C 반응성 단백질이 사람 Macrophage 탐식 기능에 미치는 영향

김용호(Yong-Ho Kim),강신원(Shin-Won Kang) 대한의생명과학회 1998 Biomedical Science Letters Vol.4 No.1

치료목적으로 채취한 사람 복수에서 C 반응성 단백질 (CRP)을 분리 정제하여 사람 대식세포 탐식활성에 미치는 영향에 대하여 연구하였다. CRP는 p-diazonium phenyl-phosphoryl choline 혹은 C-polysaccharide coupled sepharose 4B와 hydroxylapatite affinity chromatography법으로 분리 정제시켰다. 대식세포는 ficoll hypaque 밀도 원심구배법으로 분리시킨 다음 부착법으로 정제시키고 탑식시험을 이용하여 확인하였다. CRP가 대식세포의 시험관내 미생물 탐식활성에 미치는 영향은 촉진 혹은 억제시키는 경향을 보였다. 즉, CRP와 대식세포 미생물 탐식능과의 관계는 반응시킨 시간, 반응계에 가해준 CRP량, 미생물ㆍ탐식세포 및 CRP 상호간 반응시킨 순서에 따라서 다르게 나타났다. 대식세포의 시험관내 탐식활성에 미치는 CRP 자극의 특성은 한계자극 특성을 보였다. The effects of CRP purified from human ascites fluid on phagocytic activity of the human macrophage were investigated. CRP was purified using affinity chromatography including absorption on p-diazonium phosphocholine or C-polysaccharide coupled sepharose 4B and gel filtration on hydroxylapatite column chromatography. Macrophage was separated ficoll hypaque gradient density and absorption method, and then was confirmed phagocytic uptake test using latex method. CRP was able either to inhibit or to enhance phagocytic activity of human macrophage against bacteria in vitro. The effects of CRP on phagocytic activity of human macrophage were in time and dose-dependent manners. The additional sequence of reaction mixture against bacteria in vitro shows a threshold stimulus on the activation of phagocytic response upon the CRP.

Vibrio Vulnificus Lipopolysaccharide의 생물활성과 그에 미치는 Cell Wall Protein-A의 영향

김용호,신홍대,강신원,Kim, Yong-Ho,Shin, Hong-Dae,Kang, Shin-Won 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.3

Vibrio(V.) vulnificus 대량 배양에 의하여 회수된 균체로부터 lipopolysaccharide(LPS)와 cell wall protein-A(CWP-A)를 추출하여 Sprague Dawley rat에서 생물활성을 생체의 독소 배설 기능에 관련된 장기의 효소 및 혈중 성분을 비교 측정한 결과 aspartate transaminase(AST), alanine aminotransferase(ALT) 및 blood urea nitrogen(BUN)은 LPS쪽의 활성이 높았으나 lactic dehydrogenase(LDH)는 CWP-A쪽이 훨씬 높았다. 기타의 측정군은 양자에서 모두 유사한 활성을 나타내었다. 따라서 숙주 혈중 순환 효소 및 성분의 변동 성향을 고려해 볼 때 V. vulnificus 병독성 해석에 내독소인 LPS 뿐만 아니라 CWP-A도 중요하게 고려되어야 한다고 본다. Lipopolysaccaride(LPS) and cell wall protein-A(CWP-A) were extracted from cell wall of Vibrio(V.) vulnificus. The biological activities of CWP-A were weaker than LPS in aspartate transaminase(ASP), alanine aminotransferase(ALT) and blood urea nitrogen(BUN), but more strong in lactic dehydrogenase(LDH) and very similiar in other test. CWP-A of V. vulnificus were considered to be important for the expression of virulence, LPS as well.

Vibrio Vulnificus Lipopolysaccharide 의 생물활성과 그에 미치는 Cell Wall Protein - A 의 영향

김용호,신홍대,강신원 ( Yong Ho Kim,Hong Dae Shin,Shin Won Kang ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.3

Lipopolysaccaride(LPS) and cell wall protein-A(CWP-A) were extracted from cell wall of Vibrio(V.) vulnificus. The biological activities of CWP-A were weaker than LPS in aspartate transaminase(ASP), alanine aminotransferase(ALT) and blood urea nitrogen(BUN), but more strong in lactic dehydrogenase (LDH) and very similiar in other test. CWP-A of V. vulnificus were considered to be important for the expression of virulence, LPS as well.

