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( Quanquan Liu ),( Pengfei Ren ),( Yang Liu ),( Wen Qin ),( Huayue Li ),( Wenli Li ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.6
Glycosyltransferases (GTs) from microbes are an emerging and rich source for efficient glycol-transformation of natural/unnatural compounds. Here, we probed the catalytic capability and substrate promiscuity of BmmGT1 from marine-derived Bacillus methylotrophicus B-9987. The regioselectivity of BmmGT1 on macrolactin A (1) was explored by optimization of the reaction conditions, in which a series of O-glycosylated macrolactins (1a-1e) were generated, including two new di/tri-O-glucosyl analogs (1b and 1e). Furthermore, BmmGT1 was able to catalyze the glycosylation of the thiol (S-) or amine (N-) sites of phenolic compounds (2 and 3), leading to the generation of N- (2a) or S-glycosides (3a and 3b). The present study demonstrates that BmmGT1 could serve as a potential enzyme tool for O-, N-, or S-glycosyl structural diversification of compounds for drug discovery.
Hui Dong,Quanquan Liu,Lina Xie,Bin Cong,Huan Wang 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.3
Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae) are haplo-diploid egg parasitoid wasps widely used as biological control agents against the Asian corn borer, Ostrinia furnacalis Guenée (Lepidoptera: Pyralidae). Two reproductive modes have been found in this species. Typically, female wasps produce haploid male offspring from unfertilized eggs and diploid female offspring from fertilized eggs (bisexual). However, they can also produce only diploid females when they are infected with Wolbachia, though the eggs are unfertilized (thelytokokous). To investigate the potential of a thelytokous Wolbachia-infected (TdW+) and a bisexual uninfected (Td) T. dendrolimi strain as inundative biocontrol agents, the functional responses of both strains toward their natural enemy (Asian corn borer) were evaluated at three constant temperatures (20, 25 and 30 °C). The results revealed two types of functional response for both strains: type II and III. TdW+strain displayed type II at 30 °C (high temperature) and III at 20 °C (low temperature) and 25 °C, while Td strain displayed type II at 20 °C and III at 25 °C and 30 °C. The comparison of functional response between two strains indicated TdW+ and Td strain had no significant difference at 20 °C. However, the estimated host handling time of the TdW+ strain was significantly shorter than that of the Td strain at 25 °C, and TdW+ strain had significantly higher instantaneous search rate and shorter handling time than Td strain at 30 °C. Shorter host handling time and higher instantaneous search rate in thelytokous Wolbachia-infected T. dendrolimi showed that this strain might be more effective for controlling Asian corn borer.
Jin-cheng Zhou,Quanquan Liu,Yong-xu Han,Hui Dong 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.3
Due to ongoing climate change, short-term extreme heat waves in the summer are expected to be more frequent. Insect eggs are sensitive to thermal stress. This raises the question of whether herbivore insects' thermal adaptability would be changed after a single extreme heat wave at the egg stage. In this study, we examined the developmental performance of Ostrinia furnacalis Guenée at 25 °C, 27 °C, 29 °C or 31 °C after a single extreme heat wave (42 °C) for 0 h (control), 1 h, 2 h, or 3 h at the egg stage. The results showed that O. furnacalis at the egg or larval stage was more sensitive to a single heat wave than it was at the pupae or adult stage. After a single heat wave, O. furnacalis showed a reduced egg-hatching rate or reduced larval survival rate, but the optimum temperature for egg hatching and larval survival was higher than that in the control. The upper temperature threshold and optimum temperature for larval development in the control were higher than that after a single extreme heat wave. Both male and female pupal weight decreased with increasing temperature, and pupal weight decreased faster in females than in males. The Cox proportional hazard model showed that when O. furnacalis developed at 25 °C, the instantaneous death risk of adults with a 3 h heat wave at the egg stage was higher than that of the control, but when O. furnacalis developed at 29 °C and 31 °C, the instantaneous death risk of adults after a heat wave was significantly lower than that of the control. Our study highlights the effect of a single heat wave on O. furnacalis eggs and the subsequent development of survival individuals.
Jing Ye,Yasutaka Nakashima,Takao Watanabe,Masatoshi Seki,Bo Zhang,Quanquan Liu,Yuki Yokoo,Yo Kobayashi,Qixin Cao,Masakatsu G. Fujie 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10
In consideration of a large proportion of stroke survivors with persistent deficits, current interventions have limited ability to restore normal motor function. We propose a gait training system for gait rehabilitation of hemiplegic patients with convalescent stroke. It consists of a functional electrical stimulation (FES) device which is used to influence the gait pattern instantly to improve the quality of the gait, and a treadmill with two separated belts. The stimulus triggers from the FES could be controlled automatically by the subject`s gait phase determination, including stance phase and swing phase. Gait phase could be estimated and recognized through observing current value variation of the treadmill motor during the subject’s walking on the treadmill belts. In this paper, we have preliminarily tested the feasibility of the proposed method through hemiplegic simulation experiments. Also, Tibialis Anterior (TA) and quadriceps on the hemiplegic side were successfully stimulated by the expected FES stimuli during gait.
Jin-cheng Zhou,Qian-jin Dong,Tong-shu Zhang,Li-jia Duan,Su-fang Ning,Quanquan Liu,Yuan-yuan Li,Chun-xue Li,Hui Dong 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3
The efficacy of Trichogramma against pests basically depends on the dispersal capacity of the wasps. A releaserecapture method was used in this study to evaluate the effect of relative wind speed (RWS) during the nighttime and the daytime on the dispersal capacity of postrelease population of Trichogramma dendrolimi Matsumura. Two repeated measurements were carried out, using sentinel cards with the host eggs and yellow sticky card traps on the third and the sixth day after wasp release. The results showed that: (1) both the number of recaptured wasps and parasitized eggs increased with the RWS in the daytime during the first three days. The number of parasitized eggs also increased with the RWS in the daytime over the latter three days. The distances that encompassed 98% recaptured T. dendrolimi adults (X 98 ) at the first three days was quadratically increased by the RWS of the daytime. (2) The number of wasps recaptured decreased with the distance from the releasing point during the first three days, but it was not influenced by the distance from the releasing point during the latter three days. (3) The average dispersal radius of the wasps during the first three days was shorter than during the latter three days. The present results will be helpful to improve release techniques based on the wind effects at different times.