Isolation and Transcriptional Expression of CuZn Superoxide Dismutase from Codonopsis lanceolata

Lee,Kang,In,Jun-Gyo,Yu,Chang-Yeon,Yun,Song-Joong,Min,Byung-Hoon,Rho,Yeong-Deok,Kim,Moo-Sung,Yang,Deok-Chun 한국자원식물학회 2004 Plant Resources Vol.7 No.3

To investigate the defense mechanism against the abiotic stress, a cDNA clone encoding a CuZn superoxide dismutase (CuZnSOD) protein was isolated from a cDNA library prepared from tabroot mRNAs of Codonopsis lanceolata. The eDNA, designated ClSODCc, is 799 nucleotides long and has an open reading frame of 459 bp with a deduced amino acid sequence of 152 residues. The deduced amino acid sequence of ClSODCc matched to the previously reported CuZnSODs. Consensus amino acid residues (His-45, -47, -62, -70, -79, -119 and Asp-82) were involved in Cu-, Cu/Zn-, and Zn- binding ligands. The deduced amino acid sequence of ClSODCc showed high homologies (82%-86%) regardless of species. Expression of ClSODCc by oxidative stress was increased up to 1 h after treatment and declined gradually. Much earlier and stronger expression of ClSODCc was observed in the cold stress treatment.

인삼엽에서 superoxide dismutase Activity 측정 및 Isozyme pattern 검정

양덕조, 김명식, 이성종 忠北大學校部設基礎科學硏究所 1987 自然科學硏究 Vol.1 No.1

-본문참고-

V.I.S.의 槪念 및 製作에 關한 考察 : Concentrated of the V.I.S. Manual of Samchok National University 三陟大學校의 V.I.S. Manual을 中心으로

李允洙,全洋德 三陟大學校 1992 論文集 Vol.25 No.-

Beginning with the industrial revolution in England, spreaded rapidly into European countries, the design movement, new artistic concept, faced with a new phase because of more developed industrial technique and international age. Identity concept in design, with industrial diversity, specialization, internationalization, resulted from the work called 'Visual Identification Standards.' Samchok National University, elevated into four-years' academic system in 1991, has taken a new opportunity of making progress. Keeping pace with the new image, the introduction to Visual Identification Standard is followed necessarily according to the current trend. The V.I.S. Manual has been attempted by general concept. Some problems on the overall plan, design process, and management in the Manual are as follows; 1. It has not gone through the broad convergence of opinions. That is, the identity n ideal system has not ben established. But the Visual Standard of identity was committed. 2. The Visual Identification Standard has been published into a Manual. But it has been used missunderstandingly, or subjectivelly. 3. For the unified image, it should be widely used, and the visual unfamiliarization should be eliminated. Thus, V.I.S. Manual of Samchok National University should be sufficed gradually according to the various phases. In a broad view, the re-design concept should be examined and thoroughly.

Agrobacterium tumefaciens C58에 依하여 形質轉換된 人蔘組織에서의 saponin 生産

梁德春,李政明 경희대학교 부설 식량자원개발연구소 1988 硏究論文集 Vol.9 No.-

The present study was conducted to obtain some basic information on the transformation of ginseng tissue, identification of opine compound and protein, and saponin production from ginseng tissue transformed with pTiC58. Ginseng tumor callus induced by pTiC58 could be continuously cultured on the phytohormone-free basic medium. The transformation was reconfirmed by the detection and identification of opine compounds from the tumor callus. The transformed ginseng calli contained higher amount of protein than normal calli and the protein pattern of transformed calli was quite different from that of normal callus. Higher saponin content was found in the transformed callus than the normal callus, and ginsenoside composition of the transformed callus was similar to that of cultivated ginseng root, but quite different from that of normal callus.

Ti-plasmid에 依하여 形質轉換된 煙草組織의 特性과 再分化 樣相

梁德春,李政明 경희대학교 부설 식량자원개발연구소 1987 硏究論文集 Vol.8 No.-

Studies were conducted to examine the characteristics of teratoma shoots obtained from diploid and haploid tobacco crown gall tumor tissues induced by the inoculation of Agrobacterium tumefaciens and the effects of plant hormones and active carbon in the cultural media on the growth and regeneration patterns of the teratoma shoots. Results can be summarized as follows. 1. Teratoma shoots could be obtained when the crown gall tumor, induced by the inoculation of A. tumefuciens strain C-58 to the stem, was successively grown on hormone-free medium. 2. The growth of tumor callus induced by C-58 strain was inhibited when several plant hormones were added to the cultural media. Plant regeneration could not be obtained from the tumor callus or the teratomas. 3. Addition of active carbon and benzyl adenine to cultural media was effective for shoot elongation and reduction in multiple shoot formation. 4. The growth of tumor callus obtained from the haploid plant was promoted by the addition of plant hormones to the media. Whole plants were also regenerated from the teratoma shoots formed from tumor callus. 5. Normal or transformed plants were regenerated from the tumor callus formed by the inoculation of a strain of A. tumefaciens collected from field soil. The transformed plants are characterized by having twisted dark-green leaves, shorter internodes, and abundant axillary shoots.

