http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Lee-Stadelmann, Ok Young,Lee, Seungwoo,Chung, Haejoon,Guo, Quansheng,Kim, Myungwon,Pak, Chunho,Hackett, Wesley P. 경희대학교 유전공학연구소 1991 遺傳工學論文集 Vol.3 No.-
In vitro adventitious bud formation was investigated in 3 hybrid Populus clones with varying degrees of regeneration potential. Longitudinal and transverse wounding treatment of 1.0 cm leaf midvein and petiole explants both increased the shoot regneration capacity, more effectively with transverse wounding. Use of micro-cross sections (400 um in thickness) of leaf midvein or stem internode greatly improved regeneration capacity in all 3 clones. Supplementary calcium nitrate (7.05 to 9.4 mM) with optimal BA and NAA concentrations enhanced regeneration potential of micro- cross sections of 2 clones, but not of the most recalcitrant clone. Micro-cross section are excellent explant for obtaining large numbers of uniform adventitious shoots from minimum amount of material in a short regeneration time.
Agrobacterium rhizogenes 를 이용한 Populus tremuloides 의 형질전환
류기중(Key Zung Riu),소인섭(In Sup So),유장걸(Zang Kual U),고영환(Yong Hwan Ko),이선주(Sun Joo Lee),(Wesley P . Hackett) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.2
Several factors affecting on transformation efficiency were studied to establish a Agrobacterium rhizogenes mediated system for the transformation of Populus species, and We could obtaine tansgenic plantlets expressing the introduced gene. Leaf sections were more sensitive than stem sections to kanamycin and thought to be better material for transformant screening. The bacterial density did not affect on the efficiency of transformation over the range of 4×10^5∼7×10^9 cfu. The optimum period for co-cultivation was one day or shorter. Both of the optimum concentrations of cefotaxime and ampicillin in the medium were 250 ㎍/㎖ for elimination of bacteria from the inoculated leaf sections. The addition of acetosyringone in the bacterial culture medium increased transformation rate, and the highest rate was obtained at 50 μM of acetosyringone. The transformed galls could be selectively induced and gown on the growth regulator-free medium or on the medium containing 100 ㎍/㎖ or higher contrition of kanamycin. The roots were induced from the galls incited by A rhizogenes within 3 weeks on the growth regulator-free medium as well as on the medium containing growth regulators. The plantlets were regenerated from the galls cultured for 6 weeks on the medium containing 0.05 ㎎/㎖ of NAA and 0.5 ㎎/㎖ of BA. The expressions of the introduced opine gene in the transformed galls and plantlets were confirmed by the analysis of agropine and mannopine.