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Current Status and Applications of Adaptive Laboratory Evolution in Industrial Microorganisms
( Surin Lee ),( Pil Kim ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.6
Adaptive laboratory evolution (ALE) is an evolutionary engineering approach in artificial conditions that improves organisms through the imitation of natural evolution. Due to the development of multi-level omics technologies in recent decades, ALE can be performed for various purposes at the laboratory level. This review delineates the basics of the experimental design of ALE based on several ALE studies of industrial microbial strains and updates current strategies combined with progressed metabolic engineering, in silico modeling and automation to maximize the evolution efficiency. Moreover, the review sheds light on the applicability of ALE as a strain development approach that complies with non-recombinant preferences in various food industries. Overall, recent progress in the utilization of ALE for strain development leading to successful industrialization is discussed.
Lee, Suseung,Choi, Inhee,Hong, Surin,In Yang, Young,Lee, Jeongjin,Kang, Taewook,Yi, Jongheop Royal Society of Chemistry 2009 Chemical communications Vol.2009 No.41
<P>A highly specific interaction between a metal-deficient metalloenzyme and metal ion has been utilized in the selective detection of the metal ion by surface plasmon resonance spectroscopy (SPRS). The use of SPRS and Cu-demetallated superoxide dismutase (E,Zn-SOD1) as a sensing actuator allows one to selectively and <I>in situ</I> detect Cu<SUP>2+</SUP> without any interference that other spectroscopic methods may have.</P> <P>Graphic Abstract</P><P>A highly specific interaction between a Cu-demetallated superoxide dismutase and Cu<SUP>2+</SUP> ion has been utilized in the selective detection of the Cu<SUP>2+</SUP> ion by surface plasmon resonance spectroscopy (SPRS). <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b910666a'> </P>
Lee, Yong-Min,Kim, Surin,Ohkubo, Kei,Kim, Kyung-Ha,Nam, Wonwoo,Fukuzumi, Shunichi American Chemical Society 2019 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.141 No.6
<P>Outer-sphere electron transfer from styrene, thioanisole, and toluene derivatives to a triflic acid (HOTf)-bound nonheme Mn(IV)-oxo complex, [(N4Py)Mn<SUP>IV</SUP>(O)]<SUP>2+</SUP>-(HOTf)<SUB>2</SUB> (N4Py = <I>N</I>,<I>N</I>-bis(2-pyridylmethyl)-<I>N</I>-bis(2-pyridyl)methylamine), has been shown to be the rate-determining step of different types of redox reactions such as epoxidation, sulfoxidation, and hydroxylation of styrene, thioanisole, and toluene derivatives, respectively, by [(N4Py)Mn<SUP>IV</SUP>(O)]<SUP>2+</SUP>-(HOTf)<SUB>2</SUB>. The rate constants of HOTf-promoted epoxidation of all styrene derivatives with [(N4Py)Mn<SUP>IV</SUP>(O)]<SUP>2+</SUP> and electron transfer from electron donors to [(N4Py)Mn<SUP>V</SUP>(O)]<SUP>2+</SUP> exhibit a remarkably unified correlation with the driving force of outer-sphere electron transfer in light of the Marcus theory of electron transfer. The same electron-transfer driving force dependence is observed in the oxygen atom transfer from [(N4Py)Mn<SUP>IV</SUP>(O)]<SUP>2+</SUP>-(HOTf)<SUB>2</SUB> to thioanisole derivatives as well as in the hydrogen atom transfer from toluene derivatives to [(N4Py)Mn<SUP>IV</SUP>(O)]<SUP>2+</SUP>-(HOTf)<SUB>2</SUB>. Thus, mechanisms of oxygen atom transfer (epoxidation and sulfoxidation) reactions of styrene and thioanisole derivatives and hydrogen atom transfer (hydroxylation) reactions of toluene derivatives by [(N4Py)Mn<SUP>IV</SUP>(O)]<SUP>2+</SUP>-(HOTf)<SUB>2</SUB> have been unified for the first time as the same reaction pathway via outer-sphere electron transfer, followed by the fast bond-forming step, which exhibits the singly unified electron-transfer driving force dependence of the rate constants as outer-sphere electron-transfer reactions. In the case of the epoxidation of <I>cis</I>-stilbene by [(N4Py)Mn<SUP>IV</SUP>(O)]<SUP>2+</SUP>-(HOTf)<SUB>2</SUB>, the isomerization of <I>cis</I>-stilbene radical cation to <I>trans</I>-stilbene radical cation occurs after outer-sphere electron transfer from <I>cis</I>-stilbene to [(N4Py)Mn<SUP>IV</SUP>(O)]<SUP>2+</SUP>-(HOTf)<SUB>2</SUB> to yield <I>trans</I>-stilbene oxide selectively, which is also taken as evidence for the occurrence of electron transfer in the acid-catalyzed epoxidation.</P> [FIG OMISSION]</BR>
