Hepatocarcinogenesis induced by diethylnitrosamine in B6C3F₁ mice

Kyoung Mo Yang,Seung Hwan Son,Tae Myoung Kim,Hyoung Seok Lee,Bong Su Park,Young Bo Shim,Dae Joong Kim 한국실험동물학회 2008 한국실험동물학회 학술발표대회 논문집 Vol.2008 No.-

Ulleungamides A and B, Modified α,β-Dehydropipecolic Acid Containing Cyclic Depsipeptides from <i>Streptomyces</i> sp. KCB13F003

Son, Sangkeun,Ko, Sung-Kyun,Jang, Mina,Lee, Jae Kyoung,Ryoo, In-Ja,Lee, Jung-Sook,Lee, Kyung Ho,Soung, Nak-Kyun,Oh, Hyuncheol,Hong, Young-Soo,Kim, Bo Yeon,Jang, Jae-Hyuk,Ahn, Jong Seog American Chemical Society 2015 ORGANIC LETTERS Vol.17 No.16

<P>Two novel cyclic depsipeptides, ulleungamides A (1) and B (2), were isolated from cultures of terrestrial Streptomyces sp. Their structures were determined by analyses of spectroscopic data and various chemical transformations, including modified Mosher's method, advanced Marfey's method, PGMF,, GITC derivatizations, and Snatzke's method. Ulleungamides were determined to be a new class of peptides bearing unprecedented units, such as 5-hydroxy-6-methyl-2,3-dehydropipecolic acid, 4,5-dihydroxy-6-methyl-2,3-dehydropipecolic acid, and amino-linked 2-isopropylsuccinic acid. Ulleungamide A displayed growth inhibitory activity against Staphylococcus aureus and Salmonella typhimurium without cytotoxicity.</P>

Polyketides and Anthranilic Acid Possessing 6-Deoxy-α-<small>l</small>-talopyranose from a <i>Streptomyces</i> Species

Son, Sangkeun,Ko, Sung-Kyun,Jang, Mina,Lee, Jae Kyoung,Kwon, Min Cheol,Kang, Dong Hyo,Ryoo, In-Ja,Lee, Jung-Sook,Hong, Young-Soo,Kim, Bo Yeon,Jang, Jae-Hyuk,Ahn, Jong Seog American Chemical Society and American Society of 2017 Journal of natural products Vol.80 No.5

<P>A bioassay-guided investigation in conjunction with chemical screening led to the isolation of three new glycosides, ulleungoside (1), 2-methylaminobenzoyl 6-deoxy-alpha-L-talopyranoside (2), and naphthomycinoside (3), along with three known secondary metabolites (5-7) from Streptomyces sp. KCB13F030. Their structures were elucidated by detailed NMR and MS spectroscopic analyses. Absolute configurational analysis of the sugar units based on the magnitudes of the coupling constants, NOESY correlations, chemical derivatization, and optical rotation measurements revealed that compounds 1-3 and 5 incorporate the rare deoxyhexose 6-deoxy-alpha-L-talopyranose. The absolute configuration of a polyketide extender unit of 3 was determined by applying the J-based configuration analysis and modified Mosher's method. Ulleungoside (1) and naphthomycin A (7) showed activity. Further bioevaluation revealed that compounds 1 and cell lines, and compounds 5 and 6, which are members of the 2 plying the J-based configuration analysis and modified Mosher's in vitro inhibitory effects against indoleamine 2,3-dioxygenase 7 had moderate antiproliferative activities against several cancer piericidin family, induced autophagosome accumulation.</P>

Development of Horticultural Therapy Assessment Tool for Dementia

Moon-Kyoung Cho,Eun-Ah Lee,Bo-Young Kim,Ki-Cheol Son 한국원예학회 2015 한국원예학회 학술발표요지 Vol.2015 No.5

국내에서 식물공장용 배지 재료로 유통되는 피트모스와 코이어 더스트의 물리·화학적 특성

신보경(Bo Kyoung Shin),손정익(Jung Eek Son),최종명(Jong Myung Choi) (사)한국생물환경조절학회 2012 생물환경조절학회지 Vol.21 No.4

