흑연료 원자흡수 분광법에 의한 혈중의 납, 카드뮴 정량을 위한 외부정도관리 시료제조 및 분석

이공주,임홍빈 이화여자대학교 생명과학연구소 1995 생명과학연구논문집 Vol.6 No.-

납과 카드뮴을 포함하는 여러 가지 농도의 동결건조된 혈액이 외부정도관리 시료로서 제조되었다. 이 시료들은 흑연료 원자흡수분광법(GFAAS)을 이용하여 성능이 파악되었다. 매트릭스 개선제로서 0.1% ammonium dihydrogen phosphate와 0.1% Triton X-100을 사용하여 섭씨 600내지 650도의 희화온도에서 혈액에 있는 납과 카드뮴의 정량 분석을 위한 GFAAS의 최적 분석조건이 얻어졌다. 제조된 혈액의 균질도와 안정도는 최적화된 분석조건에서 연구되었다. Lyophilized whole blood samples containing various concentrations of Pb and Cd have been prepared as external quality control materials. These materials have been characterized with graphite furnace atomic absorption spectrometry(GFAAS). The optimized obtained at the ashing temperature of 600~650℃ with 0.1% ammonium dihydrogen phosphate and 0.1% Triton X-100 as matrix modifier. Homogeniety and stability of the prepared whole blood have been studied at the optimized analytical condition.

슬러지 재자원화에 관한 연구

이주성,공성호,서승원,배성렬,김영채,이기철 漢陽大學校 環境工學硏究所 1997 環境科學論文集 Vol.18 No.-

이 연구는 1993년도에 우리나라의 연간 슬러지발생량이 약 3500만m³에 이르고 있으며, 이들 대부분이 매립되고 있다. 그러나 매립부지의 확보가 점점 어려워 지고 있으며 아울러 매립처분비용도 상승하고 있기 때문에 oil화, 퇴비화등으로 재자원화 하는 방법과 소각후의 소각회를 유효이용하는 방법 등이 연구 개발되고 실용화되고 있다. 슬러지의 oil화는 현재 연구단계에 있으나 앞으로 슬러지의 유효이용기술로서, 가장 좋은 방법이 되리라 기대되고 있다. 그러나 소각후의 소각회의 이용방법은 현재 일본등에서는 실용화 되어 건설자재 등으로 다양하게 이용하고 있다. 우리나라에서도 서울시 등을 비롯한 대도시에서는 하수처리장에서 발생하는 슬러지를 소각한 후에 소각회를 건설 자제용으로서 지자체의 공사에는 반드시 사용하도록 하는 제도를 만든다면 유효이용방법으로 충분한 타당성이 있을 것이다. 이때 소각방법으로는 용융소각방법이 보다 효과적일 것이다. With increasing sewage and wastewater sludge, it has become difficult to dispose the sludge by landfilling and ocean dumping. To solve this problem, efforts have been made to delelop sewage and wastewater sludge utilization technologies in many advanced countries. Some technologies have already been developed; conversion of sludge to compost and fuel, producing artificial light-weight aggregate from ashes. This paper describes the technical status of sludge utilization technologies.

흑연료 원자흡수 분광법에 의한 혈중의 납, 카드뮴 정량을 위한 외부정도관리 시료제조 및 분석

이공주,임홍빈 梨花女子大學校 藥學硏究所 1995 藥學硏究論文集 Vol.- No.5

납과 카드늄을 포함하는 여러가지 농도의 동결건조된 혈액이 외부정도관리 시료로서 제조되었다. 이 시료들은 흑연료 원자흡수분광법(GFAAS)을 이용하여 성능이 파익되었다. 매트릭스 개선제로서 0.1% ammonium dihydrogen phosphate와 0.1% Triton X-100을 사용하여 섭씨 600 내지 650도의 회화온도에서 혈액에 있는 납과 카드늄의 정량분석을 위한 GFAAS의 최적 분석조건이 얻어졌다.제조된 혈액의 균질도와 안정도는 분석조건에서 연구되었다. Lyophilized whole blood samples containing various concentrations of Pb and Cd have been prepared as external quality control materials. These materials have been characterized with graphite furnace atomic absorption spectrometry(GFAAS). The optimized conditions for the quantitative determination of Pb and Cd in whole blood using GFAAS were obtained at the ashing temperature of 600∼650℃, with 0.1% ammonium dihydrogen phosphate and 0.1% Triton X-100 as matrix modifier. Homogeniety and stability of the prepared whole blood have been studied at the optimized analytical condition.

