U-phosphate biomineralization induced by Bacillus sp. dw-2 in the presence of organic acids

Hong Tu,Guoyuan Yuan,Changsong Zhao,Jun Liu,Feize Li,Jijun Yang,Jiali Liao,Yuanyou Yang,Ning Liu 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.5

In this paper, we systematically investigated the influence of some selected ligands on the U-phosphateprecipitation induced by soil bacteria. These organics are widely ranging from acetate, lactate, salicylateand citrate to oxalate. The results revealed that uranium could be biomineralized on bacteria asUO2HPO4$4H2O or (UO2)3(PO4)2$4H2O. The influence of organic ligands on the biomineralization hadclear-cut correlations with its complexation abilities to uranyl. It was clearly found that the U-phosphatebiomineralization was affected noticeably by the strong ligands (oxalate and citrate). Further studydiscovered that when the organic ligands were uncompetitive with biotic PO4 3 for uranyl, the transformationof uranyl species from b-UO2(OH)2 colloidal particles to free UO2 2 þ-ligands ions could facilitatethe U-phosphate biomineralization. However, when the organic ligands competed with biotic PO4 3 foruranyl, the U-phosphate biomineralization were inhibited. Our results highlight the importance ofcomplex interactions of strong organic ligands with uranyl during the bacterial precipitation of UePcompounds and thus for the mobilization and immobilization of radio-nuclides in the nature.

Treatment of wastewater containing linear alkylbenzene sulfonate by bacterial-microalgal biological turntable

Renjie Tu,Wenbiao Jin,Song-fang Han,Binbin Ding,Shu-hong Gao,Xu Zhou,Shao-feng Li,Xiaochi Feng,Qing Wang,Qinhui Yang,Yu Yuwen 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.5

Linear alkylbenzene sulfonate (LAS), which is widely used as detergent, is a common toxic pollutant in wastewater. Generally, biodegradation process is applied to remove LAS. However, the efficiency of traditional wastewater treatment cannot meet the growing demand. In this study, an improved biological turntable with a symbiotic system of bacteria and microalgae was primarily used to enhance the biodegradation efficiency of LAS from wastewater. The symbiotic system of bacteria and microalgae was mainly composed of Scenedesmus dimorphus and three LAS-degrading bacteria Plesiomonas sp. (L3, L7) and Pseudomonas sp. (H6). The average removal rate of LAS was up to 94.6%. The LAS concentration of the effluent of the system decreased by 81.7% after the bacterial-microalgae inoculation (the inoculation temperature was 25 oC; microalgae were inoculated at a concentration of 10% only at the start of the system; bacteria were continuously inoculated at 1‰ concentration). After bacterial-microalgae inoculation, the average effluent concentration of CODCr in the tertiary reaction tank was 24.3mg/L, the average membrane effluent concentration was 15.8mg/L, and the average removal rate was 90.5%. Compared with the control group without inoculation, the concentration of CODCr in the tertiary reaction tank and membrane effluent decreased by 55.7% and 46.4%. The denaturing gradient electrophoresis (DGGE) pattern analysis of the systemic flora showed that there were two dominant species of high LAS degrading bacteria. They were identified to belong to Plesiomonas sp. and Pseudomonas sp., respectively.

Survivin Gene Functions and Relationships between Expression and Prognosis in Patients with Nasopharyngeal Carcinoma

Cai, Jun-Hong,Fu, Sheng-Miao,Tu, Zhi-Hua,Deng, Li-Qun,Liang, Zhu,Chen, Xin-Ping,Gong, Xuan-Ju,Wan, Li-Hua Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6

This study aimed to investigate the relationship between prognosis and protein and mRNA expression of an apoptotic inhibitor gene, survivin, in patients with nasopharyngeal carcinoma. Furthermore, functions of the survivin gene in the CNE2 nasopharyngeal carcinoma cell line were assessed. Immunohistochemistry and in situ hybridization were used in detecting the survivin protein and mRNA in 44 nasopharyngeal carcinoma specimens, and 30 chronic nasopharyngitis samples as controls. Survivin gene expression in CNE2 cell line was suppressed with an shRNA (short hairpin RNA). The positive ratios of expression for survivin protein and mRNA in nasopharyngeal carcinoma were 79.5% and 75.0% respectively, obviously higher than in the control group (p<0.01), and there is very good consistency between the two methods. The mean survival time of patients with higher survivin protein or mRNA expression was shorter than in patients with lower levelsv(p<0.01). Proliferation of the CNE2 cell line was distinctly inhibited by the shRNA. The results indicate that overexpression of the survivin gene plays an important role in onset and development of nasopharyngeal carcinoma, and it may be helpful for prognostic appraisal.

