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( Eunhee Lee ),( Kayeong Yun ),( Sieun Han ),( Bosun Joo ),( Kyusup Lee ) 대한산부인과학회 2018 대한산부인과학회 학술대회 Vol.104 No.-
Objective: The aim of this study was to suggest the most effective primary ovarian insufficiency(POI) model construction method by examining the effect of POI model on anticancer drugs. Methods: Female mice were treated with cyclophosphamide(Cy)+busulfan(Bu) mixture for 1 week to 4 weeks and Cisplatin alone for 3, 5, 10, and 14 days. The injection period to POI occurrence was observed and the period of normal recovery from POI was checked. Results: After two weeks of the Cy+Bu injection, the weight of ovary and uterus decreased sharply and the weight reductions also affect ovarian function. And ovarian function is not recovered for at least 2 weeks when POI is induced with Cy+BU for 2 weeks. After 10 days of Cisplatin injection, the weight of ovary and uterus decreased significantly and the weight reductions also affect ovarian function. After 10 to 2 weeks of treatment with cisplatin, the embryo development rate to blastocyst was 0%. Conclusion: As an ideal POI induction model, it is useful to treat Cy+Bu for 2 weeks or cisplatin for 10 days to 2 weeks.
( Hee-jung Choi ),( Mi-ju Park ),( Bo-sung Kim ),( Hee-jin Choi ),( Bosun Joo ),( Kyu Sup Lee ),( Jung-hye Choi ),( Tae-wook Chung ),( Ki-tae Ha ) 생화학분자생물학회(구 한국생화학분자생물학회) 2017 BMB Reports Vol.50 No.8
Endometriosis is the abnormal growth of endometrial cells outside the uterus, causing pelvic pain and infertility. Furthermore, adhesion of endometrial tissue fragments to pelvic mesothelium is required for the initial step of endometriosis formation outside uterus. TGF-β1 and adhesion molecules importantly function for adhesion of endometrial tissue fragments to mesothelium outside uterus. However, the function of TGF-β1 on the regulation of adhesion molecule expression for adhesion of endometrial tissue fragments to mesothelium has not been fully elucidated. Interestingly, transforming growth factor β1 (TGF-β1) expression was higher in endometriotic epithelial cells than in normal endometrial cells. The adhesion efficiency of endometriotic epithelial cells to mesothelial cells was also higher than that of normal endometrial cells. Moreover, TGF-β1 directly induced the adhesion of endometrial cells to mesothelial cells through the regulation of integrin of αV, α6, β1, and β4 via the activation of the TGF-β1/TGF-βRI/Smad2 signaling pathway. Conversely, the adhesion of TGF-β1-stimulated endometrial cells to mesothelial cells was clearly reduced following treatment with neutralizing antibodies against specific TGF-β1-mediated integrins αV, β1, and β4 on the endometrial cell membrane. Taken together, these results suggest that TGF-β1 may act to promote the initiation of endometriosis by enhancing integrin-mediated cell-cell adhesion. [BMB Reports 2017; 50(8): 429-434]