Study on the Industrial Process of Rubber Anti-oxidant RD

Liu, Yu,Gao, Qinyu,Liu, Lianxin,Shi, Guangxia Korean Chemical Society 2011 대한화학회지 Vol.55 No.5

This paper is on the industrial synthesis process of anti-oxidant RD ((2,2,4-trimethyl-1,2-dihydroquinoline polymer $(C_{12}H_{15}N)_n$. n=2-4)).The content of dimer, trimer and tetramer of RD as the inspection targets, using the orthogonal design method - take the ratios of keto-amine, the reaction time, the reaction temperatures and the ratios of catalyst acid-amine as inspect factors - to optimized the reaction condition. The results indicate that the best ratio of keto-amine is 2:1, the time of salification and condensation is 3 hours and 7.5 hours. The range of temperature of salification and condensation is $135^{\circ}C$ and $120-125^{\circ}C$, and that the best ratio of acid-amine is 0.2: 1 (the proportion is the concentration ratio for mole). Under the optimization conditions, the yield of RD was stabilized and content of RD more than 45%.

Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice

Yu-Ying Liu,Wentao Yang,Shaohua Shi,Ya-Jie Li,Liang Zhao,Chunwei Shi,Fangyu Zhou,Yanlong Jiang,Jingtao Hu,Wei Gu,Gui-Lian Yang,Chun-feng Wang 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.2

Goose parvovirus (GPV) continues to be a threat to goose farms and has significant economic effects on the production of geese. Current commercially available vaccines only rarely prevent GPV infection. In our study, Lactobacillus (L.) plantarum NC8 was selected as a vector to express the VP2 gene of GPV, and recombinant L. plantarum pSIP409-VP2/NC8 was successfully constructed. The molecular weight of the expressed recombinant protein was approximately 70 kDa. Mice were immunized with a 2 × 109 colony-forming unit/200 mL dose of the recombinant L. plantarum strain, and the ratios and numbers of CD11c+, CD3+CD4+, CD3+CD8+, and interferon gamma- and tumor necrosis factor alpha-expressing spleen lymphocytes in the pSIP409-VP2/NC8 group were higher than those in the control groups. In addition, we assessed the capacity of L. plantarum SIP409-VP2/NC8 to induce secretory IgA production. We conclude that administered pSIP409-VP2/NC8 leads to relatively extensive cellular responses. This study provides information on GPV infection and offers a clear framework of options available for GPV control strategies.

Virtual Non-Contrast Computer Tomography (CT) with Spectral CT as an Alternative to Conventional Unenhanced CT in the Assessment of Gastric Cancer

Tian, Shi-Feng,Liu, Ai-Lian,Wang, He-Qing,Liu, Jing-Hong,Sun, Mei-Yu,Liu, Yi-Jun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6

Objective: The purpose of this study was to evaluate computed tomography (CT) virtual non-contrast (VNC) spectral imaging for gastric carcinoma. Materials and Methods: Fifty-two patients with histologically proven gastric carcinomas underwent gemstone spectral imaging (GSI) including non-contrast and contrast-enhanced hepatic arterial, portal venous, and equilibrium phase acquisitions prior to surgery. VNC arterial phase (VNCa), VNC venous phase (VNCv), and VNC equilibrium phase (VNCe) images were obtained by subtracting iodine from iodine/water images. Images were analyzed with respect to image quality, gastric carcinoma-intragastric water contrast-to-noise ratio (CNR), gastric carcinoma-perigastric fat CNR, serosal invasion, and enlarged lymph nodes around the lesions. Results: Carcinoma-water CNR values were significantly higher in VNCa, VNCv, and VNCe images than in normal CT images (2.72, 2.60, 2.61, respectively, vs 2.35, $p{\leq}0.008$). Carcinoma-perigastric fat CNR values were significantly lower in VNCa, VNCv, and VNCe images than in normal CT images (7.63, 7.49, 7.32, respectively, vs 8.48, p< 0.001). There were no significant differences of carcinoma-water CNR and carcinoma-perigastric fat CNR among VNCa, VNCv, and VNCe images. There was no difference in the determination of invasion or enlarged lymph nodes between normal CT and VNCa images. Conclusions: VNC arterial phase images may be a surrogate for conventional non-contrast CT images in gastric carcinoma evaluation.