광기능성 폴리머 도파로형 자외선 센서의 제작 및 특성

김규진 ( Kyu Jin Kim ),장수원 ( Su Won Jang ),강병호 ( Byoung Ho Kang ),김도억 ( Do Eok Kim ),권대혁 ( Dae Hyuk Kwon ),김성훈 ( Sung Hoon Kim ),이용현 ( Yong Hyun Lee ),강신원 ( Shin Won Kang ) 한국센서학회 2006 센서학회지 Vol.15 No.4

Vibrio vulnificus 의 Cell Wall Protein A 와 Lipopolysaccharide ( LPS ) 의 추출 및 이들이 효소활성에 미치는 영향

김용호,이봉헌,박장수,강신원 ( Yong Ho Kim,Bong Hun Lee,Jang Su Park,Shin Won Kang ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.5

Lipopolysaccharide (LPS) and cell wall protein A from Vibrio vulnificus were extracted, were tested the lethal toxicity and Limulus amebocyte gelation activity, and were examined the effect of cell wall protein A on the enzyme activity of LPS. These results were compared to those of LPSs and cell wall protein As from Escherichia coli and Salmonella typhimurium. The lethal toxicity of V vulnificus LPS (LDSO was 138.6 ㎎/㎏) was lower than those of E. coli LPS (56.3 ㎎/㎏) and S. typhimurium LPS (37.5 ㎎/㎏), but the Limulus gelation activities of all LPSs were the same value (0.1 ng/㎖). The results of enzyme activity by LPS and cell wall protein A have shown that the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) were increased. The cell wall protein A on the enzyme activities of LPS was effective; the enzyme activities of LPS-cell wall protein A mixture were greater than those of LPS only.

Vibrio vulnificus의 Cell Wall Protein A와 Lipopolysaccharide (LPS)의 추출 및 이들이 효소활성에 미치는 영향

김용호,이봉헌,박장수,강신원,Kim, Yong-Ho,Lee, Bong-Hun,Park, Jang-Su,Kang, Shin-Won 생화학분자생물학회 1994 한국생화학회지 Vol.27 No.5

Vibrio vulnificus에서 lipopolysaccharide(LPS)와 cell wall protein A를 추출하여 치사독성 및 Limulus겔화 활성을 측정한 후 LPS의 효소활성에 미치는 cell wall protein A의 영향을 조사하였다. 이 결과들을 Escherichia coli와 Salmonella typhimurium LPS 및 cell wall protein A의 것들과 비교하였다. V. vulnificus LPS의 치사독성($LD_{50}$는 138.6 mg/kg)은 E. coli LPS와 S. typhimurium LPS의 것(56.3 mg/kg와 37.5mg/kg)보다 낮았지만 세가지 LPS의 Limulus 활성은 모두 같았다(0.1 ng/ml). LPS와 cell wall protein A에 의한 alanine aminotransferase(ALT), aspartate aminotransferase(AST)와 lactate dehydrogenase(LDH)의 활성은 증가하였다. 한편 LPS의 효소활성에 미치는 cell wall protein A의 영향은 커서 LPS와 cell wall protein A를 혼합한 것의 효소활성이 LPS만의 것보다 컸다. Lipopolysaccharide (LPS) and cell wall protein A from Vibrio vulnificus were extracted, were tested the lethal toxicity and Limulus amebocyte gelation activity, and were examined the effect of cell wall protein A on the enzyme activity of LPS. These results were compared to those of LPSs and cell wall protein As from Escherichia coli and Salmonella typhimurium. The lethal toxicity of V. vulnificus LPS ($LD_{50}$ was 138.6 mg/kg) was lower than those of E. coli LPS (56.3 mg/kg) and S. typhimurium LPS (37.5 mg/kg), but the Limulus gelation activities of all LPSs were the same value (0.1 ng/ml). The results of enzyme activity by LPS and cell wall protein A have shown that the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) were increased. The cell wall protein A on the enzyme activities of LPS was effective; the enzyme activities of LPS-cell wall protein A mixture were greater than those of LPS only.

