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Therapeutic Potential of Stem Cells Strategy for Cardiovascular Diseases
Lee, Chang Youn,Kim, Ran,Ham, Onju,Lee, Jihyun,Kim, Pilseog,Lee, Seokyeon,Oh, Sekyung,Lee, Hojin,Lee, Minyoung,Kim, Jongmin,Chang, Woochul Hindawi Publishing Corporation 2016 Stem cells international Vol.2016 No.-
<P>Despite development of medicine, cardiovascular diseases (CVDs) are still the leading cause of mortality and morbidity worldwide. Over the past 10 years, various stem cells have been utilized in therapeutic strategies for the treatment of CVDs. CVDs are characterized by a broad range of pathological reactions including inflammation, necrosis, hyperplasia, and hypertrophy. However, the causes of CVDs are still unclear. While there is a limit to the currently available target-dependent treatments, the therapeutic potential of stem cells is very attractive for the treatment of CVDs because of their paracrine effects, anti-inflammatory activity, and immunomodulatory capacity. Various studies have recently reported increased therapeutic potential of transplantation of microRNA- (miRNA-) overexpressing stem cells or small-molecule-treated cells. In addition to treatment with drugs or overexpressed miRNA in stem cells, stem cell-derived extracellular vesicles also have therapeutic potential because they can deliver the stem cell-specific RNA and protein into the host cell, thereby improving cell viability. Here, we reported the state of stem cell-based therapy for the treatment of CVDs and the potential for cell-free based therapy.</P>
( Ran Kim ),( Seokyeon Lee ),( Jihyun Lee ),( Minji Kim ),( Won Jung Kim ),( Hee Won Lee ),( Min Young Lee ),( Jongmin Kim ),( Woochul Chang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.8
Exosomes are small membranous vesicles which contain abundant RNA molecules, and are transferred from releasing cells to uptaking cells. MicroRNA (miRNA) is one of the transferred molecules affecting the adopted cells, including glioma cells. We hypothesized that mesenchymal stem cells (MSCs) can secrete exosomes loading miRNA and have important effects on the progress of gliomas. To determine these effects by treating exosomal miRNA in culture media of miRNA mimic transfected MSCs, we assessed the in vitro cell proliferation and invasion capabilities, and the expression level of relative proteins associated with cell apoptosis, growth and migration. For animal studies, the mice injected with U87 cells were exposed to exosomes derived from miRNA-584-5p transfected MSCs, to confirm the influence of exosomal miRNA on the progress of glioma. Based on our results, we propose a new targeted cancer therapy wherein exosomes derived from miRNA transfected MSCs could be used to modulate tumor progress as the anticancer vehicles. [BMB Reports 2018; 51(8): 406-411]
<i>Salvia miltiorrhiza</i> enhances the survival of mesenchymal stem cells under ischemic conditions
Kim, Ran,Lee, Seokyeon,Lee, Chang Youn,Yun, Hwayoung,Lee, Hojin,Lee, Min Young,Kim, Jongmin,Jeong, Jee‐,Yeong,Baek, Kyungmin,Chang, Woochul John Wiley and Sons Inc. 2018 Journal of pharmacy and pharmacology Vol.70 No.9
<P><B>Abstract</B></P><P><B>Objectives</B></P><P>To validate the enhanced therapeutic effect of <I>Salvia miltiorrhiza</I> Bunge (SM) for brain ischemic stroke through the anti‐apoptotic and survival ability of mesenchymal stem cells (MSCs).</P><P><B>Methods</B></P><P>The viability and the expression level of cell apoptotic and survival‐related proteins in MSCs by treatment of SM were assessed <I>in vitro</I>. In addition, the infarcted brain region and the behavioural changes after treatment of MSCs with SM were confirmed in rat middle cerebral artery occlusion (MCAo) models.</P><P><B>Key findings</B></P><P>We demonstrated that SM attenuates apoptosis and improves the cell viability of MSCs. In the rat MCAo model, the recovery of the infarcted region and positive changes of behaviour are observed after treatment of MSCs with SM.</P><P><B>Conclusions</B></P><P>The therapy using SM enhances the therapeutic effect for brain ischemic stroke by promoting the survival of MSCs. This synergetic effect thereby proposes a new experimental approach of traditional Chinese medicine and stem cell‐based therapies for patients suffering from a variety of diseases.</P>