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Khalid Abdallah Hussein,주진호 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.6
Recently, public concerns regarding the use of agrochemicals have increased due to the environmental impacts and potential risks to human health. The application of beneficial microorganisms is a novel technology to improve plant health and productivity and has therefore been extensively studied as an alternative strategy for biocontrol. In our study, 122 microbial isolates were obtained from the rhizosphere of Panax ginseng and subsequently tested in vitro for phosphate solubilization and indole acetic acid (IAA) production. Pikovskaya’s medium was used to estimate rhizomicrobial isolates to solubilize tricalcium phosphate [Ca3 (PO4)2]. Among all the investigated strains, 82 % of rhizospheric fungi showed phosphate solubilization activity; however, only 57.1 % of the rhizobacteria isolates showed phosphate solubilization ability. For IAA production, 64.7 % of the tested rhizofungi isolates were able to produce the phytohormone; however, only 47.62 % of the rhizobacteria isolates exhibited IAA production. Among all investigated species, Pseudomonas fluorescence and Azotobacter chroococcum showed the highest phosphate solubility demonstrating 885.4 and 863.4 lg mL-1, respectively. Mucor sp. produced 42.3 lg mL-1 of IAA in Czapek’s tryptophan medium, and the highest fungal species to solubilize the inorganic phosphate (237.5 lg mL-1) was estimated by Penicillium sp. Rhizobacteria were more effective than rhizofungi in phosphate solubilization and IAA production. This study introduces some potent species in terms of phosphate solubilization and IAA production which could be likely to improve soils’ quality and promote plant growth.
( Khalid Abdallah Hussein ),( Young-don Lee ),( Jin Ho Joo ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.7
Rosemary essential oil was evaluated for antifungal potentiality against six major ginseng pathogens: Sclerotinia sclerotiorum, Sclerotinia nivalis, Cylindrocarpon destructans, Alternaria panax, Botrytis cinerea, and Fusarium oxysporum. The in vitro fungicidal effects of two commonly used fungicides, namely mancozeb and fenhexamid, and the volatile organic compounds (VOCs) of Trichoderma koningiopsis T-403 on the mycelial growth were investigated. The results showed that rosemary essential oil is active against all of the pathogenic strains of ginseng root rot, whereas rosemary oil displayed high ability to inhibit the Sclerotinia spp. growth. The highest sensitivity was S. nivalis, with complete inhibition of growth at 0.1% v/v of rosemary oil, followed by Alternaria panax, which exhibited 100% inhibition at 0.3% v/v of the oil. Minimum inhibitory concentrations (MICs) of rosemary oil ranged from 0.1 % to 0.5 % (v/v). Chemical analysis using GC-MS showed the presence of thirty-two constituents within rosemary oil from R. officinals L. Camphore type is the most frequent sesquiterpene in rosemary oil composition. Mancozeb and fenhexamid showed their highest inhibition effect (45% and 30%, respectively) against A. panax. T. koningiopsis T-403 showed its highest inhibition effect (84%) against C. destructans isolate. This study may expedite the application of antifungal natural substances from rosemary and Trichoderma in the prevention and control of phytopathogenic strains in ginseng root infections.
Zinc Ions Affect Siderophore Production by Fungi Isolated from the Panax ginseng Rhizosphere
( Khalid Abdallah Hussein ),( Jin Ho Joo ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.1
Although siderophore compounds are mainly biosynthesized as a response to iron deficiency in the environment, they also bind with other metals. A few studies have been conducted on the impact of heavy metals on the siderophore-mediated iron uptake by microbiome. Here, we investigated siderophore production by a variety of rhizosphere fungi under different concentrations of Zn<sup>2+</sup> ion. These strains were specifically isolated from the rhizosphere of Panax ginseng (Korean ginseng). The siderophore production of isolated fungi was investigated with chrome azurol S (CAS) assay liquid media amended with different concentrations of Zn<sup>2+</sup> (50 to 250 μg/ml). The percentage of siderophore units was quantified using the ultra-violet (UV) irradiation method. The results indicated that high concentrations of Zn<sup>2+</sup> ion increase the production of siderophore in iron-limited cultures. Maximum siderophore production by the fungal strains was detected at Zn<sup>2+</sup> ion concentration of 150 μg/ml except for Mortierella sp., which had the highest siderophore production at 200 μg/ml. One potent siderophoreproducing strain (Penicillium sp. JJHO) was strongly influenced by the presence of Zn<sup>2+</sup> ions and showed high identity to P. commune (100% using 18S-rRNA sequencing). The purified siderophores of the Penicillium sp. JJHO strain were chemically identified using UV, Fouriertransform infrared spectroscopy (FTIR), and matrix-assisted laser desorption/ionization timeof- flight mass spectrometer (MALDI-TOF-MS) spectra.
