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강현숙,김백윤,박미진,신영호 한국스포츠리서치 2005 한국 스포츠 리서치 Vol.16 No.2
The purpose of this study was to explore the effects of Taichichuan training on the variables of cognitive-affective states. The subjects of the study consisted of 22G middle aged women in Jeonju areas. The survey methods of this research are the 5 Likert Scale modified the Cognitive Affective States Scale by Yang. Myung-Hwan(1998). The used statistical methods for the data analysis were M. SD. Paired sample T-Test. The results obtained by the appropriate analytic processes were followed. 1. In hedonic enjoyment, Taichichuan training women were lower than non-activity women. 2. In feeling of flow, Taichichuan training women were higher than non-activity women. 3. In self-realization, Taichichuan training women were higher than non-activity women. 4. In self-confidence, Taichichuan training women were higher than non-activity women.
Improvement of agricultural traits of SMV-resistant and drought-tolerant soybean transgenic plants
Hye Jeong Kim,Jung Hun Pak,Mi-Jin Kim,Ha Neui Hong,Quyen Nguyen Thi,Yeong Hoon Lee,Ki Hyun Ryu,Kook-Hyung Kim,Soo Young Kim,Young Soo Chung 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Korean soybean variety Kwangan was transformed with coat protein (CP), helper component-proteinase (HC-Pro), and ABRE binding factor 3 (ABF3) genes using highly efficient soybean transformation system. Among these genes, CP and HC-Pro were transformed using RNAi technology. Transgenic plants with CP were confirmed for gene introduction and their expression using PCR, real-time PCR, RT-PCR, Southern blot, and Northern blot. To investigate the response of viral infection with CP, T1 plants were inoculated with SMV-infected leaves and confirmed the existence of mosaic symptom in both leaves and seeds. Two transgenic lines with CP were highly resistant to SMV with clear leaves and seeds while SMV-susceptible lines showed mosaic symptom with seed mottling. The transcript levels of T1 plants with CP were also determined by northern blot, suggesting that SMV-resistant T1 plants did not show viral RNA expression whereas SMV-susceptible T1 plants showed viral RNA expression. Currently, the response of viral infection with HC-Pro is investigating to produce SMV-resistant soybean transgenic plants, and the physiological experiment with ABF3 is also carrying out to produce drought-tolerant soybean transgenic plants.
Mi-Jin Kim,Jung Hun Pak,Hye Jeong Kim,Ha Neui Hong,Quyen Nguyen Thi,Dong Hee Lee,Soon chun Jeong,Young Soo Chung 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Korean soybean variety Kwangan was transformed with ORE7 gene using highly efficient soybean transformation system. The gene is known to exhibit a delayed leaf senescence phenotype in Arabidopsis. To confirm phenotypic characterization of leaf senescence for non-transgenic (NT) and transgenic plants, we transplanted T1 transgenic lines 7, 9, 14 and 15 together with two negative controls (NT and EV) in greenhouse. As a result, line 15 showed dramatic phenotypic characterization of yield increase and senescence delay. In addition, to investigate the agriculture traits for transgenic plants with leaf senescence delaying, T2 transgenic lines and two negative controls were transplanted on GMO fields in Ochang and harvested T3 seeds (2010). Most transgenic lines showed higher total seed weigh than NT. Especially, total seed weight of line 15 was increased by about 180% and 120% compared with the NT and EV, respectively. Therefore, we carried out the second field experiments with T3 transgenic line 15 and NT in Ochang (2011). A total of 117 transgenic plants were divided into two groups, senescence delaying (64 out of 117 plants) and increased yield (53 out of 117 plants), by transcript level of ORE7 gene. Interestingly, among increased yield plants, total seed weight of each 7 plants were increased by more than 200% compared with NT.
