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Transcriptional Profiling of the Trichoderma reesei Recombinant Strain HJ48 by RNA-Seq
Huang, Jun,Wu, Renzhi,Chen, Dong,Wang, Qingyan,Huang, Ribo The Korean Society for Microbiology and Biotechnol 2016 Journal of microbiology and biotechnology Vol.26 No.7
The ethanol production of Trichoderma reesei was improved by genome shuffling in our previous work. Using RNA-Seq, the transcriptomes of T. reesei wild-type CICC40360 and recombinant strain HJ48 were compared under fermentation conditions. Based on this analysis, we defined a set of T. reesei genes involved in ethanol production. Further expression analysis identified a series of glycolysis enzymes, which are upregulated in the recombinant strain HJ48 under fermentation conditions. The differentially expressed genes were further validated by qPCR. The present study will be helpful for future studies on ethanol fermentation as well as the roles of the involved genes. This research reveals several major differences in metabolic pathways between recombinant strain HJ48 and wild-type CICC40360, which relates to the higher ethanol production on the former, and their further research could promote the development of techniques for increasing ethanol production.
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Transcriptional Profiling of the Trichoderma reesei Recombinant Strain HJ48 by RNA-Seq
( Jun Huang ),( Renzhi Wu ),( Dong Chen ),( Qingyan Wang ),( Ribo Huang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.6
The ethanol production of Trichoderma reesei was improved by genome shuffling in our previous work. Using RNA-Seq, the transcriptomes of T. reesei wild-type CICC40360 and recombinant strain HJ48 were compared under fermentation conditions. Based on this analysis, we defined a set of T. reesei genes involved in ethanol production. Further expression analysis identified a series of glycolysis enzymes, which are upregulated in the recombinant strain HJ48 under fermentation conditions. The differentially expressed genes were further validated by qPCR. The present study will be helpful for future studies on ethanol fermentation as well as the roles of the involved genes. This research reveals several major differences in metabolic pathways between recombinant strain HJ48 and wild-type CICC40360, which relates to the higher ethanol production on the former, and their further research could promote the development of techniques for increasing ethanol production.
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Luminescent bacteria toxicity assay in the study of mercury speciation
J.M.Ribo,Yang, J.E.,Huang, P.M. 江原大學校 附設 環境硏究所 1991 環境硏究 Vol.8 No.-
The toxicities of solutions of 10 mercury compounds to luminescent bacteria were measured using the Microtox Toxicity Bioassay. The aim of this study was to assess the influence that the counter-ions have on the aquatic toxicity of mercury salts. The toxicities of these mercury compounds were very similar, except for mercurous tannate and mercuric salicylate. This can be attributed to differences in the ionization and speciation patterns of these compounds relative to the other compounds tested. In general, the toxicity of the solutions at pH 5 was mot significantly different from the toxicity of these solutions at pH 6, but a clear reduction in toxicity was observed when the pH of the solution was adjusted to pH 9. Significant differences were found between the toxicity of Hg(I) and Hg(II) salts of the same anion at pH 9. When cysteine was added to a mercuric nitrate solution(at pH 6), a reduction in the toxicity was observed. This can be explained in terms of the strong binding of mercury to cysteine, thus reducing the concentration of mercury species available to cause an observable toxic effect to the bioluminescent bacteria.
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( Zhong Cheng ),( Dong Chen ),( Bo Lu ),( Yutuo Wei ),( Liang Xian ),( Yi Li ),( Zhenzhen Luo ),( Ribo Huang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.6
Acidic endo-polygalacturonases are the major part of pectinase preparations and extensively applied in the clarification of fruits juice, vegetables extracts, and wines. However, most of the reported fungal endo-polygalacturonases are active and stable under narrow pH range and low temperatures. In this study, an acidic endo-polygalacturonase (EPG4) was purified and characterized from a mutant strain of Penicillium oxalicum. The N-terminal amino acid sequence of EPG4 (ATTCTFSGSNGAASASKSQT) was different from those of reported endopolygalacturonases. EPG4 displayed optimal pH and temperature at 5.0 and 60-70°C towards polygalacturonic acid (PGA), respectively, and was notably stable at pH 2.2-7.0. When tested against pectins, EPG4 showed enzyme activity over a broad acidic pH range (>15.0% activity at pH 2.2-6.0 towards citrus pectin; and >26.6% activity at pH 2.2-7.0 towards apple pectin). The Km and Vmax values were determined as 1.27 mg/ml and 5,504.6 U/mg, respectively. The enzyme hydrolyzed PGA in endo-manner, releasing oligo-galacturonates from PGA, as determined by TLC. Addition of EPG4 (3.6 U/ml) significantly reduced the viscosity (by 42.4%) and increased the light transmittance (by 29.5%) of the papaya pulp, and increased the recovery (by 24.4%) of the papaya extraction. All of these properties make the enzyme a potential application in the beverage industry.
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Naikun Shen,Qingyan Wang,Yan Qin,Jin Zhu,Qixi Zhu,Huizhi Mi,Yutuo Wei,Ribo Huang 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.6
A method combining a Plackett-Burman design(PBD), the steepest ascent method (SA), and a Box-Behnken design (BBD) was developed to optimize succinicacid production from cane molasses by Actinobacillussuccinogenes GXAS137. The important parameters were(g/L): total sugars of cane molasses (85 g/L), yeast extract(8.84 g/L), and MgCO3 (63.1 g/L). Verification experimentsindicated that the maximal succinic acid productionreached 57.43±0.86 g/L, which agreed with the predictedvalue (57.12 g/L). In addition, batch and fed-batchfermentations were carried out in a 1.3 L stirred bioreactor. Compared with a batch fermentation that produced 57.96g/L of succinic acid at 60 h, a fed-batch fermentation,performed to minimize the inhibition effect of the substrate,produced 64.34 g/L of succinic acid at 60 h. The combinedmethod is powerful for selection of optimized conditionsfor succinic acid production from cane molasses.
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