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( Qiang Zhang ),( Yujuan Li ),( Fangxue Xu ),( Mengmeng Zheng ),( Xiaozhi Xi ),( Xuelan Zhang ),( Chunchao Han ) 한국균학회 2017 Mycobiology Vol.45 No.2
Different endophytes isolated from the seeds of Sophora flavescens were tested for their ability to produce matrine production. Response surface methodology (RSM) was applied to optimize the medium components for the endophytic fungus. Results indicated that endophyte Aspergillus terreus had the ability to produce matrine. The single factor tests demonstrated that potato starch was the best carbon source and the combination of peptone and NH<sub>4</sub>NO<sub>3</sub> was the optimal nitrogen source for A. terreus. The model of RSM predicted to gain the maximal matrine production at 20.67 μg/L, when the potato starch was 160.68 g/L, peptone was 24.96 g/L and NH<sub>4</sub>NO<sub>3</sub> was 2.11 g/L. When cultured in the optimal medium, the matrine yield was an average of 20.63 ± 0.11 μg/L, which was consistent with the model prediction. This study offered an alternative source for the matrine production by endophytic fungus fermentation and may have far-reaching prospect and value.