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Magnetic Nanozyme-Linked Immunosorbent Assay for Ultrasensitive Influenza A Virus Detection
Oh, Sangjin,Kim, Jeonghyo,Tran, Van Tan,Lee, Dong Kyu,Ahmed, Syed Rahin,Hong, Jong Chul,Lee, Jaewook,Park, Enoch Y.,Lee, Jaebeom American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.15
<P>Rapid and sensitive detection of influenza virus is of soaring importance to prevent further spread of infections and adequate clinical treatment. Herein, an ultrasensitive colorimetric assay called magnetic nano(e)zyme-linked immunosorbent assay (MagLISA) is suggested, in which silica-shelled magnetic nanobeads (MagNBs) and gold nanoparticles are combined to monitor influenza A virus up to femtogram per milliliter concentration. Two essential strategies for ultrasensitive sensing are designed, i.e., facile target separation by MagNBs and signal amplification by the enzymelike activity of gold nanozymes (AuNZs). The enzymelike activity was experimentally and computationally evaluated, where the catalyticity of AuNZ was tremendously stronger than that of normal biological enzymes. In the spiked test, a straightforward linearity was presented in the range of 5.0 × 10<SUP>-15</SUP>-5.0 × 10<SUP>-6</SUP>g·mL<SUP>-1</SUP> in detecting the influenza virus A (New Caledonia/20/1999) (H1N1). The detection limit is up to 5.0 × 10<SUP>-12</SUP> g·mL<SUP>-1</SUP> only by human eyes, as well as up to 44.2 × 10<SUP>-15</SUP> g·mL<SUP>-1</SUP> by a microplate reader, which is the lowest record to monitor influenza virus using enzyme-linked immunosorbent assay-based technology as far as we know. Clinically isolated human serum samples were successfully observed at the detection limit of 2.6 PFU·mL<SUP>-1</SUP>. This novel MagLISA demonstrates, therefore, a robust sensing platform possessing the advances of fathomable sample separation, enrichment, ultrasensitive readout, and anti-interference ability may reduce the spread of influenza virus and provide immediate clinical treatment.</P> [FIG OMISSION]</BR>
Chae, Oh B.,Park, Sangjin,Ryu, Ji Heon,Oh, Seung M. The Electrochemical Society 2013 Journal of the Electrochemical Society Vol.160 No.1
<P>Two different nano-sized zinc oxide (ZnO) composite electrodes are prepared and their electrode performances are compared, in which conductive carbon is added as either a physical mixture (electrode <I>A</I>) or a matrix (electrode <I>B</I>). The ZnO composite electrode prepared according to the commonly used method (electrode <I>A</I>) swells upon lithiation but does not contract in the forthcoming de-lithiation period. Due to this irrevocable electrode expansion, the electrically conductive network comprising the ZnO and carbon particles is destroyed to give rapid capacity decay. The other electrode that is prepared as a countermeasure for the volume-change problem (electrode <I>B</I>), however, shows a reversible swelling/contraction behavior to give a stable cycle performance (reversible capacity of 700 mA h g<SUP>−1</SUP> up to 200 cycles) with the electrode integrity being retained. In the latter electrode, the carbon matrix that surrounds the embedded ZnO particles seems to accommodate the volume change of ZnO component with a strong resilient force.</P>
시맨틱 세그멘테이션 기반의 P&ID 심볼 탐지에 대한 연구
오상진(Sangjin Oh),이현(Hyun Lee),이정규(Jeongkyu Lee),이영환(Younghwan Lee),정은경(Eunkyung Jeong),이현식(Hyunsik Lee) (사)한국CDE학회 2020 한국CDE학회 논문집 Vol.25 No.4
Although the Fourth Industrial Revolution comes to reality rapidly, construction industry is going through a difficult time to adopt new technologies. Also, improving productivity is one of the most urgent issues for major construction companies. However, reading information and digitizing them from imaged drawings takes much time and it becomes a reason for low productivity. Thus, in this paper, we propose a method to recognize symbols in P&ID (piping and instrumentation diagram) using neural networks for Semantic Segmentation. First, crop a drawing into small patches and label on them 8 classes of symbol. Then, Train U-net and FCN with 2,500 patches with annotation. After training, results of recognition are displayed with color code on imaged drawings. Finally, we run tests with 5 new P&ID drawings and scored the performance of our recognition models.
