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Isolation and characterization of acid-soluble bluefin tuna (Thunnus orientalis) skin collagen
Tanaka, Teruyoshi,Takahashi, Kenji,Tsubaki, Kazufumi,Hirata, Maika,Yamamoto, Keiko,Biswas, Amal,Moriyama, Tatsuya,Kawamura, Yukio The Korean Society of Fisheries and Aquatic Scienc 2018 Fisheries and Aquatic Sciences Vol.21 No.4
In this study, we isolated and characterized the acid-soluble skin collagen of Pacific bluefin tuna (PBT, Thunnus orientalis). The PBT skin collagen was composed of two ${\alpha}$ chains (${\alpha}1$ and ${\alpha}2$) and one ${\beta}$ chain. The denaturation temperature of PBT collagen was low although it was rich in proline and hydroxyproline. The primary structure of PBT skin collagen was almost identical to that of calf and salmon skin collagen; however, it differed with respect to the epitope recognition of the antibody against salmon type I collagen. These results suggest that the primary structure of skin collagen was highly conserved among animal species, although partial sequences that included the epitope structure differed among collagens.
Role of Osteoclasts in Abdominal Aortic Aneurysm and the Prevention by Food Components
Teruyoshi Tanaka 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Arterial calcification is common and contributes to the pathogenesis of occlusive vascular disease. Similar to the dynamics of bone, it is a tightly controlled process that maintains a balance between osteogenesis and osteolysis. However, whether calcium homeostasis plays a role in the development of abdominal aortic aneurysm (AAA) has not been explored. Because the detailed pathogenic mechanism of AAA is unknown, no effective therapeutic agent has been developed. We hypothesized that macrophages differentiate into osteoclasts in aneurysmal arteries and that protease byproducts contribute to aneurysm pathophysiology. We performed histological and immunohistochemical analyses and showed that macrophages positive for several osteoclast markers, including tartrate acid phosphatase, occur in great numbers in the human aneurysmal aorta, but very few occur in the human stenotic aorta and none in the nondiseased human aorta. Moreover, in situ zymography showed elevated protease activity in these cells compared with undifferentiated macrophages. Tumor necrosis factor-α and calcium phosphate stimulated this osteoclastogenic differentiation process through nuclear factor-κB, mitogen-activated protein kinases, and intracellular calcium signaling but not the receptor activator of the nuclear factor-κB ligand. Inhibition of osteoclastogenic differentiation by bisphosphonate inhibits aneurysm development in a mouse model. Furthermore, administration of puerarin, a kudzu isoflavone, significantly suppressed osteoclast differentiation and aneurysm formation in aneurysm model mice. These results suggest that differentiation of macrophages into osteoclasts contributes to the pathophysiology of aneurysmal disease. It is expected that clinical research for the treatment of abdominal aortic aneurysm targeting osteoclasts and the preventive methods using food and nutritional components will be established in the future.
Isolation and characterization of acid-soluble bluefin tuna (Thunnus orientalis) skin collagen
( Teruyoshi Tanaka ),( Kenji Takahashi ),( Kazufumi Tsubaki ),( Maika Hirata ),( Keiko Yamamoto ),( Amal Biswas ),( Tatsuya Moriyama ),( Yukio Kawamura ) 한국수산과학회(구 한국수산학회) 2018 Fisheries and Aquatic Sciences Vol.21 No.2
In this study, we isolated and characterized the acid-soluble skin collagen of Pacific bluefin tuna (PBT, Thunnus orientalis). The PBT skin collagen was composed of two α chains (α1 and α2) and one β chain. The denaturation temperature of PBT collagen was low although it was rich in proline and hydroxyproline. The primary structure of PBT skin collagen was almost identical to that of calf and salmon skin collagen; however, it differed with respect to the epitope recognition of the antibody against salmon type I collagen. These results suggest that the primary structure of skin collagen was highly conserved among animal species, although partial sequences that included the epitope structure differed among collagens.