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Xicheng Wang,Mizhen Zhao,Weimin Wu,Nicholas Kibet Korir,Yaming Qian,Zhuangwei Wang 한국유전학회 2017 Genes & Genomics Vol.39 No.5
Gibberellin (GA) is widely used to enlarge berries of seedless table grape and raisin varieties. The molecular mechanism underlying the berry-sizing effect of gibberelins is however poorly understood. We used a high-throughput deep sequencing technology to investigate the transcriptome and comparative expression profiles of berries from Vitis vinifera L. ‘Summer Black’ treated with GA3 with the aim of increasing the understanding of molecular mechanisms underlying the species’ expansion growth responses to exogenous GA3 hormone application. A total of 591 differentially expressed genes (DEGs) were detected including genes involved in fruit expansion and growth. There were four expansion genes, three cellulose synthase A catalytic subunit genes, four cellulose synthaselike protein genes and three xyloglucan endotransglucosylase genes. Differential expression of these genes could potentially explain the difference in the growth and sizes of fruits from control (CK) and GA3 treated (+GA) vines. In addition, the expression patterns of 14 DEGs were validated by qRT-PCR, and the outcomes agreed highly with the RNA-Seq results. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed to better understand the functions of these DEGs. We also identified a large number of single nucleotide polymorphism and insertion/deletion markers, which will be a rich resource for future marker development and breeding research in grape. The transcriptome analysis provides valuable information for furthering our understanding of the molecular mechanisms that regulate the fruit expansion growth, and adds to the growing foundation for future genetic and functional genomic studies in grape fruit.
Adhesion GPR123 is an indicator for recurrence and prognosis in bladder cancer
Liu Yaming,Wang Guodong,Cui Tongwei,Lv Lin 한국유전학회 2021 Genes & Genomics Vol.43 No.11
Background Bladder cancer is a common urinary cancer, and most patients sufer tumor recurrence after surgery. Identifying more prognostic biomarkers is an essential task for precious treatment. Objective To evaluate the expression and clinical signifcance of GPR123, Angiotensin-I a type of adhesion G proteincoupled receptors (aGPCRs), in bladder cancer. Methods The expressions of GPR123 in two retrospective cohorts comprised of 150 and 56 patients with bladder cancer respectively, were detected with and immunohistochemistry (IHC). Moreover, GPR123 mRNAs in 11 non-muscle-invasive bladder cancers (NMIBCs) and 11 muscle-invasive bladder cancers (MIBCs) were detected with qRT-PCR. The correlation between GPR123 and the clinicopathological characters was estimated by Chi-square test. The signifcance of GPR123 and other clinicopathological characters in recurrence and prognosis of bladder cancer was evaluated by univariate and multivariate analyses. Results GPR123 was mainly expressed in the cell membrane of bladder cancer. The percentages of high GPR123 expression in NMIBC and MIBC were 38.32 and 55.81% in cohort 1, and 16.00 and 43.90% in cohort 2. With qRT-PCR and IHC, we showed that GPR123 expression in MIBC was signifcantly higher than that in NMIBC. GPR123 was signifcantly associated with T and M stage of bladder cancer. GPR123 expression was all correlated with recurrence (disease-free survival rate), and prognosis (overall survival rate). High GPR123 expression was identifed as independent biomarker indicating easier recurrence and poorer prognosis. Conclusions GPR123 was an independent biomarker of bladder cancer for recurrence and prognosis, indicating that GPR123 detection with IHC after operation could help fnd the high-risk patients and direct the post-operational surveillance.
Zhaoyun Xu,Yaming Zhang,Bo Wang,Jian-feng Yang,Kaikai Liu 한양대학교 세라믹연구소 2016 Journal of Ceramic Processing Research Vol.17 No.8
Porous Si3N4 ceramics with varied porosities were prepared by liquid phase sintering of fibrous α-Si3N4 powder with differentmorphology after ball milling. The influence of the ball milling time on porosity, shrinkage, microstructure and mechanicalproperties of Si3N4 ceramics were investigated. With increasing of the ball milling time from 0 h to 24 hrs, the mean length andaspect ratio of the fibrous α-Si3N4 particles decreased dramatically, resulting in the porosity of porous Si3N4 ceramics decreasedfrom 55.6% to 19.3%. With the decreased length of the fibrous α-Si3N4 particles, microstructures with decreased aspect ratioof β-Si3N4 grains were formed, as a result of increased nuclei number and limited grain growth of the β-Si3N4 grains. Theflexural strength varied from 62 MPa to 205 MPa could be obtained attributed to the synergy effect of porosity andmicrostructure.
( Zhiyu Zhou ),( Junjie Wang ),( Yaming Wang ),( Zefei Zhu ),( Jiayou Du ),( Xiangqi Liu ),( Jiaxin Quan ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.11
Object detection and tracking is the basic capability of mobile robots to achieve natural human-robot interaction. In this paper, an object tracking system of mobile robot is designed and validated using improved multiple instance learning algorithm. The improved multiple instance learning algorithm which prevents model drift significantly. Secondly, in order to improve the capability of classifiers, an active sample selection strategy is proposed by optimizing a bag Fisher information function instead of the bag likelihood function, which dynamically chooses most discriminative samples for classifier training. Furthermore, we integrate the co-training criterion into algorithm to update the appearance model accurately and avoid error accumulation. Finally, we evaluate our system on challenging sequences and an indoor environment in a laboratory. And the experiment results demonstrate that the proposed methods can stably and robustly track moving object.