Changhoon Oh,Sangjin Kim,Sangjin Lee,Eung Ryeol Seo 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.8
This study investigates the effects of various factors of a residential hood on its capability of removing pollutants generated during the cooking process. For this purpose, a number of numerical simulations are performed to quantify the effect of each parameter, including flow rate, size of the hood, filter area, the location of cooking pots, and installation height of the hood. Simulation results reveal that the depth of the hood and installation location are the most important among the parameters. Optimizing the inlet also improved the capture efficiency by a few percent without design change or a penalty in the flow rate. The capture efficiencies of the hoods differ by an order of magnitude depending on the combination of parameters, although none of them were implausible.
오상진(Sangjin Oh),이지홍(Jihong Lee),최훈(Hoon Choi) 제어로봇시스템학회 2008 제어·로봇·시스템학회 논문지 Vol.14 No.10
This article presents an omni-tread snake robot that designed to locomote on narrow space and rough terrain. The omni-tread snake robot comprises three segment, which are linked to each other by 2 degrees of freedom joints for the pitch and yaw motion. Moving tracks on all four sides of each segment guarantee propulsion even when the robot rolls over. The 2 DOF joint are actuated by 2 servo motors which produce sufficient torque to lift the one leading or trailing segments up and overcome obstacles. This paper applies articulated steering technique to get omni-tread snake robot’s kinematics model.
Kim, Daesoo,Yoon, Taeho,Park, Sangjin,Shin, Soyoung,Ryu, Ji Heon,Oh, Seung M. The Electrochemical Society 2014 Journal of the Electrochemical Society Vol.161 No.14
<P>In order to address the role of aluminum dopant in spinel-structured lithium manganese oxide (Li<SUB>1.08</SUB>Mn<SUB>1.84</SUB>Al<SUB>0.08</SUB>O<SUB>3.96</SUB>, Al-doped LMO) electrode, high-temperature (60°C) charge/discharge cycling and storage experiments are carried out. The Al-doped LMO outperforms the un-doped counterpart with respect to capacity fading and Mn dissolution. However, the electrode polarization becomes substantial for the Al-doped LMO after the high-temperature storage. As the resistive component to be responsible for the electrode polarization, Al<SUB>2</SUB>O<SUB>3</SUB> and Al(OH)<SUB>x</SUB>F<SUB>3-x</SUB> are identified on the Al-doped LMO surface from X-ray photoelectron spectroscopy study. It is very likely that the doped Al ions dissolve into the electrolyte during the high-temperature storage but re-deposit as a form of Al<SUB>2</SUB>O<SUB>3</SUB> and Al(OH)<SUB>x</SUB>F<SUB>3-x</SUB> on the LMO surface. Due to a scavenging action of these Al species for hydrogen fluoride, Mn dissolution is suppressed to give an improved cycle and storage performance at elevated temperature.</P>
Ruminal impaction with an indigestible foreign body in long-tailed goral (Naemorhedus caudatus)
Sangjin Ahn,Woojin Shin,Yujin Han,Sohwon Bae,Chea-Un Cho,Sooyoung Choi,Yeonsu Oh,Jong-Taek Kim 한국동물위생학회 2023 韓國家畜衛生學會誌 Vol.46 No.2
A 6-year-old female long-tailed goral (Naemorhedus caudatus , goral) was admitted to the Gangwon wildlife medical rescue center due to severe starvation and hypothermia. The goral displayed clinical signs of severe weight loss, dehydration, lethargy, regurgitation, hypothermia, and bloating, with blood tests showing a decrease in albumin, calcium, glucose, and sodium. Supportive care was given through heating, oxygen supply, and fluid therapy, but the goral’s condition worsened, and the goral died three days after admission. Radiographic and computed tomography scans revealed the presence of an indigestible foreign body (IFB) in the rumen, which was confirmed during the necropsy. The cause of goral’s death was identified as chronic rumen impaction caused by the IFB (a ball of string), with other pathological findings including bloating, the presence of serous ascites, and congestion of digestive organs. Blood biochemical changes, clinical signs, and veterinary medical imaging diagnosis can be helpful in diagnosing ruminal impaction. This case report can provide valuable information for the diagnosis and treatment of ruminal impaction caused by IFB in endangered gorals, which may include rumenotomy or other surgical procedures.