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장영석(Young Seok Jang),민경수(Kyung Soo Min),오용비(Yong Bee Oh),정동희(Dong Hee Chung) 한국육종학회 1997 한국육종학회지 Vol.29 No.4
Microspore culture was carried out in order to compare the difference of embryo yield between spring type and winter type of Brassica napus L. and isolated microspores from three genotypes of winter type and three genotypes of spring type were cultured in modified Nitsch and Nitsch (NN) medium supplemented with 13% (w/v) sucrose, 0.05mg/ℓ BA and 1.0mg/ ℓ NAA. Temperature was 32.5℃ for 3 days of the initial stage in the dark and maintained at 25℃ in the dark. Embryo yields and frequency of normal embryo production showed remarkable contrast according to genotypes and growth habit. The highest frequency of embryogenesis was obtained from buds of 2.8~3.2mm in size approximately one week after first flowering and the buds of the sizes contained mainly microspores of late uninucleate stage. Embryos were induced from microspores isolated from the buds of 2.3~2.7mm, 2.8~3.2mm, 3.3~3.7mm and 3.8~4.2mm in the spring type. But only the buds of 2.3~2.7mm and 2.8~3.2mm were able to from embryos in the winter type.
Brassica 種들의 花뢰크기에 의한 小胞子 發育段階 및 胚發生 比較
장영석(Young Seok Jang),민경수(Kyung Soo Min),오용비(Yong Bee Oh),정동희(Dong Hee Chung) 한국육종학회 1997 한국육종학회지 Vol.29 No.4
The purpose of this study is to search for the identifing method that could separate buds with embryogenic potential from collected buds for increasing the efficiency of embryogenesis by microspore culture in Brassica species. It was investigated developmental stage of microspore by bud-size and ratio of petal/anther length and compared to amount of embryogenesis through isolated microspore culture from the buds with late-uninucleate stage. In Brasica napus, Lisandra of spring type showed bud-size of 3.2~4.1mm which belongs to late-uninucleate stage as embryogenic microspores and their range of petal/ anther length ratio were 1/2~3/4. Hallayuchae of winter type rapeseed showed bud-size of 3.1~3.6mm which belongs to late-uninucleate stage as embryogenic microspores and their range of petal/anther length ratio were 1/3~1/2. Two genotypes of B juncea had the same petal/anther length ratio as range of 1/2~3/4 with embryogenic microspores but their bud-size were some difference as bud-size of Young-sanpohwanggyeja were 2.9~4.0mm and bud-size of Hwanggyeja were 2.9~4.1mm. Also, two genotypes of B. campestris had the same petal/anther length ratio as range of 2/3~3/4 with embryogenic microspores but bud-sizes were some difference as bud-size of Hyakusai were 2.9~3.4mm and bud-size of Sosongchae were 2.9~3.2mm. In B. oleracea, the petal/anther length ratio with embryogenic microspores according to bud-size were showed range of 1/2~2/3 in all of two genotypes and bud-size of Broccoli were 2.4~3.2mm and bud-size of Cauliflower were 2.9~3.7mm. In the embryo yield per 100,000 microspores by isolated microspore culture from the buds with embryogenic microspores, Young-sanpohwanggyeja of B. juncea were 47 embryos and Hwanggyeja of B. juncea were 23 embryos, B. campestris of Hyakusai were 367 and Sosongchae were 123, B. oleracea of Broccoli were 678 and Cauliflower were 562. However in B. napus was showed much differences by the growth habit, the spring type of Lisandra were producted 7,047 embryos of which higher about 9.2 times than those of winter type Hallayuchae.
노용덕(Yong Deok Noh),장영석(Young Seok Jang),차상일(Sang Il Cha),정문기(Mun Ki Chung),김성구(Sung Goo Kim) 한국정보과학회 2008 정보과학회 컴퓨팅의 실제 논문지 Vol.14 No.9
기존의 웹을 통한 에듀테인먼트 게임이 갖는 문제점 중 하나는 제한된 콘텐츠와 더불어 그 틀이 고정되어 있다는 점이다. 이러한 게임구조하에서는 개개인마다 다른 개인적 취향을 제대로 살리지 못해 게임을 통한 학습의 능률이 제한적이라는 단점이 있다. 이러한 문제를 극복하고자 본 논문에서는 웹 2.0을 기반해 사용자가 관심을 가지는 학습 콘텐츠를 선택할 뿐 아니라 직접 게임의 틀을 선택할 수 있는, UCG(User Combinative Game)이라고 명명한 시스템을 제안하고자 한다. 여기서는 UCG 시스템의 구성과 작동 프로세스를 보인다. One of drawbacks of the current edutainment game system is that its frame is fixed with limited contents. Under the condition of the above game structures, nobody can select one's interesting contents to increase educational efficiency easily. To overcome such a problem, we will propose the system named UCG(User Combinative Game) based on Web 2.0 in which users can make a choice among various educational contents and select game frames. The structure of the UCG system and its process are shown in this paper.