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Jeon, Che Ok,Park, Minjeong,Ro, Hyun-Su,Park, Woojun,Madsen, Eugene L. American Society for Microbiology 2006 Applied and environmental microbiology Vol.72 No.2
<B>ABSTRACT</B><P><I>Polaromonas naphthalenivorans</I> CJ2, found to be responsible for the degradation of naphthalene in situ at a coal tar waste-contaminated site (C.-O. Jeon et al., Proc. Natl. Acad. Sci. USA 100:13591-13596, 2003), is able to grow on mineral salts agar media with naphthalene as the sole carbon source. Beginning from a 484-bp <I>nagAc</I>-like region, we used a genome walking strategy to sequence genes encoding the entire naphthalene degradation pathway andadditional flanking regions. We found that the naphthalene catabolic genes in <I>P. naphthalenivorans</I> CJ2 were divided into one large and one small gene cluster, separated by an unknown distance. The large gene cluster (<I>nagRAaGHAbAcAdBFCQEDJI′ORF1tnpA</I>) is bounded by a LysR-type regulator (<I>nagR</I>). The small cluster (<I>nagR2ORF2I'KL</I>) is bounded by a MarR-type regulator (<I>nagR2</I>). The catabolic genes of <I>P. naphthalenivorans</I> CJ2 were homologous to many of those of <I>Ralstonia</I> U2, which uses the gentisate pathway to convert naphthalene to central metabolites. However, three open reading frames (<I>nagY</I>, <I>nagM</I>, and <I>nagN</I>), present in <I>Ralstonia</I> U2, were absent. Also, <I>P. naphthalenivorans</I> carries two copies of gentisate dioxygenase (<I>nagI</I>) with 77.4% DNA sequence identity to one another and 82% amino acid identity to their homologue in <I>Ralstonia</I> sp. strain U2. Investigation of the operons using reverse transcription PCR showed that each cluster was controlled independently by its respective promoter. Insertional inactivation and lacZ reporter assays showed that <I>nagR2</I> is a negative regulator and that expression of the small cluster is not induced by naphthalene, salicylate, or gentisate. Association of two putative <I>Azoarcus</I>-related transposases with the large cluster and one <I>Azoarcus</I>-related putative salicylate 5-hydroxylase gene (<I>ORF2</I>) in the small cluster suggests that mobile genetic elements were likely involved in creating the novel arrangement of catabolic and regulatory genes in <I>P. naphthalenivorans</I>.</P>
Anti-melanogenesis effect of 2,5-dimethyM-hydroxy-3 [2H]- furanone
( Che-ok Jeon ),( Ji-yeon Ohf ),( Jae-sook Koh ),( Sung-won Jung ),( Jung-yeon Kim ) 대한화장품학회 1996 대한화장품학회지 Vol.22 No.2
DMHF (2.5-dimethyl-4-hydroxy-3[2H]-furanone), an antioxidative compound from the reaction of L-cysteine/D-glucose scavenged efficiently 1,1-diphenyl-2-picryl hydrazyl free radicals. It exhibited an inhibitory effect on the autoxidation of linolenic acid, and the protective effect against UV cytotoxicity in cultured human fibroblast. In addition, DMHF appeared to prevent the cellular melanogenesis in the cultured murine melanoma cells more effectively than kojic acid, a well known inhibitor of melanogenesis, while the former was not so effective as the latter for the inhibition of the tyrosinase. Considering that cellular melanogenesis is a metabolic process triggered by oxidative stress, it ovas tentatively deduced that the antioxidative property of DMHF might afford the effect against cellular pigmentation by alleviating the causative stress. In toxicological tests such as irritation and sensitization, this compound turned out to be safe. The results of this study suggest that DMHF may be a novel inhibitor of melanogenesis, and that might be useful for application in cosmetics.
Jeon, Che-Ok,Lim, Jee-Min,Lee, Jae-Chan,Lee, Gye-Suk,Lee, Jung-Min,Xu, Li-Hua,Jiang, Cheng-Lin,Kim, Chang-Jin Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.
A Gram-positive, spore-forming and moderately halophilic bacterium, strain BH139^(T), was isolated from saline sediment of Xinjiand Province, China. Cells of strain BH139^(T) were motile, flagellated rods. The organism grew optimally at 30-35℃ in the presence 12-14% (w/v) NaCl. The major fatty acids were branched saturated fatty acids such as iso-C_(16:0), anteiso-C_(15:0), iso-C_(15:0), iso-C_(14:0) and anteiso-C_(17:0). The G+C content of the genomic DNA was about 43 mol% and the predominant isoprenoid quinone was MK-7. Comparative 16S rRNA gene sequence analyses showed that strain BH139^(T) was most closely related to Lentibacillus salicampi KCCM 41560^(T) (96·9% 16S rRNA gene sequence similarity) and formed a distinct phyletic line from that species. On the basis of physiological and molecular properties, the isolate represents a novel species within the genus Lentibacillus, for which the name Lentibacillus salarius sp. nov. is proposed. The type strain is BH139^(T) (=KCTC 3911^(T)=DSM 16459^(T)).
Jeon, Che-Ok,Lim, Jee-Min,Lee, Jung-Min,Xu, Li-Hua,Jiang, Cheng-Lin,Kim, Chang-Jin Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.
A spore-forming, halophilic bacterium, designated strain BH163^(T), was isolated from a salt lake in China. Cells were motile, strictly aerobic rods that contained type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid. The isolate showed Gram- and catalase-positive reactions and formed a terminal endospore with a swollen sporangium. The major cellular fatty acids were anteiso-C_(15:0), iso-C_(15:0), anteiso-C_(17:0) and iso-C_(16:0). The genomic DNA G+C content of the strain was 41·0 mol%. Comparative analysis of 16S rRNA gene sequences showed that strain BH163^(T) formed a distinct line within the phyletic group classically defined as the genus Bacillus and was most closely related to the taxa [Bacillus] haloalkaliphilus DSM 5271^(T) and Filobacillus milosensis DSM 13259^(T), with 16S rRNA gene sequence similarities of 95·9 and 94·5%, respectively. On the basis of physiological and molecular properties, it is proposed that [Bacillus] haloalkaliphilus DSM 5271^(T) is reclassified in the new genus Alkalibacillus as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. Strain BH163^(T) (=KCTC 3916^(T)=DSM 16460^(T)) was assigned as the type strain of the novel species Alkalibacillus salilacus.