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RISS 인기검색어
- 검색키워드
- 저자 : ( Partha Deb Ghosh ) (검색결과 3 건)
- 무료
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Soumen Saha,Chandan Sengupta,Partha Deb Ghosh 한국작물학회 2014 Journal of crop science and biotechnology Vol.17 No.4
Genetic uniformity is one of the most important prerequisites for successful micropropagation of crop species. Genetic fidelity ofin vitro raised 40 plants of Ocimum basilicum L. derived from nodal explants was assessed by 56 RAPD and 17 ISSR markers, fortheir genetic stability. Out of 56 RAPD and 17 ISSR primers screened, only 31 RAPD and 11 ISSR primers produced clear, reproducible,and scorable bands. The 31 RAPD primers produced 92 distinct and scorable loci, with an average of 2.96 loci per primer. The number of scorable loci for ISSR primers varied from three (ISSR-BG09 and ISSR-BG11) to eight (ISSR-BG01), with an averageof 4.81 loci per primer. The number of loci generated per primer was greater in ISSR than RAPD. The markers designed fromAG motif amplified more number of loci. The markers anchored at 3´ ends produced a higher number of consistent bands than unanchoredmarkers. All banding profiles from micropropagated plants were monomorphic and similar to those of the mother plant. Asimilarity matrix based on Jaccard’s coefficient revealed that the pair-wise value between the mother and the in vitro-raised plantletswas 1, indicating 100% similarity. This confirmed the true-to-type nature of the in vitro-raised clones
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Vijayan, Kunjupillai,Chakraborti, Shyama Prasad,Doss, Subramaniam Gandhi,Ghosh, Partha Deb,Ercisli, Sezai Korean Society of Sericultural Science 2008 International Journal of Industrial Entomology Vol.16 No.2
A line x tester analysis was carried out in mulberry (Morns spp.) under different salinity levels to determine the changes in the genetic interaction of various morpho-biochemical characters. Five mulberry genotypes, 3 females and 2 males, differing in salt tolerance were selected for the study. Clones of these parents along with clones of the F1 hybrids were planted in earthen pots and subjected to different levels of salinity (0.0%, 0.25%, 0.50%, 0.75% and 1.00% NaCl). Data on morphological and biochemical characters were subjected to line x tester analysis. The result revealed significant variation among the parents studied. The prominence of non-additive gene effect under control condition suggests the need for well chalked out breeding program to exploit the non-fixable variance of components for improvement of plant height, leaf size and leaf yield, chlorophyll and photosynthesis in mulberry. However, under salinity stress a shift from non-additive gene effect to additive gene effect for the above said character further suggests the need for a change in breeding strategy. The general combining ability (GCA) analysis has identified English black as the best combiner among the parents and the specific combining ability analysis (SCA) found crosses of English black X C776 and Rotndiloba x Mandalaya were good for Plant height and leaf size and English black X C776 and Rotundiloba x C776 were good for biochemical proline and chlorophyll. From the performance of parents and their crosses under different salinity levels and also under normal cultural conditions it is concluded that in mulberry different approaches are required to develop varieties for the irrigated and saline conditions.
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( Kunjupillai Vijayan ),( Shyama Prasad Chakraborti ),( Subramaniam Gandhi Doss ),( Partha Deb Ghosh ),( Sezai Ercisli ) 한국잠사학회 2008 International Journal of Industrial Entomology Vol.16 No.2
A line x tester analysis was carried out in mulberry (Morus spp.) under different salinity levels to determine the changes in the genetic interaction of various morpho-biochemical characters. Five mulberry genotypes, 3 females and 2 males, differing in salt tolerance were selected for the study. Clones of these parents along with clones of the F1 hybrids were planted in earthen pots and subjected to different levels of salinity (0.0%, 0.25%, 0.50%, 0.75% and 1.00% NaCl). Data on morphological and biochemical characters were subjected to line x tester analysis. The result revealed significant variation among the parents studied. The prominence of non-additive gene effect under control condition suggests the need for well chalked out breeding program to exploit the non-fixable variance of components for improvement of plant height, leaf size and leaf yield, chlorophyll and photosynthesis in mulberry. However, under salinity stress a shift from non-additive gene effect to additive gene effect for the above said character further suggests the need for a change in breeding strategy. The general combining ability (GCA) analysis has identified English black as the best combiner among the parents and the specific combining ability analysis (SCA) found crosses of English black X C776 and Rotndiloba x Mandalaya were good for Plant height and leaf size and English black X C776 and Rotundiloba x C776 were good for biochemical proline and chlorophyll. From the performance of parents and their crosses under different salinity levels and also under normal cultural conditions it is concluded that in mulberry different approaches are required to develop varieties for the irrigated and saline conditions.
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