Serum Insulin-Like Growth Factor Binding Protein 7 as a Potential Biomarker in the Diagnosis and Prognosis of Esophagogastric Junction Adenocarcinoma

Can-Tong Liu,Yi-Wei Xu,Hong Guo,Chao-Qun Hong,Xin-Yi Huang,Yu-Hao Luo,Shi-Han Yang,Ling-Yu Chu,En- Min Li,Yu-Hui Peng 거트앤리버 소화기연관학회협의회 2020 Gut and Liver Vol.14 No.6

Background/Aims: Esophagogastric junction adenocarcinoma (EJA) is a malignant tumor associated with high morbidity and has attracted increasing attention due to a rising incidence and low survival rate. Pathological biopsy is the gold standard for diagnosis, but noninvasive and effective tests are lacking, resulting in diagnoses at advanced stages. This study explored the diagnostic value of insulin-like growth factor binding protein 7 (IGFBP7) in EJA. Methods: A total of 120 EJA patients and 88 normal controls were recruited, and their serum levels of IGFBP7 were measured by enzymelinked immunosorbent assay. Receiver operating characteristic (ROC) curve analysis was used to assess the diagnostic value, and Pearson chi-square analysis was used to evaluate the correlation between IGFBP7 and clinical parameters. Kaplan- Meier survival analysis was carried out to assess the effect of IGFBP7 on overall survival (OS). Results: The levels of IGFBP7 were higher in both early- and late-stage EJA patients than in normal controls (p<0.001). The area under the ROC curve for EJA patients was 0.794 (95% confidence interval [CI], 0.733 to 0.854), with a cutoff value of 2.716 ng/mL, a sensitivity of 63.3% (95% CI, 54.0% to 71.8%) and a specificity of 90.9% (95% CI, 82.4% to 95.7%). For the diagnosis of early-stage EJA, the same cutoff value and specificity were obtained, but the sensitivity of IGFBP7 was 54.3% (95% CI, 36.9% to 70.8%). Patients with low IGFBP7 protein expression had lower OS than those with high expression (p=0.034). The multivariate analysis showed that IGFBP7 is an independent prognostic factor for EJA (p=0.011). Conclusions: Serum IGFBP7 acts as a potential diagnostic and prognostic marker for EJA.

Separating machining errors of S-shaped samples based on the comprehensive error field of five-axis machine tools

Shi Wu,Yupeng Wang,Xian-li Liu,Zhengdong Fan,Tai Yu 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.1

In the present study, a new method is proposed to separate machining errors of a 5-axis machine tool with a double turntable. Moreover, a high-precision compensation model is established to calculate machining errors of thin-walled parts in a 5-axis machine tool. In this regard, a comprehensive error model of the S-shaped sample part considering geometric, thermal, and machining errors is established. Then based on the homogeneous transformation matrix, the geometric error models of translational axes and the A and C axes of the rotation axis are established. Accordingly, geometric errors of each motion axes are identified and measured. Meanwhile, the thermal error of the spindle is measured, and the thermal error model of the machine tool along X/Y/Z directions is established using the multiple linear regression method. Finally, the S-shaped sample is processed and the on-machine measurement of the sample surface is carried out. Based on the obtained machining error from the separation method, the distribution of the total surface error at different curvatures of the S-shaped sample is analyzed, and the distributions of geometric and thermal errors of the machine tool are obtained. It is found that when the geometric and thermal errors of the machine tool are compensated, the measured machining error of each point of the S-shaped sample can be reduced by 10-15 μm compared with that before compensation.

