A New Insight on Phylogenetic Relationships of Elateridae (Coleoptera) Inferred from 12 Multiple Loci

Taeman Han,Haechul Park,Youngbo Lee,In Gyun Park,Jongchel Jeong,Seunghwan Lee 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

The previous studies for phylogenetic relationships within Elateridae were carried out, but not constructed a reliable evolutionary hypothesis. This study attempted to establish a robust evolutionary hypothesis, focusing on major subfamilies of the family Elateridae sensu stricto, using extensively selected 12 genetic markers, COI, 16S rRNA, 18S rRNA, 28S rRNA and 8 nuclear genes. As the results, phylogenetic analyses for 12 multiple genes constructed robust phylogeny with almost very strongly supported nodal values (>90%) and represented that the previously questioned systematic positions of nine subfamilies are fully resolved, excepting the basal lineage split. Especially, three subfamilies, which were recently reduced into tribal rank, Hypnoidinae, Oxynopterinae, and Denticollinae are monophyletic, respectively, and it is supported the traditional taxonomic schemes that had been treated these three taxa in subfamily rank. Whereas, Elaterinae, Hiopinae, and Melanotinae are clustered to a monophyletic group. Two tribes, Denticollini and Ctenicerini in Denticollinae are paraphyletic, respectively and it needs to reclassify their systematic positions. This study recovering of relationships between subfamilies using 12 gene loci resulted that their phylogenetic relationships are sufficiently and successfully resolved with strong supported nodes and provided more possible interpretations from subfamily to tribal levels than the previous studies.

A simplified diet for rearing Agrius convovuli (Lepidoptera: Sphingidae)

Seonghyun Kim,Haechul Park,Ingyun Park 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.04

Artificial insect diets are a crucial factor of many insect rearing systems for research purposes. Three different diets were prepared by formulating variations of the standard diet using various proportions of dried potato powder and wheat germ. The larvae were reared on these diets under long day conditions (25℃, 14L). Fitness parameters including larval development, immature survival, pupal weight, pupation, adult emergence were measured to evaluate the performance of Agrius convovuli. The percentage of pupation varied considerably, with significant differences among diets, on which a maximum pupation percentage of 95.0% was observed. The larvae reared from hatching on diet A completed larval development in 29.1d, and adult emergence occurred on day 17.6d. An analysis of variance showed significant differences among the three diets in the mean pupal weight. These results indicated that A. convovuli adapted well to the artificial diet A and successive rearing conditions. The diet A could serve as viable alternative to natural host plants for consecutive rearing of the insect. The successful development of the diet and rearing technique provides a very useful tool for refining hawk moth.

The First Record of Conilepia nigricosta (Leech) in Korea with Comparison of Morphologically Similar Species, Lithosia quadra (Linnaeus) (Lepidoptera, Erebidae, Arctiinae) Inferred from Morphological and Molecular Approaches

Ga-Eun Lee,Taeman Han,Haechul Park,In Gyun Park,Joon-Bum Kim 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

We report Conilepia nigricosta (Leech, 1988) in Korea for the first time. The female of C. nigricosta is nearly identical to the female of Lithosia quadra, and it has caused confusion in the generic classification. Therefore, we reexamined their morphology and also performed to analyze DNA barcode region compared with species of other related genera. As the results, the significant diagnoses between the two species were reconfirmed and provided in the adult appearances, wing venations, and genital structures in both sexes. The DNA barcoding results also showed a large genetic distance ranging from 6.21% to 7.16% between the two species. In phylogenetic relationship for these two species as well as 28 related species of Lithosiina, C. nigricosta was clustered as a sister to L. quadra and they formed a strong monophyletic group. It supports a possibility that these two genera may be a single genus, but more detailed molecular analysis using additional genetic markers and samples is needed to resolve reliable phylogenetic relationships between the taxa examined in this study.

Genetic diversity of Insect farm populations of Protaetia brevitarsis seulensis (Kolbe, 1886) (Coleoptera, Scarabaeidae) in South Korea inferred from COI gene sequences

Taeman Han(Taeman Han),Seung-Chul Park(Seung-Chul Park),Haechul Park(Haechul Park) 국립공원연구원 2022 국립공원연구지 Vol.13 No.1

A Korean indigenous species, Protaetia brevitarsis seulensis (PBS), is popularly reared at many insect farms as valuable industrial insect resources in South Korea. However, there is no comprehensive study on the genetic diversity within the Korean PBS population as well as between morphologically closed species. Employing the rapidly evolved genetic markers such as mitochondrial DNA is important in the detection of haplotype diversity and rapid species identification of larval stage and/or its processed goods. We here examined how many genetically different haplotypes distribute in the reared PBS collected from 14 insect farms and the interspecific genetic difference between congeners using mitochondrial COI gene sequences (DNA barcoding region). As a results, the PBS was clearly separated from two related species with large genetic distances (7.98%~8.86%). In the PBS, 57 haplotypes were detected from 223 specimens with low intraspecific genetic distance (~1.3%). Among them, the most dominant haplotypes are subsequently detected in H4 (25.6%), H38 (15.7%) and H26 (10.3%). From the haplotype network and distribution analyses, we consider that many haplotypes have been mixed by artificial collections from various local places and exchanged between insect farms within South Korea. We expect that this data will be helpful in rapid species identification for its processed goods, and understanding the exact population diversity and detection of the original habitats for each haplotype of the reared PBS throughout comparing with natural populations in the further study.