Hong, Surin,Lee, Suseung,Yi, Jongheop Springer 2011 Nanoscale research letters Vol.6 No.1
<P>A highly sensitive and molecular size-selective method for the detection of proteins using heteroliganded gold nanoislands and localized surface plasmon resonance (LSPR) is described. Two different heteroligands with different chain lengths (3-mercaptopionicacid and decanethiol) were used in fabricating nanoholes for the size-dependent separation of a protein in comparison with its aggregate. Their ratios on gold nanoisland were optimized for the sensitive detection of superoxide dismutase (SOD1). This protein has been implicated in the pathology of amyotrophic lateral sclerosis (ALS). Upon exposure of the optimized gold nanoisland to a solution of SOD1 and aggregates thereof, changes in the LSPR spectra were observed which are attributed to the size-selective and covalent chemical binding of SOD1 to the nanoholes. With a lower detection limit of 1.0 ng/ml, the method can be used to selectively detect SOD1 in the presence of aggregates at the molecular level.</P>
서수린(Surin Seo),형원석(Won Seok William Hyung),이승훈(Seung-Hoon Lee),김준형(Junhyung Kim),최광연(Kwang-Yeon Choi),윤현철(HyunChul Youn),정현강(Hyun-Ghang Jeong) 대한노인정신의학회 2023 노인정신의학 Vol.27 No.2
Objective: Cognitive dysfunction is frequently reported after surgery and anesthesia in elderly patients. This study aims to investigate the incidence of dementia for eight years after anesthesia. Methods: We extracted the data between 2007 and 2020 from the Health Insurance Review and Assessment Service Database. We analyzed risk of developing dementia after anesthesia using Cox proportional hazard models. Results: Data were collected from 62,541 patient. Among them, subjects with and without anesthesia were 15,857 and 46,684, respectively. Subjects with anesthesia had more hypertension, diabetes mellitus and hyperlipemia than those without anesthesia. The risk of dementia incidence was found to increase 1.7 times in subjects with anesthesia (hazard ratio=1.751, 95% confidence interval=1.596-1.921). Even after the correction for gender, age, and comorbidity, the statistical significance of this risk remained (hazard ratio=1.662, 95% confidence interval=1.512-1.827). Conclusion: Our findings suggest that anesthesia possibly contribute on cognitive impairment. Clinician might need closed observation on cognitive function in patients after surgery and anesthesia.
원심모형실험을 이용한 모래지반에 관입된 계류선 거동 평가
이훈용(Hoon Yong Lee),김수린(Surin Kim),김재현(Jaehyun Kim),김동수(Dong-Soo Kim),추연욱(Yun Wook Choo),권오순(Osoon Kwon) 한국해양공학회 2014 韓國海洋工學會誌 Vol.28 No.1
When an anchor penetrates and is installed under a seabed, a portion of the mooring line connected to the anchor is also embedded under the seabed. This embedded mooring line affects the capacity of the anchor in two ways. First, the frictional resistance that occurs between the mooring line and the seabed reduces the pulling force acting on the anchor. Second, the embedded part of the mooring line forms a reverse catenary shape due to the bearing resistance of the soil, so that an inclined pulling force is applied to the anchor. To evaluate the mooring line’s effect on the capacity of an anchor in sand, centrifuge model tests performed using two relative sand densities of 76% and 51% while changing the anchor depths. The test results showed that the load is reduced much more in deep and dense sand, and the inclination angle of the load is lower in shallow and loose sand.
Yang, Young In,Choi, Inhee,Hong, Surin,Lee, Suseung,Kang, Taewook,Lee, Hyunjoo,Yil, Jongheop American Scientific Publishers 2010 Journal of Nanoscience and Nanotechnology Vol.10 No.5
<P>A simple, accurate method for detecting metal ions in an aqueous solution using functionalized gold nanorods (AuNRs) is described. The method involves the complexing of divalent metal ions with poly(acrylic acid) (PAA) and, the localized surface plasmon resonance (LSPR) phenomena of AuNRs. Changes in the longitudinal surface plasmon bands (LSPBs) were monitored using aggregates of PAA-coated AuNRs with various divalent metal ions via UV-vis spectroscope. Functionalized AuNRs underwent robust aggregate formation by chelation with divalent metal ions (e.g., Cu2+, Zn2+, Cd2+, and Fe2+). Copper ions formed largest aggregates within 2 h, because complexation between Cu2+ and dicarboxylate has the highest deltaH and -deltaG values. This process represents an easy and useful method for detecting certain divalent metal ions, and the aggregates are also, in some cases, clearly visible to the naked eye.</P>