Kinetics and computational docking studies on the inhibition of tyrosinase induced by oxymatrine.

Liu, Xiao-Xia,Sun, Shi-Qing,Wang, Yu-Jie,Xu, Wei,Wang, Yi-Fang,Park, Daeui,Zhou, Hai-Meng,Han, Hong-Yan Humana Press 2013 Applied biochemistry and biotechnology Vol.169 No.1

<P>A combination of enzymatic inhibition kinetics and computational prediction was employed to search for an effective inhibitor of tyrosinase. We found that oxymatrine significantly inhibited tyrosinase, and that this reaction was not accompanied by detectable conformational changes. Kinetic analysis showed that oxymatrine reversibly inhibited tyrosinase in a mixed-type manner. Measurements of intrinsic and ANS-binding fluorescences showed that oxymatrine did not induce any conspicuous changes in the tertiary structure. We also conducted a docking simulation between tyrosinase and oxymatrine using two docking programs, Dock6.3 and AutoDock4.2 (binding energy was -118.81 kcal/mol for Dock6 and -8.04 kcal/mol for AutoDock4). The results also suggested that oxymatrine interacts mostly with the residues of CYS83 and HIS263 in the active site of tyrosinase. This strategy of predicting tyrosinase inhibition by simulation of docking coupling with kinetics may prove useful in screening for potential tyrosinase inhibitors. Knowledge of tyrosinase inhibition can provide medical, cosmetic, and agricultural applications. Our study suggests that oxymatrine is an important agent for various applications related to pigment formation.</P>

Mouse Nerve Growth Factor Facilitates the Growth of Interspinal Schwannoma Cells by Activating NGF Receptors

Liu, Shu Yi,Liu, Sheng Ze,Li, Yu,Chen, Shi The Korean Neurosurgical Society 2019 Journal of Korean neurosurgical society Vol.62 No.6

Objective : Nerve growth factor (NGF) is a member of the neurotrophic factor family and plays a vital role in the physiological processes of organisms, especially in the nervous system. Many recent studies have reported that NGF is also involved in the regulation of tumourigenesis by either promoting or suppressing tumor growth, which depends on the location and type of tumor. However, little is known regarding the effect of NGF on interspinal schwannoma (IS). In the present study, we aimed to explored whether mouse nerve growth factor (mNGF), which is widely used in the clinic, can influence the growth of interspinal schwannoma cells (ISCs) isolated from IS in vitro. Methods : ISCs were isolated, cultured and identified by S-100 with immunofluorescence analysis. S-100-positive cells were divided into five groups, and separately cultured with various concentrations of mNGF (0 [phosphate buffered saline, PBS], 40, 80, 160, and 320 ng/mL) for 24 hours. Western blot and quantantive real time polymerase chain reaction (PCR) were applied to detect tyrosine kinase A (TrkA) receptor and p75 neurotrophin receptor ($p75^{NTR}$) in each group. Crystal violet staining was selected to assess the effect of mNGF (160 ng/mL) on ISCs growth. Results : ISCs growth was enhanced by mNGF in a dose-dependent manner. The result of crystal violet staining revealed that it was significantly strengthened the cells growth kinetics when cultured with 160 ng/mL mNGF compared to PBS group. Western blot and quantantive real time PCR discovered that TrkA receptor and mRNA expression were both up-regualated under the condition of mNGF, expecially in 160 ng/mL, while the exoression of $p75^{NTR}$ demonstrated no difference among groups. Conclusion : From these data, we conclude that exogenous mNGF can facilitate ISC growth by activating both TrkA receptor and $p75^{NTR}$. In addition, patients who are suffering from IS should not be administered mNGF in the clinic.

