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십자화과 식물의 나출원형질체의 단리와 그 배양에 관한 연구
金映來,李英馥,咸麟基,朴敎善 충남대학교 농업과학연구소 1986 農業技術硏究報告 Vol.13 No.2
The study was carried out to identify several factors affecting isolation and culture of cotyledone and leaf mesophyll protoplasts of cabbage (Brassica oleraceae), petsai (B. campestris subsp. pekinensis) and rape (B. napus). High viable protoplasts could be obtained when the cotyledon and the leaf mesophyll tissue of all species were treated with enzyme solution composed of 1% macerozyme 'R-10', 1.5% Onozuka 'R-10', 10 %mannitol and 50,0mM CaCl_22H_2O for 4 hours. The protoplasts which obtained from the cotyledon of all species except the cabbage and the leaf mesophyll tissue of all species were divided on NN culture medium supplemented with 9.1% mannitol, 1% glucose, 1% sucrose, 1 mg/ℓ 2,4 -D, 0.5mg/ℓ NAA and 0.5mg/ℓ BA. The division of the rape leaf mesophyll protoplasts were continued and led to colony.