Effects of habitat fragmentation on the functional diversity of insects in Thousand Island Lake, China

Tian‐Hao Tu,Ji-Rui WANG,Jian-Qiang GU,Tian-Qi LI,Kun LV,Guo-Xin ZHOU,Zhi-Hong XU 한국곤충학회 2019 Entomological Research Vol.49 No.2

Due to habitat fragmentation, the loss of species diversity has been extensively studied. On the contrary, the effects of habitat fragmentation on functional diversity is still poorly understood. In the Thousand Island Lake, we conducted studies of insect functional diversity on a set of 29 isolated islands. We used 10 functional diversity indices from three aspects (functional richness, functional evenness and functional divergence) to respectively describe functional diversity of insects on sample islands. We found the following results: (i) The functional indices selected could reflect the functional diversity of sample islands and it is further proved that in general, three components of functional diversity were independent of each other; (ii) Sample islands could be divided into two categories, island JSD and the remaining islands; (iii) Functional richness increased with island area and shape index, but had no significant correlation with isolation. Likewise, both functional evenness and functional divergence had no significant correlation with island attributes. The conclusion to emphasize from our research is that: (i) habitat fragmentation reduced the biological functional diversity to some extent, further demonstrating the importance of habitat continuity in biodiversity protection; and (ii) for functional diversity protection of insects in a fragmented landscape, an island which has high approximate shape index values of at least hundred hectare magnitude order has a critical promoting effect.

The Efficacy of Aspirin in Preventing the Recurrence of Colorectal Adenoma: a Renewed Meta-Analysis of Randomized Trials

Zhao, Tai-Yun,Tu, Jing,Wang, Yin,Cheng, Da-Wei,Gao, Xian-Kui,Luo, Hao,Yan, Bi-Chun,Xu, Xiao-Li,Zhang, Hong-Ling,Lu, Xing-Jun,Wang, Yao-Jun Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.5

Background: Through search the possible randomized control trials, we make a renewed meta-analysis in order to assess the impact of aspirin in preventing the recurrence of colorectal adenoma. Materials and Methods: The Medicine/PubMed, Embase, Cochrane Central Register of Controlled Trials (CENTRAL), Chinese biomedical literature service system (SinoMed) databases were searched for the related randomized controlled trials until to the April 2016. Three different authors respectively evaluated the quality of studies and extracted data, and we used the STATA software to analyze, investigate heterogeneity between the data, using the fixed-effects model to calculate and merge data. Results: 7 papers were included the renewed meta-analysis, among these studies, two pairs were identified as representing the same study population, with the only difference being the duration of follow-up. Thus there were only five papers included our meta-analysis, and one Chinese paper were also included the work. Results were categorized by the length of follow-up, different kinds of people, varied dose of oral aspirin. The relative of adenoma in patients taking aspirin vs placebo were 0.73 (95% CI 0.55-0.98, P=0.039) with 1 year follow up; 0.84 (95% CI 0.72-0.98, P=0.484) with greater than 1 year follow up; for the advanced adenoma, the RR 0.68 (95% CI 0.49-0.94, P=0.582),for one year; RR=0.75 (95% CI 0.52-1.07, P=0.552) for greater one year. Furthermore the white population could divided into two subgroups according to the different length of follow-up time. When the length of follow-up time less than 3-year, The RR of two subgroups respective were RR=0.86 (95% CI 0.76-0.98, P=0.332), $I^2=0%$, RR=0.68 (95% CI 0.47-0.98, P=0.552), $I^2=64.6%$, But with the extension of follow-up time greater than 2-year, with the white, oral aspirin without considering dose had no efficacy on preventing the recurrence of any adenoma, the RR was 0.86 (95% CI 0.71-1.05, P=0.302), $I^2=16.4%$. Conclusions: This meta-analysis indicated that oral aspirin is associated with a remarkable decrease in the recurrence of any adenoma and advanced adenomas in patients follow-up for 1 year without concerning the dose of aspirin, but with the extension of follow-up time for greater than 1 year, oral aspirin can be effective on preventing the recurrence of any adenoma, but for the advanced adenoma, the result indicated that oral aspirin had no efficacy, According to the inclusion of ethnic groups, we also divided relevant papers into two subgroups as the yellow and white group. Then the follow-up time was less than 3 years, oral aspirin without considering the dose, had an significant efficacy on preventing the recurrence of any adenoma. But with the follow-up greater than 2 years, oral aspirin had no effect in the white.