Prediction of Lung Cancer Based on Serum Biomarkers by Gene Expression Programming Methods

Yu, Zhuang,Chen, Xiao-Zheng,Cui, Lian-Hua,Si, Hong-Zong,Lu, Hai-Jiao,Liu, Shi-Hai Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

In diagnosis of lung cancer, rapid distinction between small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) tumors is very important. Serum markers, including lactate dehydrogenase (LDH), C-reactive protein (CRP), carcino-embryonic antigen (CEA), neurone specific enolase (NSE) and Cyfra21-1, are reported to reflect lung cancer characteristics. In this study classification of lung tumors was made based on biomarkers (measured in 120 NSCLC and 60 SCLC patients) by setting up optimal biomarker joint models with a powerful computerized tool - gene expression programming (GEP). GEP is a learning algorithm that combines the advantages of genetic programming (GP) and genetic algorithms (GA). It specifically focuses on relationships between variables in sets of data and then builds models to explain these relationships, and has been successfully used in formula finding and function mining. As a basis for defining a GEP environment for SCLC and NSCLC prediction, three explicit predictive models were constructed. CEA and NSE are requentlyused lung cancer markers in clinical trials, CRP, LDH and Cyfra21-1 have significant meaning in lung cancer, basis on CEA and NSE we set up three GEP models-GEP 1(CEA, NSE, Cyfra21-1), GEP2 (CEA, NSE, LDH), GEP3 (CEA, NSE, CRP). The best classification result of GEP gained when CEA, NSE and Cyfra21-1 were combined: 128 of 135 subjects in the training set and 40 of 45 subjects in the test set were classified correctly, the accuracy rate is 94.8% in training set; on collection of samples for testing, the accuracy rate is 88.9%. With GEP2, the accuracy was significantly decreased by 1.5% and 6.6% in training set and test set, in GEP3 was 0.82% and 4.45% respectively. Serum Cyfra21-1 is a useful and sensitive serum biomarker in discriminating between NSCLC and SCLC. GEP modeling is a promising and excellent tool in diagnosis of lung cancer.

Property Alignment of Linked Data Based on Similarity between Functions

Yu Liu,Shi-Hong Chen,Jin-Guang Gu 보안공학연구지원센터 2015 International Journal of Database Theory and Appli Vol.8 No.4

Owing to the complex structure and multi-meaning, property alignment is generally regarded as a challenging problem in the context of linked data. In this paper, we propose a novel method to align properties between datasets of linked data. Considering the role of properties in RDF triples, we regard all properties of linked data as property functions, and convert the problem of property alignment to the similarity evaluation between property functions, while the equivalent instances as inputs of property functions. Based on the similarity of property functions, the property alignment process of linked data is introduced. In order to prove the validity, we use the method to align properties in five representative domains between DBpeida and YAGO, DBpedia and LinkedGeoData respectively. The experimental results show that our method is independent of the property naming rules and can retrieve some matching properties ignored by other methods. In addition, our method requires fewer entity co-reference links than the link statistical approach.

Transjugular intrahepatic portosystemic shunt for pyrrolidine-alkaloid-induced hepatic sinusoidal obstruction syndrome in a patient with situs inversus totalis and portal vein thrombus

Yu-Guan Xie,Chen Xu,Hai-Bin Shi,Sheng Liu,Wei Yang,Wei Tian,Wei-Zhong Zhou 소화기인터벤션의학회 2023 International journal of gastrointestinal interven Vol.12 No.1

We here report a rare case of pyrrolidine-alkaloid-induced hepatic sinusoidal obstruction syndrome (PA-HSOS) complicated with situs inversus totalis and portal vein thrombus (PVT). The patient had abdominal distention and ascites, which were not responsive to conservative medical treatment or peritoneal drainage. Transjugular intrahepatic portosystemic shunt (TIPS) was successfully performed in the patient via the left internal jugular vein approach. After the procedure, abdominal distension and ascites gradually resolved. The TIPS procedure could be considered as a useful treatment for PA-HSOS patients with PVT.