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( Dong Ju Park ),( Yong Suk Lee ),( Jie Chang ),( Shu Jun Fang ),( Yong Lark Choi ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.3
Paenibacillus xylanilyticus KJ-03 was isolated from soil samples obtained from a field with Amorphophallus konjac plants. A gene encoding xylanase was isolated from KJ-03 and cloned using a fosmid library. The xynA gene encodes xylanase; it consists of 1,035 bp and encodes 345 amino acids. The amino acid sequence deduced from the P. xylanilyticus KJ-03 xylanase showed 81% and 69% identities with those deduced from the P. polymyxa E681 and Paenibacillus sp. HPL-001 xylanases, respectively. The xynA gene comprises a single domain, consisting of a catalytic domain of the glycosyl hydrolase (GH) 10 family. The xynA gene was expressed in Escherichia coli BL21 (trxB), and the recombinant xylanase was purified by Niaffinity chromatography. The purified xylanase showed optimum activity with birchwood xylan as a substrate at 40oC and pH 7.4. Treatment with Mg2+ and Li+ showed a slight decrease in XynA activity; however, treatment with 5 mM Cu2+ completely inhibited its activity. The results of the thin layer chromatography analysis indicated that the major hydrolysis product was xylobiose and small amounts of xylose and xylotriose. XynA showed increased activity with oat spelt xylan and birchwood xylan, but showed only slight activity with locust bean gum.
Evaluation of Malignancy Risk of Ampullary Tumors Detected by Endoscopy Using 2-[18F]FDG PET/CT
Chuang Pei-Ju,Wang Hsiu-Po,Tien Yu-Wen,Chin Wei-Shan,Hsieh Min-Shu,Chen Chieh-Chang,Hong Tzu-Chan,Ko Chi-Lun,Wu Yen-Wen,Cheng Mei-Fang 대한영상의학회 2024 Korean Journal of Radiology Vol.25 No.3
Objective: We aimed to investigate whether 2-[18F]fluoro-2-deoxy-D-glucose positron emission tomography/computed tomography (2-[18F]FDG PET/CT) can aid in evaluating the risk of malignancy in ampullary tumors detected by endoscopy. Materials and Methods: This single-center retrospective cohort study analyzed 155 patients (79 male, 76 female; mean age, 65.7 ± 12.7 years) receiving 2-[18F]FDG PET/CT for endoscopy-detected ampullary tumors 5–87 days (median, 7 days) after the diagnostic endoscopy between June 2007 and December 2020. The final diagnosis was made based on histopathological findings. The PET imaging parameters were compared with clinical data and endoscopic features. A model to predict the risk of malignancy, based on PET, endoscopy, and clinical findings, was generated and validated using multivariable logistic regression analysis and an additional bootstrapping method. The final model was compared with standard endoscopy for the diagnosis of ampullary cancer using the DeLong test. Results: The mean tumor size was 17.1 ± 7.7 mm. Sixty-four (41.3%) tumors were benign, and 91 (58.7%) were malignant. Univariable analysis found that ampullary neoplasms with a blood-pool corrected peak standardized uptake value in earlyphase scan (SUVe) ≥ 1.7 were more likely to be malignant (odds ratio [OR], 16.06; 95% confidence interval [CI], 7.13–36.18; P < 0.001). Multivariable analysis identified the presence of jaundice (adjusted OR [aOR], 4.89; 95% CI, 1.80–13.33; P = 0.002), malignant traits in endoscopy (aOR, 6.80; 95% CI, 2.41–19.20; P < 0.001), SUVe ≥ 1.7 in PET (aOR, 5.43; 95% CI, 2.00–14.72; P < 0.001), and PET-detected nodal disease (aOR, 5.03; 95% CI, 1.16–21.86; P = 0.041) as independent predictors of malignancy. The model combining these four factors predicted ampullary cancers better than endoscopic diagnosis alone (area under the curve [AUC] and 95% CI: 0.925 [0.874–0.956] vs. 0.815 [0.732–0.873], P < 0.001). The model demonstrated an AUC of 0.921 (95% CI, 0.816–0.967) in candidates for endoscopic papillectomy. Conclusion: Adding 2-[18F]FDG PET/CT to endoscopy can improve the diagnosis of ampullary cancer and may help refine therapeutic decision-making, particularly when contemplating endoscopic papillectomy.
