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- 저자 : ( Mi Song Fei Yu ) (검색결과 2 건)
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Yu-Guo Yuan,Shao-Zheng Song,Meng-Ming Zhu,Zheng-Yi He,Rui Lu,Ting Zhang,Fei Mi,Jin-Yu Wang,Yong Cheng 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.8
Objective: To create genetically modified goat as a biopharming source of recombinant human lacotoferrin (hLF) with transcription activator-like effector nucleases. Methods: TALENs and targeting vector were transferred into cultured fibroblasts to insert hLF cDNA in the goat beta-lactoglobulin (BLG) locus with homology-directed repair. The gene targeted efficiency was checked using sequencing and TE7I assay. The bi-allelic gene targeted colonies were isolated and confirmed with polymerase chain reaction, and used as donor cells for somatic cell nuclear transfer (SCNT). Results: The targeted efficiency for BLG gene was approximately 10%. Among 12 Bi-allelic gene targeted colonies, five were used in first round SCNT and 4 recipients (23%) were confirmed pregnant at 30 d. In second round SCNT, 7 (53%), 4 (31%), and 3 (23%) recipients were confirmed to be pregnant by ultrasound on 30 d, 60 d, and 90 d. Conclusion: This finding signifies the combined use of TALENs and SCNT can generate bi-allelic knock-in fibroblasts that can be cloned in a fetus. Therefore, it might lay the foundation for transgenic hLF goat generation and possible use of their mammary gland as a bioreactor for large-scale production of recombinant hLF.
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An ID-based Broadcast Encryption Scheme for Cloud-network Integration in Smart Grid
( Shufen Niu ),( Lizhi Fang ),( Mi Song Fei Yu ),( Song Han ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.9
The rapid growth of data has successfully promoted the development of modern information and communication technologies, which are used to process data generated by public urban departments and citizens in modern cities. In specific application areas where the ciphertext of messages generated by different users’ needs to be transmitted, the concept of broadcast encryption is important. It can not only improve the transmission efficiency but also reduce the cost. However, the existing schemes cannot entirely ensure the privacy of receivers and dynamically adjust the user authorization. To mitigate these deficiencies, we propose an efficient, secure identity-based broadcast encryption scheme that achieves direct revocation and receiver anonymity, along with the analysis of smart grid solutions. Moreover, we constructed a security model to ensure wireless data transmission under cloud computing and internet of things integrated devices. The achieved results reveal that the proposed scheme is semantically secure in the random oracle model. The performance of the proposed scheme is evaluated through theoretical analysis and numerical experiments.
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