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유전자 재조합 인간 성장호르몬(rhGH)이 NK 세포의 활성에 미치는 영향
강창랑,김종덕,류훈,정헌택 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.4
To evaluate the acute effect of recombinant human growth hormone(rhGH) on the activity of natural killer(NK) cell in children, we selected 6 patients of growth hormone deficient and 5 normal control children. In both groups, 0.15 IU/kg of rhGH was administered subcutaneously. The plasma growth hormone level were measured by radioimmunoassay on 0, 2, and 6 hours after administration of rhGH. To determine NK cell activity, peripheral blood mononuclear cell were separated by Ficoll-Hypaque gradient sedimentation. After this, varying concentration of viable effector cells in complete medium were added to duplicate culture of target cells(the human erythroleukemia cell line, K562) and we measured the LDH released from the cytosol into 16 hours culture medium. The average paasma level of growth hormone of growth hormone deficient and normal group were increased to the level of 41.6±23.7 and 96.3±46.5 ng/ml 2 hours after rhGH injection respectively and decreased to the level of 18.510.6 and 42.2± 5.5 ng/ ml 6 hours after rhGH injection respectively. In 16 hours_ culture medium, percent cytotoxicity of NK cell was significantly increased in the blood samples of control groups. It seemed that administration of the rhGH do not made a any effects acutely on NK cell activity in growth hormone deficient group. But in a normal control children, external administration of growth hormone increased percent cytotoxicity of NK cell activity.
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