운전자의 체압 분포 및 시트변형에 대한 정량화 측정시스템

권영은 ( Yeong-eun Kwon ),김윤영 ( Yun-young Kim ),이용구 ( Yong-goo Lee ),이동규 ( Dongkyu Lee ),권오원 ( Ohwon Kwon ),강신원 ( Shin-won Kang ),이강호 ( Kang-ho Lee ) 한국센서학회 2018 센서학회지 Vol.27 No.6

Proper seat design is critical to the safety, comfort, and ergonomics of automotive driver’s seats. To ensure effective seat design, quantitative methods should be used to evaluate the characteristics of automotive seats. This paper presents a system that is capable of simultaneously monitoring body pressure distribution and surface deformation in a textile material. In this study, a textile-based capacitive sensor was used to detect the body pressure distribution in an automotive seat. In addition, a strain gauge sensor was used to detect the degree of curvature deformation due to high-pressure points. The textile-based capacitive sensor was fabricated from the conductive fabric and a polyurethane insulator with a high signal-to-noise ratio. The strain gauge sensor was attached on the guiding film to maximize the effect of its deformation due to bending. Ten pressure sensors were placed symmetrically in the hip area and six strain gauge sensors were distributed on both sides of the seat cushion. A readout circuit monitored the absolute and relative values from the sensors in real-time, and the results were displayed as a color map. Moreover, we verified the proposed system for quantifying the body pressure and fabric deformation by studying 18 participants who performed three predefined postures. The proposed system showed desirable results and is expected to improve seat safety and comfort when applied to the design of various seat types. Moreover, the proposed system will provide analytical criteria in the design and durability testing of automotive seats.

실험동물용 가시광선/근적외선 생체 이미징 소형 장비의 개발

엄년식 ( Nyeon Sik Eum ),박희준 ( Hee Joon Park ),정진용 ( Jin Yong Jung ),한정현 ( Jung Hyun Han ),김형경 ( Hyung Kyung Kim ),장은윤 ( Eun Yoon Jang ),이석재 ( Suck Jae Lee ),강병호 ( Byoung Ho Kang ),강신원 ( Shin Won Kang ) 한국센서학회 2012 센서학회지 Vol.21 No.4

In this study, we have developed a mobile-based visible/NIR(Near InfraRed) imaging equipment for the animal testing. This equipment can provide visible, NIR and merged image through the viewer program. Especially, merged image help researcher to understand visual messages at animal in-vivo test. Also, it is available to send real-time images through the smart phone. Researcher can communicate with another researcher who is a long distance away. Also, the equipment was made with portable small size to enable it to commercialize.

C 반응성 단백질이 사람 Macrophage 탐식 기능에 미치는 영향

김용호,강신원 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1998 Journal of biomedical laboratory sciences Vol.4 No.1

치료목적으로 채취한 사람 복수에서 C 반응성 단백질 (CRP)을 분리 정제하여 사람 대식세포 탐식활성에 미치는 영향에 대하여 연구하였다. CRP는 p-diazonium phenyl-phosphoryl choline혹은 C-polysaccharide coupled sepharose 4B와 hydroxylapatite affinity chromatography법으로 분리정제시켰다. 대식세포는 ficoll hypaque 밀도 원심구배법으로 분리시킨 다음 부착법으로 정제시키고 탐식시험을 이용하여 확인하였다. CRP가 대식세포의 시험관내 미생물 탐식활성에 미치는 영향은 촉진 혹은 억제시키는 경향을 보였다. 즉, CRP와 대식세포 미생물 탐식능과의 관계는 반응시킨 시간, 반응계에 가해준 CRP량, 미생물·탐식세포 및 CRP 상호간 반응시킨 순서에 따라서 다르게 나타났다. 대식세포의 시험관내 탐식활성에 미치는 CRP 자극의 특성은 한계자극 특성을 보였다. The effects of CRP purified from human ascites fluid on phagocytic activity of the human macrophage were investigated. CRP was purified using affinity chromatography including absorption on p-diazonium phosphocholine or C-polysaccharide coupled sepharose 4B and gel filtration on hydroxylapatite column chromatography. Macrophage was separated ficoll hypaque gradient density and absorption method, and then was confirmed phagocytic uptake test using latex method. CRP was able either to inhibit or to enhance phagocytic activity of human macropghage against bacteria in vitro. The effects of CRP on phagocytic activity of human macrophage were in time and dose-dependent manners. The additional sequence of reaction mixture against bacteria in vitro shows a threshold stimulus on the activation of phagocytic response upon the CRP.