃) content of 3 week old broiler chicks. The 0.32, 0.65 or 0.98 J/㎠ of dose was applied on dosal skin of birds and blood samples were collected day 0, 1, 2, 3, 4 and 5 after UV exposure. The plasma was isolated and D₃ concentrations were analyzed by HPLC. When dorsal skin was exposed to 0.32 J/㎠ of UV dose, the mean plasma D₃ content was 24 ng/㎖ immediately after irradiation and 46 ng/㎖ (peak) at day one after exposure. The D₃ level tended to decrease until day 5 (10 ng/㎖). As 0.65 J/㎠ of UV dose was treated, the D₃ concentrations were 20, 22, 37, 14, 29 and 10 ng/㎖ at day 0, 1, 2, 3, 4 and 5 after irradiation, respectively. When 0.98 J/㎠ dose was applied, the plasma D₃ levels were 25, 25 and 34 ng/㎖ at day 0, 1 and 2, respectively and decreased thereafter. Consequently it was shown that the treatment of 0.32 J/㎠ produced more D₃ in chick plasma than 0.65 or 0.98 J/㎠ did (P$lt;.05).