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Generation of Transgenic Pigs Carrying a Construct for Endothelial Specific Expression of CD73
Keon Bong Oh,Bella Kim,Nayoung Ko,Dae-Jin Kwon,Dong-Hoon Kim,Gi-Sun Im,Jin-Ki Park,Seongsoo Hwang 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1
Despite of the absence of hyperacute rejection and acute humoral xenograft rejection, the organ graft of the a1,3-galactosyltransferase (GalT) gene knockouted (KO) and complement regulatory protein (CRP) expressing pig into a nonhuman primate is rejected by development of a thrombotic microangiopathy and/or a consumptive coagulopathy. Thus further introduction of genes to overcome the coagulation incompatibilities between pig and primate under GalT KO/CRP genetic background has been strongly suggested. CD73 (ecto-5'-nucelotidase) is an enzyme attached via a glycosyl phosphoinositol anchor to the extracellular membrane of endothelial cells, which catalyses the hydrolysis of adenosine triphosphate to adenosine. Loss of activity of CD73 results in activation and aggregation of platelets by a reduced capacity to convert nucleotides to adenosine. In previous study, we reported generation of GalT KO fibroblasts concurrently expressing membrane cofactor protein and produced cloned pigs by nuclear transfer of the fibroblast cells (1). In this study, we constructed a vector for expression of human CD73 under control of promoter of pig Icam2 gene expressed specifically at endothelial cells. This vector was introduced into porcine fibroblasts using the nucleofection technology, by which we had forty three fibroblasts clones carrying pIcam2- CD73 vector. Somatic cell nuclear transfer resulted in generation of two transgenic piglets survived.
( Nayoung Ko ),( Myeonggi Hong ),( Jeeseon Hwang ),( Jeonghyeon Chang ),( Euigab Hwang ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.8
This study examines the similarities and differences between the causes of juvenile delinquency in online and offline environments and in personal characteristics. The study utilizes data from the ‘2014 Survey on Juvenile Victimization in Korea’. The population of this survey is students attending middle and high schools across the country. While this paper is based on the Self-Control theory, opportunity factors based on the Routine Activity theory and the Situational Action theory are also applied. Results show that the causes of offline delinquency are low self-control, routine activity and frequent gaming and SNS use. The causes of online delinquency are high self-control, existence of communication with unknown persons and the spectrum of personal information online. The common element of offline and online delinquency was the presence of delinquent peers. These results show that while online and offline delinquency cannot be explained with the same methodology, at the same time they are not mutually exclusive.
Nayoung Ko,Bella Kim,Seong-soo Hwang,Jeong Woong Lee,Dong-Hoon Kim,Gi-Sun Im,Byoung-Chul Yang,Man-Jong Kang,Choon-Keun Park,Jin-Ki Park,Keon Bong Oh 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1
Immunological rejection of the organ grafted onto a primate arises from two antibody mediated processes, hyperacute rejection (HAR) and acute humoral rejection (AHR). Functional ablation of α1,3-galactosyltransferase (GalT) and concurrently overexpression of complement regulatory proteins are known to inhibit HAR and AHR. In previous study, we reported that production of porcine male fibroblasts harboring a MCP expression cassette targeted to GalT locus. In this study, we constructed a different MCP expression cassette, in which the EF1α promoter regulates MCP expression and internal ribosome entry site-mediated neomycin resistance gene expression. Subsequently, this cassette was inserted between the left and the right homologous arms to target exon 9 of the GalT gene. Female fibroblasts were isolated from ear skin of 10 days old miniature pig, and used for nucelofection of the the construct for MCP expression at GalT locus. PCR analysis showed that four clones of forty neomycin resistant clones carry MCP expression cassette at exon 9 of the GalT gene. Two clones analyzed downregulated GalT expression, as determined by quantitative reverse transcriptase polymerase chain reaction. Flow cytometry analysis showed that MCP was efficiently expressed at the cell surface.
Ko, Nayoung,Lee, Jeong-Woong,Hwang, Seong Soo,Kim, Bella,Ock, Sun A,Lee, Sung-Soo,Im, Gi-Sun,Kang, Man-Jong,Park, Jin-Ki,Oh, Sung Jong,Oh, Keon Bong Marcel Dekker 2013 Animal biotechnology Vol.24 No.4
<P>Xenotransplantation of pig organs into primates leads to hyperacute rejection (HAR). Functional ablation of the pig α 1,3-galactosyltransferase (GalT) gene, which abrogates expression of the Gal α 1-3Gal β 1-4GlcNAc-R (Gal) antigen, which inhibits HAR. However, antigens other than Gal may induce immunological rejection by their cognate antibody responses. Ultimately, overexpression of complement regulatory proteins reduces acute humoral rejection by non-Gal antibodies when GalT is ablated. In this study, we developed a vector-based strategy for ablation of GalT function and concurrent expression of membrane cofactor protein (MCP, CD46). We constructed an MCP expression cassette (designated as MCP-IRESneo) and inserted between the left and the right homologous arms to target exon 9 of the GalT gene. Nucleofection of porcine ear skin fibroblasts using the U-023 and V-013 programs resulted in high transfection efficiency and cell survival. We identified 28 clones in which the MCP-IRESneo vector had been successfully targeted to exon 9 of the GalT gene. Two of those clones, with apparent morphologically mitotic fibroblast features were selected through long-term culture. GalT gene expression was downregulated in these 2 clones. Importantly, MCP was shown to be efficiently expressed at the cell surface and to efficiently protect cell lysis against normal human complement serum attack in vitro.</P>
One-to-One 가시광 통신 기반 IoT 모니터링 시스템
고나영(Nayoung Ko),최다봄(Dabom Choi),김동훈(Donghoon Kim),심규성(Kyusung Shim),안병구(Beongku An) 한국통신학회 2022 한국통신학회 학술대회논문집 Vol.2022 No.2
본 논문에서는 One-to-One 가시광 통신 기반 IoT 모니터링 시스템을 제안한다. 제안한 시스템은 시스템 스스로 IoT 장치와 환경 센서를 이용하여 내부와 외부 환경을 일정 시간마다 측정하고 이를 취합하여 클라우드 서버로 전송한다. 클라우드 서버는 이를 시각화하여 사용자와 관리자에게 실시간으로 특정 공간의 현재 상태를 파악할 수 있도록 한다. 성능평가를 통하여, 구현한 IoT 시스템이 일정 시간마다 실외 자외선 지수와 실내 온도 습도를 측정하고 이를 클라우드 서버로 전송하여 시각화함을 확인하였다. 현재 본 연구진들은 구현된 결과에 머신러닝을 적용하여 실시간으로 IoT 시스템 스스로 판단하고 동작하는 스마트 IoT 시스템으로 개발과 Multi-to-One 가시광 통신시스템으로의 확장을 진행 중이다.