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趙玟錫 전북대학교 법학연구소 2002 法學硏究 Vol.23 No.-
The purpose of this study is to suggest solution to the different problem from alienation of real property. Especially, the study deal with the following item in reation with the Jurisprudence of alienation of property right of real estate, specifically with Article §186 of the Civil Code. The original text is studied the basic legal principles of the alienation of the property right of real estate. For it is necessary to clarify the basic principles on the alienation of the property right, and this investigates action preceding the transfer of the property right, that is, the relation between legal processes, which cause the transfer of the property right in the current Korea Civil-law §186.
조민석,박동석,Yeo Hong Yun,김성환,심명용,최창원,김영식 한국식물병리학회 2012 Plant Pathology Journal Vol.28 No.1
For the genetic differentiation of Pseudomonas syringae pathovar tomato, a total of 51 P. syringae pv. strains infecting 33 different host plants were analyzed using repetitive element PCR(REP-PCR) and universal rice primer PCR(URP-PCR). The entire DNA fingerprint profiles were analyzed using unweighted pair-group method with arithmetic averages (UPGMA). The 51 P. syringae pv. strains could be divided into five clusters based on 65% similarity by Rep-PCR using BOX,ERIC, and REP primers. P. syringe pv. tomato cluster was well separated from other 31 P. syringae pathovars. P. syringae pv. tomato cluster included only P. syringae pv. maculicola and P. syringae pv. tomato. P. syringae pv. tomato strains could be divided into two genetic groups. Meanwhile, the Pseudomonas pv. strains could be divided into four clusters based on 63% similarity by URP-PCR using 2F, 9F, and 17R primers. P. syringae pv. tomato cluster was also well separated from 30 other P. syringae pathovars. In this case, P. syringae pv. tomato cluster included P. syringae pv. maculicola, P. syringae pv. berberidi, and P. syringae pv. tomato. P. syringae pv. tomato strains was also separated into two genetic groups by URP-PCR analysis. Overall, our work revealed that P. syringae pv. tomato can be genetically differentiated from other P. syringae pathovars by the DNA fingerprint profiles of REP-PCR and URP-PCR. We first report that there are two genetically diverged groups in P. syringae pv. tomato strains.
조민석,박덕환,남궁민,안태영,박동석 한국식물병리학회 2015 Plant Pathology Journal Vol.31 No.2
Clavibacter michiganensis subsp. sepedonicus (Cms) multiplies very rapidly, passing through the vascular strands and into the stems and petioles of a diseased potato. Therefore, the rapid and specific detection of this pathogen is highly important for the effective control of the pathogen. Although several PCR assays have been developed for detection, they cannot afford specific detection of Cms. Therefore, in this study, a computational genome analysis was performed to compare the sequenced genomes of the C. michiganensis subspecies and to identify an appropriate gene for the development of a subspecies-specific PCR primer set (Cms89F/ R). The specificity of the primer set based on the putative phage-related protein was evaluated using genomic DNA from seven isolates of Cms and 27 other reference strains. The Cms89F/R primer set was more specific and sensitive than the existing assays in detecting Cms in in vitro using Cms cells and its genomic DNA. This assay was also able to detect at least 1.47×102 copies/μl of cloned-amplified target DNA, 5 fg of DNA using genomic DNA or 10-6 dilution point of 0.12 at OD600 units of cells per reaction using a calibrated cell suspension.