유기 배지재료로써 국내에서 유통되는 피트모스와 코이어 더스트의 물리·화학적 특성을 분석하여 식물공장용 혼합배지 조제를 위한 기초자료를 얻기 위하여 본 연구를 수행하였다. 연구목적을 달성하기 위해 캐나다, 리투아니아, 라트비아로부터 수입되는 피트모스 6점과 인도, 스리랑카, 베트남으로부터 수입되는 코이어 더스트 10점을 확보한 후 물리적 특성과 화학적 특성을 분석하고 비교하였다. 공극률에서 피트모스가 평균 ±표준편차가 79.6 ± 5.04%로써 83.6 ± 6.18인 코이어 더스트 보다 낮았으며, 용기용수량은 피트모스가 평균 69.9 ± 10.17%이고 코이어 더스트는 65.9 ± 3.46으로 피트모스의 보수성이 코이어 더스트 보다 높았지만 종류별 차이가 컸다. 기상률은 4종류의 피트모스가 10% 이하로 측정되었지만 코이어 더스트는 12~26% 의 범위로 피트모스보다 월등히 높았다. 조사한 6종류의 피트모스는 쉽게 흡수할 수 있는 수분(easily available water, EAW)이 18~22%의 범위였던 반면 코이어 더스트는 11~16%로 측정되었고 1점을 제외한 9점이 11~13%의 범위로 측정되어 매우 낮았다. 완충수(buffering water)도 피트모스가 9~13%의 범위였지만 9점의 코이어 더스트가 5.5~7.5%로 측정되어 정확한 물 관리가 필요함을 나타내고 있다. 화학적 특성에서 피트모스는 pH가 3.46~4.17인 강산성물질이었고, 전기전도도(EC)는 분석한 6점 중 5점이 0.137~0.254dS · m<SUP>?1</SUP>로 측정되었다. 코이어 더스트의 pH는 5.31~6.48인 약산성 물질이었으며, EC는 평균±표준편차가 0.563 ± 0.83dS · m<SUP>?1</SUP>로 피트모스 보다 월등히 높고 종류 간 차이가 큼을 나타내고 있다. 양이온교환용량(CEC)은 코이어 더스트가 피트모스보다 약 3~4배 가량 낮았다. 코이어 더스트가 피트모스 보다 NO3-N와 P2O5-P의 함량이 높았고, NH4-N 함량이 낮았지만 K와 Na 함량은 월등히 높아 전기전도도가 높아진 원인이 되었다고 판단하였다. 피트모스와 코이어 더스트의 온수추출물은 각각 6.67~16.37% 및 30.0~65.1%, 알칼리 추출물은 0~38% 및 23.1~70.3%였고 생장억제물질 함량이 코이어 더스트에서 높을 수 있음을 의미하고 있다. Objective of this research was to secure the information on physical and chemical properties of peatmoss and coir dust. To achieve this, 6 kinds of peatmoss and 10 kinds of coir dust currently used in the country as the root medium components in plant factories were collected and analysed. The mean ± standard deviation (SD) of total porosity and container capacity in peatmoss and coir dust were 79.6 ± 5.04 and 83.6 ± 6.18%, and 69.9 ± 10.17 and 65.9 ± 3.46%, respectively. These indicate that peatmoss has higher water holding capacity than coir dust and the characteristics are highly varied among peatmoss. The 4 out of 5 kinds of peatmoss had lower than 10%, but coir dust had 12~26%, of air-filled porosity. The percentage of easily available water and buffering water in peatmoss and coir dust was 18~22 and 11~16% and 9~13 and 5.5~7.5%, respectively. These results indicate that precise irrigation is required when coir dust is used as the root medium. The ranges of pH and electrical conductivity (EC) were 3.46~4.17 and 0.137~0.253 dS · m<SUP>?1</SUP> in peatmoss and 5.31~6.48 and 0.250~0.1.580 dS · m<SUP>?</SUP>1 in coir dust. However, 0.563 ± 0.83 dS · m<SUP>?1</SUP> in mean ± SD of coir dust EC indicates that it is higher than that of peatmoss, and the coir dust are highly varied in EC. The cation exchange capacity of peatmoss was 3 to 4 times as high as that of coir dust. The coir dust had higher NO3 and PO4 and lower NH4 than peatmoss. The K and Na concentrations in coir dust were extremely high indicating that these ions caused the rising in EC. The percentage of hot water and alkali extracts of peatmoss were 6.67~16.37 and 0~38%, whereas those of coir dust were 30.0~65.1 and 23.1~70.3%. These results mean that possible existence of growth inhibiting materials in coir dust.