만성폐쇄성폐질환의 치료에서 서방형 Theophylline 의 1 일 1 회 제형과 1 일 2 회 제형의 비교

이양덕(Yang Deok Lee),이흥범(Heung Bum Lee),이용철(Yong Chul Lee),이양근(Yang Keun Rhee) 대한내과학회 2002 대한내과학회지 Vol.62 No.2

Background: Theophylline is a weak bronchodilator with a narrow therapeutic window. Much of its clinical benefit derives from effects other than bronchodilaton; therapeutic doses of theophylline increase ventilatory drive, enhance diaphragmatic contractility and increase cardiac output. About 20% of COPD patients respond to theophylline with improved airflow, exercise tolerance and quality of life. Generally it is prescribed as a twice-daily equal-dose regimen so as to produce nearly constant blood level throughout each 24 hour. We compared 12-hour and 24-hour sustained-release theophyllines in the management of chronic obstructive pulmonary disease. Methods: A crossover study was conducted with 27 adult chronic obstructive pulmonary disease requiring daily bronchodilator therapy. The study group included nineteen males and eight females ranging in age from 48 to 71 years. The overall approach was to place the patients first on the twice-daily preparation (Etheophyl?) for 28 days at 8 AM and 8 PM and pulmonary function tests and theophylline level were measured on the 28th day. The patients were subsequently switched to the once-daily preparation (Uniphyl?) in the same daily dose at 8 PM on the 29th day and the same parameters were measured on the 56th day. Results : The mean serum levels of theophylline were 8.80±2.64 μg/mL in the Etheophyl?- treated and 8.48±2.08 μg/mL in the Uniphyl?-treated period. And the FEV1 showed 63.72±8.63% in the Etheophyl?-treated and 62.81±9.81% in the Uniphyl?-treated period. Conclusion: The results suggested that there was little clinical differences between the two medications. The two drugs were equally effective in clinically controlling COPD over the four weeks of treatment.(Korean J Med 62:182-186, 2002)

The analysis of 3-Level Charge Pumping in SOHOS Flash Memory

Yang, Seung Dong,Yun, Ho Jin,Jeong, Kwang Seok,Kim, Yu Mi,Lee, Sang Youl,Oh, Jae Sub,Lee, Hi Deok,Lee, Ga Won Trans Tech Publications, Ltd. 2013 Advanced materials research Vol.658 No.-

<P>This paper discusses the 3-level charge pumping method in planar-type Silicon-Oxide-High-k-Oxid e-Silicon (SOHOS) and Silicon-Oxide-Nitride-Oxide-Silicon (SONOS) devices to find out the reason for degradation of data retention properties. In the CP thechnique, a pulse is applied to the gate of the MOSFET which alternately fills the traps withe electrons and holes, thereby causing a recombination current Icp to flow in the substrate. A 3-level charge pumping method may be used to determine not only interface trap densities but also capture cross sections as a function of trap energy. By applying this method, SOHOS device found to have a higher interface trap density than SONOS device. Therefore, degradation of data retention characteristics is attributed to the many interface trap sites.</P>

Pillar Type Silicon-Oxide-Nitride-Oxide-Silicon Flash Memory Cells with Modulated Tunneling Oxide

Lee, Sang-Youl,Yang, Seung-Dong,Yun, Ho-Jin,Jeong, Kwang-Seok,Kim, Yu-Mi,Kim, Seong-Hyeon,Lee, Hi-Deok,Lee, Ga-Won,Oh, Jae-Sub The Korean Institute of Electrical and Electronic 2013 Transactions on Electrical and Electronic Material Vol.14 No.5

In this paper, we fabricated 3D pillar type silicon-oxide-nitride-oxide-silicon (SONOS) devices for high density flash applications. To solve the limitation between erase speed and data retention of the conventional SONOS devices, bandgap-engineered (BE) tunneling oxide of oxide-nitride-oxide configuration is integrated with the 3D structure. In addition, the tunneling oxide is modulated by another method of $N_2$ ion implantation ($N_2$ I/I). The measured data shows that the BE-SONOS device has better electrical characteristics, such as a lower threshold voltage ($V_{\tau}$) of 0.13 V, and a higher $g_{m.max}$ of 18.6 ${\mu}A/V$ and mobility of 27.02 $cm^2/Vs$ than the conventional and $N_2$ I/I SONOS devices. Memory characteristics show that the modulated tunneling oxide devices have fast erase speed. Among the devices, the BE-SONOS device has faster program/erase (P/E) speed, and more stable endurance characteristics, than conventional and $N_2$ I/I devices. From the flicker noise analysis, however, the BE-SONOS device seems to have more interface traps between the tunneling oxide and silicon substrate, which should be considered in designing the process conditions. Finally, 3D structures, such as the pillar type BE-SONOS device, are more suitable for next generation memory devices than other modulated tunneling oxide devices.