Effect of synthesis conditions on the particle size and morphology of YAG powder
Jie-Guang Song,Fang Wang,Ming-Han Xu,Yin-Yan Ju,Yang-Liang Li,Shi-Bin Li,Gang-Chang Ji 한양대학교 세라믹연구소 2012 Journal of Ceramic Processing Research Vol.13 No.2
Yttrium aluminum garnet (YAG) is currently a widely applied structural and functional material. To prepare highperformance YAG ceramics we prepared pure YAG powder, after calcining the precursor at 1100 ºC, by co-precipitation with ammonia as the precipitant and aluminum nitrate as well as yttrium nitrate as raw materials. The preparation conditions for the YAG precursor were a pH of 9, a titration rate of 10 ml/ minute and a reaction time of 60 minutes. The mean paricle size of the YAG powder was 11ìm. The mean particle size for the YAG powder increased with an increase in the pH and at a higher pH the YAG particles were more irregular in shape. The mean particle size and particle size distribution range of the YAG powder increased with an increase in the titration rate. For the YAG powder with a higher mean particle size, the particle size distribution range was wider but the size of most YAG particles was still small and the YAG particles were nearly spherical.
Effect of raw materials on the properties of ZrB2-YAG-Al2O3 multi-phase ceramics
Jie-Guang Song,Fang Wang,Da-Ming Du,Yin-Yan Ju,Shi-Bin Li,Gang-Chang Ji,Lian-Meng Zhang 한양대학교 세라믹연구소 2014 Journal of Ceramic Processing Research Vol.15 No.2
ZrB2 belongs to a class of ceramics defined ultra-high-temperature ceramics with extremely high melting temperatures, but ZrB2 ceramics is difficultly sintered and easily oxidized. To make ZrB2 ceramics possess the high relative density and the better oxidation resistance. The effects of raw materials on the properties of ZrB2 composite were investigated. YAG and Al2O3 help for the densification of ZrB2 ceramics. Fracture toughness of sintered ceramics with coated powder is higher than that of sintered ceramics with mixed powder. The mechanical property of ZrB2-YAG-Al2O3 materials is higher than that of ZrB2-YAG materials. Oxidation layer thickness of sintered ceramics with coated powder is thinner than that of sintered ceramics with mixed powder. These results show the sintered ZrB2 based multi-phase ceramics with coated microstructure help to increase the mechanical properties and oxidation resistance. The oxidation resistance of ZrB2-YAG-Al2O3 ceramics is better than that of ZrB2-YAG materials.
Effect of the sintering technology on the properties of fired brick from quartz sands
Jie-Guang Song,Fang Wang,Xiao-Bo Bai,Da-Ming Du,Yin-Yan Ju,Ming-Han Xu,Gang-Chang Ji 한양대학교 세라믹연구소 2011 Journal of Ceramic Processing Research Vol.12 No.4
River sands as low grade natural raw materials are used to prepare the fired brick to replace clay fired bricks, which aims to save land resources and use low grade natural resources to realize high cost performance. In this paper, the effect of sintering technology of bricks on their performance is discussed. The compressive strength of fired bricks is first increased and then decreased with an increase in the sintering temperature, the compressive strength of bricks prepared by the isostatic compaction shaping is higher than that of bricks prepared by the plastic shaping under the same conditions. The compressive strength of bricks reaches 28 MPa when sintered at 1150 oC by using a 20MPa isostatic compaction shaping method. The compressive strength of bricks is first increased and then decreased by prolonging the holding time, the compressive strength of bricks reaches 28.9MPa when sintered at 1150 oC for 2 h.
Wu Sing-Shan,Tseng Ching-Tzu,Yang Yu-Hung,Liu Yao-Chia,Chang Ju-Chun,Gyawali Purushottam,Li Yi-Hsuan,Yang Tzu-Hao,Tsai Yi-Fang,Tang Li-Cheng,Nai Yu-Shin 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.4
The fall armyworm, Spodoptera frugiperda, is an emerging invasive pest in Taiwan that feeds on a wide range of crops and causes serious damage. Herein, an entomopathogenic fungal library (EFLib) was constructed to identify potential microbes to control FAW. Twenty-eight indigenous entomopathogenic fungi (EPF) were iso lated and investigated for their potential pathogenicity, with Metarhizium pinghaense (Mp-NCHU-124) and Beauveria bassiana (Bb-NCHU-157) exerting dose-dependent effects on the 4th instar FAW larvae. The non-ionic surfactant Silwet L-77 rapidly killed FAW larvae after spraying at a concentration of 300 mg/kg and the toxic effect of Silwet L-77 on FAW larvae was dose-dependent. When the EPF isolates (10 6 conidia/mL) were applied to FAW larvae in combination with the non-ionic surfactant Silwet L-77 (30–90 mg/kg), the mortality rate dramatically increased and the LT 50 reduced, with increased fungal mycosis (Mp-NCHU-124: 38% to 72% and Bb-NCHU-157: 20 to 62%), indicating the high compatibility of EPF with the non-ionic surfactant. Thus, the Silwet L-77+EPF combined formulation has potential for practical field application for FAW pest control and sustainable agriculture in the future.