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RISS 인기검색어
- 검색키워드
- 저자 : ( Steve S. Sommer ) (검색결과 2 건)
- 무료
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- 유료
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Single-base Discrimination Mediated by Proofreading Inert Allele Specific Primers
Lin-Ling, Chen,Zhang, Jia,Sommer, Steve S.,Li, Kai Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.1
The role of 3' exonuclease excision in DNA polymerization was evaluated for primer extension using inert allele specific primers with exonuclease-digestible ddNMP at their 3' termini. Efficient primer extension was observed in amplicons where the inert allele specific primers and their corresponding templates were mismatched. However, no primer-extended products were yielded by matched amplicons with inert primers. As a control, polymerase without proofreading activity failed to yield primer extended products from inert primers regardless of whether the primers and templates were matched or mismatched. These data indicated that activation was undertaken for the inert allele specific primers through mismatch proofreading. Complementary to our previously developed SNP-operated on/off switch, in which DNA polymerization only occurs in matched amplicon, this new mutation detection assay mediated by $exo^+$ DNA polymerases has immediate applications in SNP analysis independently or in combination of the two assays.
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Single-base Discrimination Mediated by Proofreading Inert Allele Specific Primers
( Lin Ling Chen ),( Zhang Jia ),( Steve S. Sommer ),( Li Kai ) 생화학분자생물학회 2005 BMB Reports Vol.38 No.1
The role of 3` exonuclease excision in DNA polymerization was evaluated for primer extension using inert allele specific primers with exonuclease-digestible ddNMP at their 3` termini. Efficient primer extension was observed in amplicons where the inert allele specific primers and their corresponding templates were mismatched. However, no primer-extended products were yielded by matched amplicons with inert primers. As a control, polymerase without proofreading activity failed to yield primer-extended products from inert primers regardless of whether the primers and templates were matched or mismatched. These data indicated that activation was undertaken for the inert allele specific primers through mismatch proofreading. Complementary to our previously developed SNP-operated on/off switch, in which DNA polymerization only occurs in matched amplicon, this new mutation detection assay mediated by exo+ DNA polymerases has immediate applications in SNP analysis independently or in combination of the two assays.
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