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Lee, Sang Keum,Lee, Hyungjae Korean Society of Food Science and Technology 2016 Food Science and Biotechnology Vol.25 No.5
Forty solvent fractions and 387 bacterial isolates of seven varieties of Korean domestic honey and manuka honey from New Zealand were screened for antimicrobial activity. The minimum inhibitory concentrations and minimum bactericidal concentrations of the honey fractions were determined; only Bacillus cereus ATCC 14579, ATCC 11778, and F4552 were inhibited by 11, 1, and 16, respectively, out of the 40 honey fractions. The bacterial isolates showed the highest incidence (30.2%) of antimicrobial activity against Listeria monocytogenes ATCC 15313. The growth of at least one of the five foodborne pathogens tested was inhibited by 109 of the 327 isolates (33.3%) from seven types of Korean domestic honey. The percentage of such isolates of manuka honey was significantly higher (76.7%). Solvent fractionation of honey could contribute to the detection of antimicrobial activity of the nonsugar compounds in honey. Moreover, the bacterial isolates from Korean domestic honey may be good sources for the natural antimicrobials used in the food industry and other related industries.
Lee, HyungJae,Hong, Wooyoung,Jeon, Seunghyun,Choi, Yongdoo,Cho, Youngnam American Chemical Society 2015 Langmuir Vol.31 No.14
<P>An electroresponsive drug release system based on polypyrrole (Ppy) nanowires was developed to induce the local delivery of anticancer drug, doxorubicin (DOX), according to the applied electric field. DOX-conjugated Ppy nanowire (NW) (DOX/Ppy NW) array was initially prepared by electrochemical deposition of a mixture of pyrrole monomers and biotin as dopants in the anodic alumina oxide membrane as a sacrificial template. Morphological observation by scanning electron microscopy revealed free-standing and 3D nanotopographical features with large surface area and high density. In addition, we investigated the antitumor efficacy of DOX released from DOX/Ppy NW array in response to the external electric field using two kinds of cancer cell lines, human oral squamous carcinoma cells (KB cells) and human breast cancer cells (MCF7 cells). Meanwhile, strong photothermal effect as a result of a near-infrared absorbing ability of Ppy synergistically maximizes the chemotherapeutic efficacy. Our results suggested that the proposed multifunctional Ppy platform possessing several beneficial features is very promising for many therapeutic applications including cancer.</P>
Lee, HyungJae,Choi, Mihye,Jo, Minkyung,Park, Eun Young,Hwang, Sang-Hyun,Cho, Youngnam Elsevier 2020 Gynecologic oncology Vol.156 No.3
<P><B>Abstract</B></P> <P><B>Objective</B></P> <P>To evaluate whether HPV DNA in urine has potential advantages as an alternative biomarker for HPV-based cervical cancer screening.</P> <P><B>Methods</B></P> <P>Among patients with Cobas HPV test results, a total of 67 HPV-positive (n = 42) and -negative (n = 25) women who agreed to participate in this study were willing to provide paired cervical and urine samples, and we observed concordance between sample types from each patient in identifying HPV genotypes using the nanowire assay.</P> <P><B>Results</B></P> <P>We detected high-risk strains of HPV DNA in unprocessed urine specimens using polyethyleneimine-conjugated nanowires (PEI-NWs). Concordance for high-risk HPV (hrHPV) between paired urine and cervical samples was 90.4% (κ = 0.90; 95% CI: 0.80–100.00). The virological sensitivity and specificity for detection of HPV DNA from a small urine sample (200 μL) were 81.3% (κ = 0.83; 95% CI: 62.1–100.0) and 98.0% (κ = 0.83; 95% CI: 94.2–100.0) for HPV16 group, 100.0% (κ = 0.65; 95% CI: 100.0–100.0) and 95.3% (κ = 0.65; 95% CI: 90.1–100.0) for HPV18 group, and 96.4% (κ = 0.97; 95% CI: 89.6–100.0) and 100.0% (κ = 0.97; 95% CI: 100.0–100.0) for other hrHPV group, respectively.</P> <P><B>Conclusions</B></P> <P>The nanowire assay demonstrated excellent ability to identify HPV DNA from urine specimens. We observed an excellent agreement in the detection of high-risk HPV between paired urine and cervical samples, even with small urine sample volume.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Nanowire assay can detect HPV DNA in urine with high sensitivity and specificity. </LI> <LI> Non-invasive, urine-based HPV screening can increase cervical cancer effectiveness. </LI> <LI> HPV type-specific concordance rate between urine and cervical samples was over 90%. </LI> </UL> </P>