The history and value of pest's Korean names led by the Japanese Government General of Korea

Haechul Park,Gwan-Seok Lee 한국응용곤충학회 2021 한국응용곤충학회 학술대회논문집 Vol.2021 No.04

Taxonomic Review of Denticollinae (Coleoptera, Elateridae) in Korea and Molecular Phylogeny of Elateridae

Taeman Han,Haechul Park,Seunghwan Lee 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

For reviewing Korean species of Denticollinae and reconstructing phylogenetic relationships between subfamilies in Elateridae, three kinds of studies were conducted: 1) taxonomic review based on morphology; 2) DNA barcode for Denticollinae; 3) molecular phylogenetic study of Elateridae using 12 genetic markers. In taxonomic study, a total of 54 species were reviewed including 13 new species and then unrecorded species. In DNA barcoding, six cryptic and/or pseudocryptic species were confirmed by their sympatric or allopatric distribution. We propose a conservative threshold of 3.6% for convenient molecular operational taxonomic unit identification in Denticollinae. From this study, we confirmed that COI sequence is useful for reassessing species diversity for polymorphic and polytypic species occurring in sympatric and allopatric distributions. In molecular phylogenetic study, the phylogenetic inference constructed robust phylogeny with almost very strongly supported nodal values(>90%) and showed that Hypnoidinae, Oxynopterinae and Denticollinae are monophyletic; Elaterinae, Hemiopinae and Melanotinae are a monophyletic group; and two tribes in Denticollinae are paraphyletic, it is needed to reclassify their systematic accounts.

Taxonomic Review of the Genus Capsus Fabricius (Hemiptera: Heteroptera: Miridae: Mirinae) from the Korean Peninsula

Junggon Kim,Haechul Park,Sunghoon Jung 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

The genus Capsus Fabricius (Hemiptera: Heteroptera: Miridae: Mirinae) from the Korean Peninsula is reviewed. Biological information such as host plants and distributions, and important morphological characters for each species are presented. Photographs of the Korean specimens and illustrations of male genitalia, and diagnoses based on main characters with a key to the Palaearctic species are provided.

A Taxonomic Review of the Subfamily Dendrometrinae (Coleoptera, Elateridae) in Korea

Taeman HAN,Haechul PARK,Yongbo LEE,Seunghwan LEE 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

The subfamily Dendrometrinae is a large group in Elateridae with about 2000 species world wide. However, this subfamily were not taxonomically reviewed in the Korean fauna. As the result of taxonomic review, 40 species are confirmed from Korea, with the description of two new genera, 11 new species, and one new subspecies. A total of 11 species are also recognized for the first time from Korea.

A Taxonomic Review of the genus Scutellathous Kishii, 1955 and a New Genus, Subscutellathous (Coleoptera, Elateridae) from Korea

Taeman Han,Haechul Park,Jongchel Jeong,Seungwhan Lee 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

This taxonomic study on the genus Scutellathous is only recognized a species. S. porrecticollis (Lewis, 1894) in Korea. The previous record of Athous (=Medakathous) jactatus (Lewis, 1984) is revealed as the misientification of S. porrecticollis. We also describe and illustrate Subscutellathous koreanicus gen. and sp. nov. from Korea. This new genus si compared to the most closed genus Scutellathous by the basis of diagnostic characters in generic level.

Analysis of psychological effects expected from educational pet insect rearing

( Soyun Kim ),( Haechul Park ),( Ingyun Park ),( Kwanho Park ),( Namjung Kim ),( Seonghyun Kim ) 한국잠사학회 2015 International Journal of Industrial Entomology Vol.31 No.1

In an effort to obtain foundational data required to evaluate the therapeutic effect of educational insect rearing, this study surveyed current educational pet insect rearing practices in Korea and examined the expected psychological effects from participating in the activity. Data were collected from those who visited the special insect exhibition in May 2013 using a self-reporting survey. The results indicated that 48.3% of the responders had experienced insect rearing, and that rhino and stag beetles were the most commonly reared insects. Most of the respondents (83.1%) reported they were not currently rearing any insects, which suggested that insect rearing tends to be a one-time experience. Expected psychological effects could be divided into emotional (56.3%) and cognitive (41.8%) effects, and these varied by age and gender. The differences in expected psychological effects were particularly clear between adults and adolescents, the latter of whom were the main participants in the activity. It is therefore necessary to develop standardized manuals and care programs for more diverse insect species with which to help keep up consumer interest in educational insect rearing. These efforts will help champion the psychotherapeutic effects of educational pet insect rearing and enhance its role as a learning aid.