The Classic Security Application in M2M: the Authentication Scheme of Mobile Payment

( Liang Hu ),( Ling Chi ),( Hong-tu Li ),( Wei Yuan ),( Yu Yu Sun ),( Jian-feng Chu ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.1

As one of the four basic technologies of IOT (Internet of Things), M2M technology whose advance could influence on the technology of Internet of Things has a rapid development. Mobile Payment is one of the most widespread applications in M2M. Due to applying wireless network in Mobile Payment, the security issues based on wireless network have to be solved. The technologies applied in solutions generally include two sorts, encryption mechanism and authentication mechanism, the focus in this paper is the authentication mechanism of Mobile Payment. In this paper, we consider that there are four vital things in the authentication mechanism of Mobile Payment: two-way authentication, re-authentication, roaming authentication and inside authentication. Two-way authentication is to make the mobile device and the center system trust each other, and two-way authentication is the foundation of the other three. Re-authentication is to re-establish the active communication after the mobile subscriber changes his point of attachment to the network. Inside authentication is to prevent the attacker from obtaining the privacy via attacking the mobile device if the attacker captures the mobile device. Roaming authentication is to prove the mobile subscriber`s legitimate identity to the foreign agency when he roams into a foreign place, and roaming authentication can be regarded as the integration of the above three. After making a simulation of our proposed authentication mechanism and analyzing the existed schemes, we summarize that the authentication mechanism based on the mentioned above in this paper and the encryption mechanism establish the integrate security framework of Mobile Payment together. This makes the parties of Mobile Payment apply the services which Mobile Payment provides credibly.

Applicability Analysis of Microseismic Technology in Tunnel Water Inrush Monitoring

Yan-hao Chen,Shuai Cheng,Li-Ping Li,Jun-yan Yang,Hong-liang Liu,Wen-feng Tu 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.7

Water inrush disaster is one of the most serious geological disasters in tunnel construction. There are still some problems in the existing researches, such as the activity information of surrounding rock is difficult to obtain, the mechanism of water inrush disaster is unclear, the correlation between water inrush disaster and microseismic (MS) information is missing, which have caused the water inrush disaster to be passively controlled. Combining with the practical experience of tunnel engineering, the application problems of MS technology in tunnel engineering is summarized, which provides ideas for monitoring of surrounding rock activity. The formation mechanism of water inrush disaster caused by rock mass fracturing and filling medium instability has been revealed. The MS characteristics of the water inrush channel in different water inrush disasters have also been revealed. The MS response characteristics of water inrush and rockburst disasters are compared, which provides guidance for the improvement of MS monitoring technology and the analysis of effective MS information. The compression failure test of limestone under saturated and natural conditions is carried out. The results show that the presence of water weakens the ability of rock to store energy. The acoustic emission (AE) phenomenon is weaker in the process of saturated limestone failure, which increases the difficulty of AE monitoring. The number of AE events of natural limestone and saturated limestone shows a slow increase to a sudden increase in the process stress loading, which can be regarded as an important precursor of rock failure.

The mutation of Scenedesmus obliquus grown in municipal wastewater by laser combined with ultraviolet

Xu Zhou,Wenbiao Jin,Song-fang Han,Xiaoye Li,Shu-Hong Gao,Chuan Chen,Guo-jun Xie,Renjie Tu,Qing Wang,Qilin Wang 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.6

Mutagenetic breeding is an efficient technique for the enhancement of lipid productivity from microalgae. In this study, oil-rich microalga Scenedesmus obliquus were treated by Laser-UV composite mutagenesis. Among the 35 mutant strains, X5 was primely screened. Afterwards, a twice UV mutagenizing was operated on X5, and the optimal mutant strain X5-H13 was obtained. The growth rate, dry weight, lipid yield and lipid content of X5-H13 were 0.698× 107 cells/mL·d, 0.99 g/L, 0.49 g/L and 48.8% while cultivated in municipal wastewater, respectively, which were increased by 45%, 58%, 109% and 32% than the original strain. The results of the subculture of repeated mutant showed that the biomass and lipid content of strain X5-H13 were up to 0.99 g/L and 48.8%. The growth of each generation was stable. Furthermore, the random amplified polymorphic DNA analysis indicated that the mutant strain X5-H13 was different from the starting strain, with their genetic similarity coefficient value of 0.815.