Improved resistance of soybean mosaic virus with Coat protein and HC-Pro genes using RNAi method
Hye Jeong Kim,Jung Hun Pak,Mi Jin Kim,Su Yeong Yun,Yeong Hoon Lee,Ki Hyun Ryu,Kook-Hyung Kim,Young Soo Chung 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Soybean mosaic virus (SMV), a member of Potyviridae family, is one of the most typical viral diseases and results in yield and quality loss of cultivated soybean. Due to the depletion of genetic resources for resistance breeding, a trial of genetic transformation to improve disease resistance has been performed by introducing SMV-CP and HC-Pro gene by RNA interference (RNAi) method via Agrobacterium-mediated transformation. Transgenic plants were infected with SMV strain G5 and investigated the viral response. As a result, two lines (3 and 4) of SMV-CP(RNAi) transgenic plants and three lines (2, 5 and 6) of HC-Pro(RNAi) transgenic plants showed viral resistance. In genomic Southern blot analysis, most of lines contained at least one T-DNA insertion in both SMV-CP(RNAi) and HC-Pro(RNAi) transgenic plants. Subsequent investigation confirmed that no viral CP and HC-Pro gene expression was detected in two SMV-resistant lines of SMV-CP(RNAi) and three lines of HC-Pro(RNAi) transgenic plants, respectively. On the other hand, non-transgenic plants and other lines showed viral RNA expression. Viral symptoms affected seed morphology, and clean seeds were harvested from SMV-resistant line of SMV-CP(RNAi) and HC-Pro(RNAi) transgenic plants. In addition, strong viral gene expression was detected from seeds of SMV-susceptible non-transgenic plants and SMV-susceptible transgenic lines. When compared the viral resistance between SMV-CP(RNAi) and HC-Pro(RNAi) transgenic plants, soybean transgenic plants with the HC-Pro gene using RNAi strategy showed much stronger and higher frequency of viral resistance.
Phylogenetic Analysis of Common Millet (Panicum miliaceum L.) using NTS of 5S Ribosomal DNA
Jung Hun Pak,Mi Jin Kim,Hye Jeong Kim,Sang Hyun Shin,Myung-Chul Seo,In-Seok Oh,Ki Young Kim,Tae Wook Jung,Young Soo Chung 한국육종학회 2012 한국육종학회지 Vol.44 No.4
Twenty two common millet (Panicum miliaceum L.) varieties collected from Korea, China and Russia were investigated for their phylogenetic relationship using 5S ribosomal DNA sequences with a hope to provide the basic information on their exact origin. Sequences of 5S rDNA were isolated by PCR. The primers, 5s-rRNA1 and 5s-rRNA2, were designed to isolate the complete NTS. Genomic DNA amplification produced two fragments with different length, 900 bp and 400 bp fragments, confirming the presence of two types of 5S rDNA repeats that differed from each other in the length of the NTS region. Amplified DNAs of 400 bp fragment were subcloned and used for further investigation. The obtained NTS sequences ranged from 200 to 300 bp and homology of sequences among plant materials was much higher than long repeat. CLUSTALW multiple aligment of 5S rDNA sequences from 22 different common millets revealed the clear difference by their origin. And critically different areas with insert or deletion were also confirmed. Those sequence difference seems to be used for discrimination of cultivars from different origin and use as molecular markers for origin identification. In phylogenic tree construction, the clear classification was shown where the genotypes from China and Russia is positioned together and stay away from domestic genotypes.
Flanking sequence analysis of soybean transgenic plants with three recombinant cry1Ac genes
Jung Hun Pak,Mi Jin Kim,Hye Jeong Kim,Su Yeong Yun,Young Soo Chung 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Bacillus thuringiensis(Bt) crystal protein (Cry1Ac) genes encode insecticidal δ-endotoxins that are widely used for the development of insect-resistant crops. Common soybean is a crop of economic and nutritious importance in many parts of the world. Korea soybean variety Kwangan was transformed with Bacillus thuringiensis(Bt) crystal protein genes. We transformed three difference Cry1Ac (Cry1Ac and two modified Cry1Ac) genes into Kwangan using highly efficient soybean transformation system. Transgenic plants with Bt crystal protein genes were confirmed for gene introduction and their expression using PCR, real-time PCR, and RT-PCR. We generated 30 independent lines of transgenic soybean plants. Analysis of the flanking sequences isolated by Inverse PCR revealed complex T-DNA insertion patterns and preferential integration of T-DNA into the intergenic spacer region of the soybean genome. We found 5 different intergenic transgenic soybean lines of soybean genome. Currently, the confirmation of stable gene introduction with Bt genes is also performing by southern blot analysis, physiology test, and agronomic characters are investigating.