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- 저자 : ( Yongqiang Tian ) (검색결과 4 건)
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Yingliang Tian,Wencai Liu,Yongqiang Lu,Shibing Sun 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.5
La1-xSrxMnO3-α(LSMO) and LaMnO3+α(LMO) nanoparticle catalysts have been synthesized via a one-step molten salt route. It was found that the partial substitution of lanthanum by strontium had a promoting effect on the catalytic performance for toluene oxidation. Under the condition of toluene concentration=1000 ppm, toluene/O2 molar ratio=1/200 and the space velocity=20000 mL/(g h), the temperature required for 50% and 90% toluene combustion conversion was 150℃ and 205℃ over LSMO catalyst, respectively. It is concluded that the oxygen vacancy, the molar ratio Mn4+/Mn3+ on the surface and the specific surface area contribute to the improved catalytic performance of the LSMO nanoparticle materials via a one-step molten salt method.
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( Shiting Wang ),( Zhigang Yang ),( Zhenjiang Li ),( Yongqiang Tian ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.7
Microbial transglutaminases (MTGs) are widely used in the food industry. In this study, the MTG gene of Streptomyces sp. TYQ1024 was cloned and expressed in a food-grade bacterial strain, Bacillus subtilis SCK6. Extracellular activity of the MTG after codon and signal peptide (SP Ync M) optimization was 20 times that of the pre-optimized enzyme. After purification, the molecular weight of the MTG was 38 kDa and the specific activity was 63.75 U/mg. The optimal temperature and pH for the recombinant MTG activity were 50℃ and 8.0, respectively. MTG activity increased 1.42-fold in the presence of β-ME and 1.6-fold in the presence of DTT. Moreover, 18% sodium chloride still resulted in 83% enzyme activity, which showed good salt tolerance. Cross-linking gelatin with the MTG increased the strength of gelatin 1.67 times and increased the thermal denaturation temperature from 61.8 to 75.8℃. The MTG also significantly increased the strength and thermal stability of gelatin. These characteristics demonstrated the huge commercial potential of MTG, such as for applications in salted protein foods.
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( Xiaoguang Li ),( Qian Zhang ),( Longzhan Gan ),( Guangyang Jiang ),( Yongqiang Tian ),( Bi Shi ) 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.1
This study is the first report on production and characterization of the enzyme from an Ornithinibacillus species. A 4.2-fold increase in the extracellular protease (called L9<sup>T</sup>) production from Ornithinibacillus caprae L9<sup>T</sup> was achieved through the one-factor-at-a-time approach and response surface methodological optimization. L9T protease exhibited a unique protein band with a mass of 25.9 kDa upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This novel protease was active over a range of pH (4-13), temperatures (30-80℃) and salt concentrations (0-220 g/l), with the maximal activity observed at pH 7, 70℃ and 20 g/l NaCl. Proteolytic activity was upgraded in the presence of Ag<sup>+</sup>, Ca<sup>2+</sup> and Sr<sup>2+</sup>, but was totally suppressed by 5 mM phenylmethylsulfonyl fluoride, which suggests that this enzyme belongs to the serine protease family. L9<sup>T</sup> protease was resistant to certain common organic solvents and surfactants; particularly, 5 mM Tween 20 and Tween 80 improved the activity by 63 and 15%, respectively. More importantly, L9<sup>T</sup> protease was found to be effective in dehairing of goatskins, cowhides and rabbit-skins without damaging the collagen fibers. These properties confirm the feasibility of L9<sup>T</sup> protease in industrial applications, especially in leather processing.
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Chengnan Li,Yingying Gan,Zongshao Li,Mengjing Fu,Yuzhen Li,Xinran Peng,Yongqiang Yang,Guo‑bao Tian,Yi Yan Yang,Peiyan Yuan,Xin Ding 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00
Background P. aeruginosa, a highly virulent Gram-negative bacterium, can cause severe nosocomial infections, and it has developed resistance against most antibiotics. New therapeutic strategies are urgently needed to treat such bacterial infection and reduce its toxicity caused by endotoxin (lipopolysaccharide, LPS). Neutrophils have been proven to be able to target inflammation site and neutrophil membrane receptors such as Toll-like receptor-4 (TLR4) and CD14, and exhibit specific affinity to LPS. However, antibacterial delivery system based on the unique properties of neutrophils has not been reported. Methods A neutrophil-inspired antibacterial delivery system for targeted photothermal treatment, stimuli-responsive antibiotic release and endotoxin neutralization is reported in this study. Specifically, the photothermal reagent indocyanine green (ICG) and antibiotic rifampicin (RIF) are co-loaded into poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NP-ICG/RIF), followed by coating with neutrophil membrane to obtain antibacterial delivery system (NM-NP-ICG/RIF). The inflammation targeting properties, synergistic antibacterial activity of photothermal therapy and antibiotic treatment, and endotoxin neutralization have been studied in vitro. A P. aeruginosa-induced murine skin abscess infection model has been used to evaluate the therapeutic efficacy of the NM-NP-ICG/RIF. Results Once irradiated by near-infrared lasers, the heat generated by NP-ICG/RIF triggers the release of RIF and ICG, resulting in a synergistic chemo-photothermal antibacterial effect against P. aeruginosa (~ 99.99% killing efficiency in 5 min). After coating with neutrophil-like cell membrane vesicles (NMVs), the nanoparticles (NM-NP-ICG/RIF) specifically bind to inflammatory vascular endothelial cells in infectious site, endowing the nanoparticles with an infection microenvironment targeting function to enhance retention time. Importantly, it is discovered for the first time that NMVs-coated nanoparticles are able to neutralize endotoxins. The P. aeruginosa murine skin abscess infection model further demonstrates the in vivo therapeutic efficacy of NM-NP-ICG/RIF. Conclusion The neutrophil-inspired antibacterial delivery system (NM-NP-ICG/RIF) is capable of targeting infection microenvironment, neutralizing endotoxin, and eradicating bacteria through a synergistic effect of photothermal therapy and antibiotic treatment. This drug delivery system made from FDA-approved compounds provides a promising approach to fighting against hard-to-treat bacterial infections.
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