모세관 전기영동 분석법의 복합약물제제의 품질관리 분석에 응용을 위한 연구

허유정,이공주 梨花女子大學校 藥學硏究所 1998 藥學硏究論文集 Vol.- No.7

Capillary electrophoresis (CE) is perceived as an attractive tool for the analysis of pharmaceuticals and biological materials because of their high separation efficiency. easy separation and low running cost. New concept of micellar electrokinetic capillary chromatography (MECC) expanded the application of CE to the separation of neutral molecules. Validation of CE as an analytical technique for quality control of pharmaceuticals should be confirmed by quantitative analysis and the peak confirmation. In this study, the quantitative analyses of various types of neutral. acidic and basic components (acetaminophen. caffeine. ascorbic acid. riboflavin. thiamine. chlorpheniramine. phenylpropanolamine. dl-methylephedrine and dextromethorphan) in complex cold medicines have been accomplished using CE. Combined methods of MECC using SDS and capillary zone electrophoresis lowering the pH of running buffer were adopted to determine the ingredients in capsule type or liquid formula complex medicines without particular sample pretreatment. The results indicate that CE is a promising technique for quality control analysis of pharmaceuticals as a validation method.

Analysis of Double-Stranded DNA Fragments by Capillary Electrophoresis Using Entangle Polymer Solutions in Uncoated Fused Silica Capillary Columns

Lee,Kong-Joo,Lee,Jong Jin The Korea Science and Technology Center 1998 BMB Reports Vol.31 No.4

DNA fragments (51-587 bp) were separated by capillary electrophoresis using entangled polymer, hydroxyethylcellulose, in uncoated fused silica capillary colmns. The factors affecting the separation of DNA fragments with hydroxyethylcellulose media were evaluated, i.e., the concentration of buffer and entangled polymer, effects of additives (methanol, ethidium bormide, EDTA), temperature, and injection methods. Maximum performance was obtained by adding 5% methanol in 0.5% hydroxyethylcellulose solution at 30℃. Addition of methanol in polymer media increased the resolution of small size DNA fragments(<100 bp). On the other hand, addition of ethidium bromide and EDTA, which are commonly used in conventional DNA separation, reduced the resolution of DNA fragments in the polymer solution. It turns out that the separation behavior of DNA in entangled polymer is more sensitive to the running condition compared to that in polyacrylamide gel-filled capillary, but the reproducibility of DNA separation in entangled polymer is reliable.

New Algorithm for the Identification of Intact Disulfide Linkages Based on Fragmentation Characteristics in Tandem Mass Spectra

Lee, Kong Joo,Choi, Seon Hwa,Jeong, Jae Ho,Na, Seung Jin,Lee, Hyo Sun,Kim, Hwa Youn,Paek, Eun Ok 梨花女子大學校 藥學硏究所 2011 藥學硏究論文集 Vol.- No.21

Identifying the sites of disulfide bonds in a protein is essential for thorough understanding of a protein's tertiary and quaternary structures and its biological functions. Disulfide linked peptides are usually identified indirectly by labeling free sulfhydryl groups with alkylating agents, followed by chemical reduction and mass spectral comparison or by detecting the expected masses of disulfide linked peptides on mass scan level. However, these approaches for determination of disulfide bonds become ambiguous when the protein is highly bridged and modified. For accurate identification of disulfide linked peptides, we present here an algorithmic solution for the analysis of tandem mass (MS/MS) spectra of disulfide bonded peptides under nonreducing condition. A new algorithm called "DBond" analyzes disulfide linked peptides based on specific features of disulfide bonds. To determine disulfide linked sites, DBond takes into account fragmentation patterns of disulfide linked peptides in nucleoside diphosphate kinase (NDPK) as a model protein, considering fragment ions including cysteine, cysteine thioaldehyde (-2 Da, C(T)), cysteine persulfide (+32 Da, C(S)) and dehydroalanine (-34 Da, C(Delta)). Using this algorithm, we successfully identified about a dozen novel disulfide bonds in a hexa EF-hand calcium binding protein secretagogin and in a methionine sulfoxide reductase. We believe that DBond, taking into account the disulfide bond fragmentation characteristics and post-translational modifications, offers a novel approach for automatic identification of unknown disulfide bonds and their sites in proteins from MS/MS spectra.

Can Korean Functional Suffixes Trigger Sentiment in Short Reviews?

Kong Joo Lee(이공주),Jee Eun Kim(김지은) 한국언어학회 2021 언어 Vol.46 No.4

Grammatical categories contributed to sentiment analysis have been mostly a word or a bag of words which express semantic orientation. Korean, however, presents some of the sentence-final suffixes to be exploited for identifying sentiment. Those suffixes mark honorification which are interpreted as lowered or raised speech depending on their type. This research has referred them as Sentiment-Triggering Suffixes (STSs) and extracted them from a large sentiment corpus, utilizing Chi-square test and Association rules. A series of experiments has proved that using STSs as a feature is effective in detecting sentiment particularly when a sentence is short in length and does not contain a sentiment-bearing word. In addition, better results have been derived when STSs imposing negative sentiment were implemented. Utilizing STSs, in particular, helps identify rhetorical question, which is considered as a challenging task. Since cutting-edge machine learning techniques can visualize the most important features of a classifier, we have illustrated the characteristics of STSs using two of the techniques, t-SNE and heatmap.