Lee, HyungJae,Hwang, Na Rae,Hwang, Sang-Hyun,Cho, Youngnam Elsevier 2016 Biosensors & bioelectronics Vol.86 No.-
<P><B>Abstract</B></P> <P>Detecting human papillomavirus (HPV) is central in diagnosing and monitoring HPV-related disease. However, limited sensitivity and the wide variability of the HPV genome pose challenges in the identification of HPV genes, particularly high-risk types. This study reports the development of polyethyleneimine-conjugated magnetic nanowires (PEI-MNWs) and their use in the isolation, identification, and analysis of multiple genotypes of HPV DNA from cervical cancer specimens. The nanowires are electrochemically doped with a high density of magnetic nanoparticles and biotin moieties during potentiostatic deposition, thereby allowing conjugating cationic branched polymers to direct the attachment of negatively charged DNA molecules with strong magnetic response. For proof of concept, the rapid and ultrasensitive isolation of HPV DNA is performed at concentrations as low as 10pg/mL with an efficiency of >95%. For clinical optimization, the analytical and clinical sensitivity of PEI-MNWs is compared with that of the Roche Cobas 4800 HPV Test and demonstrates excellent correlation for multiple HPV DNA genotypes with superior threshold cycle values. The high sensitivity, specificity, and good reproducibility of PEI-MNWs are particularly well suited for the recovery of DNA and provide significant and clinically meaningful evidence for the early detection and treatment of HPV-associated cancers.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The magnetic nanowires were electrochemically developed via potentiostatic deposition. </LI> <LI> Nanowires enhance the attachment of polymer chains that ultimately improve DNA recovery. </LI> <LI> Nanowires demonstrated the isolation and analysis of multiple genotypes of HPV DNA. </LI> <LI> HPV DNA detection and genotyping was confirmed for analytical and clinical validation. </LI> </UL> </P>
제품 설계 정보와 영상 데이터의 병합을 위한 에지 기반 라벨링에 의한 영상 분할
이형재 ( Hyungjae Lee ),김용일 ( Yongil Kim ),양형정 ( Hyungjeong Yang ) 한국정보처리학회 2005 한국정보처리학회 학술대회논문집 Vol.12 No.2
본 논문에서는 협동적 제품 개발 환경에서 제품 설계 데이터와 제품 내의 객체 정보를 매칭하고 영상 기반에서 공학 데이터를 검색하기 위한 목적으로 영상 내의 객체의 각 영역을 분할 하고자 한다. 제품 설계시 생성 과정에서 CAD 툴 등으로부터 생성되는 영상은 객체 화소값의 차이가 적고 생산환경에 맞게 실시간으로 정보를 제공 할 수 있어야 한다. 위와 같은 두 가지 사항을 해결하기 위해, 전처리 과정이 없이 객체 내의 각 부분 정보를 알 수 있는 에지 기반 라벨링(Edge_Based Labeling) 기법을 제안한다.
북 핵 위협 대응을 위한 지휘통제체계 발전방안 : 인공지능 기술을 적용한 지능화 항공우주작전본부 구축
이형재(Hyungjae Lee) 육군사관학교 화랑대연구소 2021 한국군사학논집 Vol.77 No.3
If we have a command and control system that guarantees a more swift and accurate decision-making than the enemy, we can exert greater power with only our existing forces, and ultimately increase our deterrence against the enemy. Therefore, this study asserts that in order to maximize the efficacy of Korea"s nuclear and WMD response system, it is necessary to enhance the command and control capabilities along with force reinforcement. The quality and speed of decision-making can be improved by applying AI technology to the Air & Space Operations Center, which is the command and control system to be used by the operations center of the nuclear and WMD response system. By applying AI technology to AOC, it will be possible to simultaneously respond to North Korea"s conventional threats as well as nuclear and missile threats.
알칼리성 단백질 분해효소를 생산하는 토양 분리 호알칼리성 균주의 동정
이형재 ( Hyungjae Lee ),유지승 ( Ji Seung Yoo ),박영서 ( Young Seo Park ),배동훈 ( Dong Hoon Bai ) 한국산업식품공학회 2015 산업 식품공학 Vol.19 No.4
An alkalophilic microorganism, strain DK1122 producing an alkaline protease was identified. DK1122 was isolated from soil collected in central Korea. The strain DK1122 was Gram-positive, 0.7×2-4 μm in size, and its colony was yellowish white, The strain DK1122 was found to be spore-forming, catalase positive, oxidase positive, caseinolytic, and reduce nitrate to nitrite. The protease was produced aerobically on Horikoshi I agar medium (pH 9.0) with 1% (w/v) skim milk at 40°C for 24 h. Through 16S rRNA gene partial sequencing, the strain DK1122 had the 99.7% sequence similarity to 16S rRNA gene sequence of Bacillus pseudofirmus. Based on the biochemical and physiological properties as well as phylogenetic analysis, the isolated strain was named as Bacillus sp. DK1122. It is expected that Bacillus sp. DK1122 may be a promising candidate for a proudcer of an alkaline protease applicable to the food and detergent industries.