Successful cross-presentation of allogeneic myeloma cells by autologous alpha-type 1-polarized dendritic cells as an effective tumor antigen in myeloma patients with matched monoclonal immunoglobulins.

Yang, Deok-Hwan,Kim, Mi-Hyun,Lee, Youn-Kyung,Hong, Cheol Yi,Lee, Hyun Ju,Nguyen-Pham, Thanh-Nhan,Bae, Soo Young,Ahn, Jae-Sook,Kim, Yeo-Kyeoung,Chung, Ik-Joo,Kim, Hyeoung-Joon,Kalinski, Pawel,Lee, Je-J Springer International 2011 Annals of hematology Vol.90 No.12

<P>For wide application of a dendritic cell (DC) vaccination in myeloma patients, easily available tumor antigens should be developed. We investigated the feasibility of cellular immunotherapy using autologous alpha-type 1-polarized dendritic cells (관DC1s) loaded with apoptotic allogeneic myeloma cells, which could generate myeloma-specific cytotoxic T lymphocytes (CTLs) against autologous myeloma cells in myeloma patients. Monocyte-derived DCs were matured by adding the 관DC1-polarizing cocktail (TNF관/IL-1관/IFN-관/IFN-관/poly-I:C) and loaded with apoptotic allogeneic CD138(+) myeloma cells from other patients with matched monoclonal immunoglobulins as a tumor antigen. There were no differences in the phenotypic expression between 관DC1s loaded with apoptotic autologous and allogeneic myeloma cells. Autologous 관DC1s effectively took up apoptotic allogeneic myeloma cells from other patients with matched subtype. Myeloma-specific CTLs against autologous target cells were successfully induced by 관DC1s loaded with allogeneic tumor antigen. The cross-presentation of apoptotic allogeneic myeloma cells to 관DC1s could generate CTL responses between myeloma patients with individual matched monoclonal immunoglobulins. There was no difference in CTL responses between 관DC1s loaded with autologous tumor antigen and allogeneic tumor antigen against targeting patient's myeloma cells. Our data indicate that autologous DCs loaded with allogeneic myeloma cells with matched immunoglobulin can generate potent myeloma-specific CTL responses against autologous myeloma cells and can be a highly feasible and effective method for cellular immunotherapy in myeloma patients.</P>

<i>Momordica charantia</i> Inhibits Inflammatory Responses in Murine Macrophages via Suppression of TAK1

Yang, Woo Seok,Yang, Eunju,Kim, Min-Jeong,Jeong, Deok,Yoon, Deok Hyo,Sung, Gi-Ho,Lee, Seungihm,Yoo, Byong Chul,Yeo, Seung-Gu,Cho, Jae Youl World Scientific Publishing Company 2018 The American journal of Chinese medicine Vol.46 No.2

<P><I>Momordica charantia</I> known as bitter melon is a representative medicinal plant reported to exhibit numerous pharmacological activities such as antibacterial, antidiabetic, anti-inflammatory, anti-oxidant, antitumor, and hypoglycemic actions. Although this plant has high ethnopharmacological value for treating inflammatory diseases, the molecular mechanisms by which it inhibits the inflammatory response are not fully understood. In this study, we aim to identify the anti-inflammatory mechanism of this plant. To this end, we studied the effects of its methanol extract (Mc-ME) on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Specifically, we evaluated nitric oxide (NO) production, mRNA expression of inflammatory genes, luciferase reporter gene activity, and putative molecular targets. Mc-ME blocked NO production in a dose-dependent manner in RAW264.7 cells; importantly, no cytotoxicity was observed. Moreover, the mRNA expression levels of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 were decreased by Mc-ME treatment in a dose-dependent manner. Luciferase assays and nuclear lysate immunoblotting analyses strongly indicated that Mc-ME decreases the levels of p65 [a nuclear factor (NF)-<TEX>$ \kappa $</TEX>B subunit] and c-Fos [an activator protein (AP)-1 subunit]. Whole lysate immunoblotting assays, luciferase assays, and overexpression experiments suggested that transforming growth factor <TEX>$ \beta $</TEX>-activated kinase 1 (TAK1) is targeted by Mc-ME, thereby suppressing NF-<TEX>$ \kappa $</TEX>B and AP-1 activity via downregulation of extracellular signal-regulated kinases (ERKs) and AKT. These results strongly suggest that Mc-ME exerts its anti-inflammatory activity by reducing the action of TAK1, which also affects the activation of NF-<TEX>$ \kappa $</TEX>B and AP-1.</P>