IDENTIFICATION of SIGNALING MOLECULES IN STRESS-INDUCED APOPTOSIS BY PROTEOMICS

Lee, Kong-Joo 이화여자대학교 세포신호전달연구센터 2001 고사리 세포신호전달 심포지움 Vol. No.3

The exposure of cells to various environmental stresses such as heat shock and oxidant, hypoxia, death surface receptors and various chemicals, can induce apoptotic cell death. However, it is suggested that the apoptotic signaling pathways in response to various stresses are different. We investigated the cellular changes by heat shock, oxident(H₂O₂ and diamide) and death surface receptors. Differentially expressed proteins, modified proteins, and protein-protein interaction in various cells in response to stresses were identified using 2-dimensional gel electrophoresis and MALDI-TOF-MS. The functional studies were performed by biochemical methods. Combination of biochemical methods with proteomics makes it possible to identify the new signaling pathways ways.

A New Double-Stranded RNA Mycovirus from Pleurotus ostreatus (ASI 2504)

Lee, Jin-Kyung,Lee, Kang-Hyo,Shim, Hye-Kyung,Yang, Joo-Sung,Kim, Gyu-Hyun,Kong, Won-Sik,Yoo, Young-Bok,Kim, Dae-Hyun,Kim, Dong-Giun,Lee, Suk-Chan The Korean Society of Plant Pathology 2006 Plant Pathology Journal Vol.22 No.1

A new virus with a dsRNA genome was isolated and characterized from the Suhan-:neutari strain (ASI 2504) of Pleurotus ostreatus, which was characterized as long and slightly bent with small caps on the stipe of fruit body. Thirty nm isometric viruses with three dsRNA segments (approximately 2.0, 1.84 and 1.82 kb in sizes) were isolated by ultracentrifugation in sucrose gradients. Western analysis of protein extracted purified viruses with anti-virus polyclonal antibody confirmed that viruses have two specific proteins (36 and 68 kDa). Computer analysis of 2.0 kb segment shows that high. sequence identity with RNA-dependent RNA polymerase (RdRp) of partitiviruses, respectively. When compared to other dsRNA mycoviruses in a phylogenetic analysis, OMDV was most related to Pleurotus ostreatus virus 1.

Sulfhydryl-Specific Probe for Monitoring Protein Redox Sensitivity

Lee, Jae-Jin,Ha, Sura,Kim, Hee-Jung,Ha, Hyun Joo,Lee, Hee-Yoon,Lee, Kong-Joo American Chemical Society 2014 ACS CHEMICAL BIOLOGY Vol.9 No.12

<P>Reactive oxygen species (ROS) regulate various biological processes by modifying reactive cysteine residues in the proteins participating in the relevant signaling pathways. Identification of ROS target proteins requires specific reagents that identify ROS-sensitive cysteine sulfhydryls that differ from the known alkylating agents, iodoacetamide and <I>N</I>-ethylmaleimide, which react nonspecifically with oxidized cysteines including sulfenic and sulfinic acid. We designed and synthesized a novel reagent, methyl-3-nitro-4-(piperidin-1-ylsulfonyl)benzoate (NPSB-1), that selectively and specifically reacts with the sulfhydryl of cysteines in model compounds. We validated the specificity of this reagent by allowing it to react with recombinant proteins followed by peptide sequencing with nanoUPLC-ESI-q-TOF tandem mass spectrometry (MS/MS), and mutant studies employed it to identify cellular proteins containing redox-sensitive cysteine residues. We also obtained proteins from cells treated with various concentrations of hydrogen peroxide, labeled them with biotinylated NPSB-1 (NPSB-B), pulled them down with streptavidin beads, and identified them with MS/MS. We grouped these proteins into four families: (1) those having reactive cysteine residues easily oxidized by hydrogen peroxide, (2) those with cysteines reactive only under mild oxidative stress, (3) those with cysteines reactive only after exposure to oxidative stress, and (4) those with cysteines that are reactive regardless of oxidative stress. These results confirm that NPSBs can serve as novel chemical probes for specifically capturing reactive cysteine residues and as powerful tools for measuring their oxidative sensitivity and can help to understand the function of cysteine modifications in ROS-mediated signaling pathways.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/acbcct/2014/acbcct.2014.9.issue-12/cb500839j/production/images/medium/cb-2014-00839j_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/cb500839j'>ACS Electronic Supporting Info</A></P>