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Choi, Eun H.,Kim, Dong M.,Choi, Sung‐,Wook,Eremin, Sergei A.,Chun, Hyang S. Blackwell Publishing Ltd 2011 International journal of food science & technology Vol.46 No.10
<P><B>Summary</B></P><P>The purpose of this study was to develop a highly sensitive fluorescence polarisation immunoassay (FPIA) for the detection of zearalenone (ZEN). The method was optimised and validated to examine the feasibility of performing FPIA using several tracers synthesised from different fluorescence labels and chemicals structurally mimicking ZEN. Optimum extraction conditions were determined, and 4‐(aminomethyl) fluorescein‐labelled ZEN tracer (ZEN‐4AMF) was selected as the tracer. None of the tracers mimicking ZEN gave a favourable response with the monoclonal anti‐ZEN antibody. When tested on a corn matrix, the FPIA showed a detection limit of 77 μg kg<SUP>−1</SUP> within 3 min, excluding extraction time. Recovery of ZEN averaged 101% (intraday) and 109% (interday), and trueness averaged 111%. Ruggedness was satisfactory but cross‐reactivity with ZEN analogues was relatively high. These results suggest that the current FPIA for the detection of ZEN has a potential as an easy and rapid screening tool for ZEN and its analogues in corn.</P>
( Won Bo Shim ),( Boris B. Dzantiev ),( Sergei A. Eremin ),( Duck Hwa Chung ) 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.1
Individual immunochromatographic assays (ICG) for ochratoxin A (OTA) and zearalenone (ZEA) were optimized and used in the development of a one-step simultaneous immunochromatographic assay (OS-ICG) for the rapid multianalysis of two mycotoxins in corn samples. The nitrocellulose membrane of the OS-ICG was treated with OTA-bovine serum albumin (BSA), ZEA-ovalbumin (OVA), and anti-mouse IgG in the OTA test, ZEA test, and control zones, respectively. Monoclonal antibody-gold conjugates (OTA3 MAb-gold and ZEA2C5 MAb-gold) were sprayed onto the conjugate pad. The visual detection limits were 2.5 and 5 ng/ml for OTA and ZEA, resepectively, and the results were obtained within 15 min after starting the analysis. An efficient, simple, and rapid extraction method using 30% MeOH/PBS was established and validated by analyzing the corn samples spiked with OTA/ZEA mixtures (0/0, 5/10, 10/20, and 20/30 μg/kg). The cut-off values of the OS-ICG for the spiked corn were 5 and 10 μg/kg for OTA and ZEA, respectively. Natural corn samples were analyzed by OS-ICG, direct competitive enzyme-linked immunosorbent assay (DC-ELISA), and HPLC. Results of the OS-ICG were in good agreement with those obtained by DC-ELISA and HPLC. The developed OS-ICG offers a rapid, easy-to-use, and portable analytical system and can be used as a convenient qualitative tool for the on-site simultaneous determination of OTA and ZEA in cereals, food, and agricultural products in one analytical cycle.