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RISS 인기검색어
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- 저자 : ( Deepmala Shrestha ) (검색결과 3 건)
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Shrestha, Deepmala,Choi, Daeun,Song, Kiwon Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.5
The actin cytoskeleton plays a key role in the entry of mitosis as well as in cytokinesis. In a previous study, we showed that actin disruption delays mitotic entry at G2/M by sustained activation of extracellular signal-related kinase 1/2 (ERK1/2) in primary cells but not in transformed cancer cell lines. Here, we examined the mechanism of cell cycle delay at G2/M by actin dysfunction in IMR-90 normal human fibroblasts. We observed that de-polymerization of actin with cytochalasin D (CD) constitutively activated ribosomal S6 kinase (RSK) and induced inhibitory phosphorylation of Cdc2 (Tyr 15) in IMR-90 cells. In the presence of an actin defect in IMR-90 cells, activating phosphorylation of Wee1 kinase (Ser 642) and inhibitory phosphorylation of Cdc25C (Ser 216) was also maintained. However, when kinase-dead RSK (DN-RSK) was overexpressed, we observed sustained activation of ERK1/2, but no delay in the G2/M transition, demonstrating that RSK functions downstream of ERK in cell cycle delay by actin dysfunction. In DN-RSK overexpressing IMR-90 cells treated with CD, phosphorylation of Cdc25C (Ser 216) was blocked and phosphorylation of Cdc2 (Tyr 15) was decreased, but the phosphorylation of Wee1 (Ser 642) was maintained, demonstrating that RSK directly controls phosphorylation of Cdc25C (Ser 216), but not the activity of Wee1. These results strongly suggest that actin dysfunction in primary cells activates ERK1/2 to inhibit Cdc2, delaying the cell cycle at G2/M by activating downstream RSK, which phosphorylates and blocks Cdc25C, and by directly activating Wee1.
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Deepmala Shrestha,최다은,송기원 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.5
The actin cytoskeleton plays a key role in the entry of mitosis as well as in cytokinesis. In a previous study, we showed that actin disruption delays mitotic entry at G2/M by sustained activation of extracellular signal-related kinase 1/2 (ERK1/2) in primary cells but not in transformed cancer cell lines. Here, we examined the mechanism of cell cycle delay at G2/M by actin dysfunction in IMR-90 normal human fibroblasts. We ob-served that de-polymerization of actin with cytochalasin D (CD) constitutively activated ribosomal S6 kinase (RSK) and induced inhibitory phosphorylation of Cdc2 (Tyr 15) in IMR-90 cells. In the presence of an actin defect in IMR-90 cells, activating phosphorylation of Wee1 kinase (Ser 642) and inhibitory phosphorylation of Cdc25C (Ser 216) was also maintained. However, when kinase-dead RSK (DN-RSK) was overexpressed, we observed sustained activation of ERK1/2, but no delay in the G2/M transition, demonstrating that RSK functions downstream of ERK in cell cycle delay by actin dysfunction. In DN-RSK overexpressing IMR-90 cells treated with CD, phosphorylation of Cdc25C (Ser 216) was blocked and phosphorylation of Cdc2 (Tyr 15) was decreased, but the phosphorylation of Wee1 (Ser 642) was maintained, demonstrating that RSK directly controls phosphorylation of Cdc25C (Ser 216), but not the activity of Wee1. These results strongly suggest that actin dysfunction in primary cells activates ERK1/2 to inhibit Cdc2, delaying the cell cycle at G2/M by activating downstream RSK, which phosphorylates and blocks Cdc25C, and by directly activating Wee1.
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Study and Evaluation of Tourism Websites based on User Perspective
( Deepanjal Shrestha ),( Tan Wenan ),( Neesha Rajkarnikar ),( Deepmala Shrestha ),( Seung Ryul Jeong ) 한국인터넷정보학회 2021 인터넷정보학회논문지 Vol.22 No.4
A well-designed website is mandatory for good marketing and proper tourism business. This research considers Nepal as a domain of study and specifically explores welcomenepal.com, the official tourism portal as a reference for the study. The work is based on the study of the existing literature, user-survey, and technical testing of the website using open-source testing tools to identify user perspective, design issues, website architecture and design quality of the tourism website. A population size of 400 respondents, which consist of both domestic and international tourist, are considered for the survey. Data is received from 360 respondents, which is analyzed using statistical tests like Cronbach’s alpha, Pearson’s correlation, cross-tabulations, bars charts and graphs to draw inferences and consclusion. The software-based test results serve as another important parameter for the evaluation of the current official website. This study brings out core needs of the tourist in terms of expectations from a tourism website and access technical quality of the current portal to provide necessary feedback and suggestions. The government officials, business houses, and web designers can utilize this work as a knowledge base to build tourism websites, which are user-centric. Further, the work is specifically important for Nepal government and tourism officials to identify shortcomings in their current website and make improvements for better design and user adaptability in future.
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