( Khalid Abdallah Hussein ),( Jin Ho Joo ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.6
Salinity stress is an important environmental problem that adversely affects crop production by reducing plant growth. The impacts of rhizobacterial strains to alleviate salinity stress on the germination of Lactuca sativa and Raphanus sativus seeds were assessed using different concentrations of NaCl. Plant growth-promoting rhizobacteria (PGPR) strains were also examined to improve the early germination of Chinese cabbage seeds under normal conditions. Lactobacillus sp. and P. putida inoculation showed higher radicle lengths compared with non-inoculated radish (Raphanus sativus) seeds. LAP mix inoculation increased the radicle length of lettuce (Lactuca sativa) seedlings by 2.0 and 0.5 cm at salinity stress of 50 and 100 mM NaCl concentration, respectively. Inoculation by Azotobacter chroococcum significantly increased the plumule and radicle lengths of germinated seeds compared with non-inoculated control. A. chroococcum increased the radicle length relative to the uninoculated seeds by 4.0, 1.0, and 1.5 cm at 50, 100, and 150 mM NaCl concentration, respectively. LAP mix inoculation significantly improved the radicle length in germinated radish seeds by 7.5, 1.3, 1.2, and 0.6 cm under salinity stress of 50, 100, 150, and 200 mM NaCl concentration, respectively. These results of this study showed that PGPR could be helpful to mitigate the salinity stress of different plants at the time of germination.
Hussein, Khalid Abdallah,Joo, Jin Ho The Korean Society for Applied Biological Chemistr 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.6
Recently, public concerns regarding the use of agrochemicals have increased due to the environmental impacts and potential risks to human health. The application of beneficial microorganisms is a novel technology to improve plant health and productivity and has therefore been extensively studied as an alternative strategy for biocontrol. In our study, 122 microbial isolates were obtained from the rhizosphere of Panax ginseng and subsequently tested in vitro for phosphate solubilization and indole acetic acid (IAA) production. Pikovskaya's medium was used to estimate rhizomicrobial isolates to solubilize tricalcium phosphate [$Ca_3(PO_4)_2$]. Among all the investigated strains, 82 % of rhizospheric fungi showed phosphate solubilization activity; however, only 57.1 % of the rhizobacteria isolates showed phosphate solubilization ability. For IAA production, 64.7 % of the tested rhizofungi isolates were able to produce the phytohormone; however, only 47.62 % of the rhizobacteria isolates exhibited IAA production. Among all investigated species, Pseudomonas fluorescence and Azotobacter chroococcum showed the highest phosphate solubility demonstrating 885.4 and $863.4{\mu}gmL^{-1}$, respectively. Mucor sp. produced $42.3{\mu}gmL^{-1}$ of IAA in Czapek's tryptophan medium, and the highest fungal species to solubilize the inorganic phosphate ($237.5{\mu}gmL^{-1}$) was estimated by Penicillium sp. Rhizobacteria were more effective than rhizofungi in phosphate solubilization and IAA production. This study introduces some potent species in terms of phosphate solubilization and IAA production which could be likely to improve soils' quality and promote plant growth.
근권세균 균주인 Pseudomonas putida가 생성하는 Siderophores의 정화 및 화학적 특성분석
칼리드후세인 ( Khalid Abdallah Hussein ),이영돈 ( Young Don Lee ),주진호 ( Jinho Joo ) 한국환경농학회 2017 한국환경농학회 학술대회집 Vol.2017 No.-
Most of the microorganisms generate siderophores in iron deficient condition. Siderophores are low-molecular weight chelating agents produced by microorganisms and higher plants to encourage take-up of iron. It is reported that heavy metals in the milieu influence siderophores biosynthesis by microorganisms. In this study, we evaluated the effect of zinc ions, in the case of iron deficiency, on the siderophores generation by an array of rhizobacteria. Sixteen strains were specifically isolated from the rhizosphere of ginseng plant (Panax schinseng). The siderophores-producing rhizobacteria were investigated using chrome azurol S (CAS)-liquid media provided with various concentrations of ZnSO4 of (50-250 μg mL-1). The siderophore-producing strain Pseudomonas KNUK9 indicated high identity (99% using 16S-rRNA sequencing) to P. putida and showed potent antagonistic effect in vitro against the phytopathogenic fungal strain Aspergillus niger. The Sephadex LH-20 purified siderophore of Pseudomonas KNUK9 strain was chemically investigated using UV, FTIR, and MALDI-TOF-MS spectra. Results demonstrated that the pyoverdine is the iron-binding compound produced by Pseudomonas KNUK9 strain, and it`s greatly influenced by the presence of Zn<sup>2+</sup> ions in the medium. 100 μg mL<sup>-1</sup> Zn<sup>2+</sup> ions concentration improved the siderophores production in all siderophores-producing bacterial strains.