국내에서 식물공장용 배지 재료로 유통되는 무기물의 토양 물리화학적 특성

신보경(Bo Kyoung Shin),손정익(Jung Eek Son),최종명(Jong Myung Choi) (사)한국생물환경조절학회 2012 생물환경조절학회지 Vol.21 No.4

국내에서 유통되고 있는 무기배지재료인 버미큘라이트(12점)와 펄라이트(5점)의 물리·화학적 특성을 분석하여 혼합배지 조제를 위한 기초자료를 얻기 위하여 본 연구를 수행하였다. 710㎛ 이상의 직경을 갖는 입자의 비율이 버미큘라이트 중 중국산 silver 3~8㎜가 99.7%로 조사되어 가장 비율이 높았고, 펄라이트는 3~5㎜에서 99.9%로 가장 높았다. 펄라이트(< 1㎜)와 남아프리카공화국산 silver 버미큘라이트(0.25~0.7㎜)의 용기용수량은 각각 72.0 및 71.1%로 가장 높았고, 기상률은 중국산 silver 3~8㎜가 49.3%로 뚜렷하게 높았다. 펄라이트 5종류의 공극률은 60% 이상으로 측정되었으며, 용기용수량은 1㎜ 이하의 규격을 갖는 물질을 제외하고 20.4~39.7%의 범위에 포함되었다. 버미큘라이트 중 중국산 gold 0.3~1㎜와 남아프리카공화국산 0.25~0.7㎜가 쉽게 이용할 수 있는 수분(EAW)와 완충수(BW)의 비율이 높았고, 펄라이트는 1㎜ 이하와 0.7~1.5㎜의 EAW와 BW의비율이 높았다. 화학적 특성에서 버미큘라이트는 pH가 6.36~10.74 범위에 포함되는강알칼리성 물질이었고, 전기전도도(EC)는 0.032~0.393dS · m<SUP>?1</SUP>로 측정되어 종류간 차이가 큼을 알 수 있었다. 펄라이트의 pH는 7.788.62 범위에 포함되어 약알칼리성이었으며, EC는 0.030~0.041dS · m<SUP>?1</SUP>로측정되었다. 양이온교환용량은중 국산 silver 버미큘라이트 0.3~1㎜가 14.7c㏖+· ㎏<SUP>?1</SUP>으로 가장 높았고, 펄라이트는 0.71.5㎜가 1.51c㏖+ · ㎏<SUP>?1</SUP>로 가장 높았는데 버미큘라이트보다 약 10배 가량 낮았다. 버미큘라이트는 펄라이트보다 Ca, K 및 Na과 같은 치환성 양이온 함량이 많았다. Inorganic materials were co㎜only used as container media in domestic plant factories. Objective of this research was to secure the information in soil physical and chemical properties of inorganic materials such as vermiculites and perlites. To achieve this, 12 gold and silver vermiculites from China, Zimbabwe, and South Africa and 5 perlites from China were collected based on the marketing grades (MG) in particle sizes and analyzed for determination of their characteristics. The percentage of particles larger than 710 ㎛, in China perlite MG 3~5 ㎜, China silver vermiculites MG > 8 ㎜ and MG 3~8 ㎜ were 99.9%, 99.8%, and 99.7%, respectively, which were much higher than 28.4% in China gold vermiculite MG 0.3~1.0 ㎜, 14.0% in perlite MG < 1.0 ㎜, and 12.6% of Zimbabwe silver vermiculite MG < 1.0 ㎜. The container capacities of perlite MG < 1.0 ㎜ and South Africa silver vermiculite MG 0.25~1.0 ㎜ were 72.0% and 71.1%, respectively. The air space in China silver vermiculite MG 3~8 ㎜ was 49.3% which was higher than other materials tested. However, the China gold and silver vermiculites MG 0.3~1 ㎜ had 3.5% and 2.4% in air space indicating that possible problems could occur in soil aeration when they are used for container media. The percentage of easily available and buffering water of China gold vermiculite MG 0.3~1 ㎜ and perlite MG < 1.0 ㎜ were the highest among test materials. The ranges of pH and electrical conductivity were 6.36 to 10.7 and 0.032 to 0.393 dS · m<SUP>?1</SUP> in vermiculites and 7.78 to 8.62 and 0.030 to 0.041 dS · m<SUP>?1</SUP> in perlite, respectively. The cation exchange capacity of China silver vermiculite MG 0.3~1 ㎜ were 14.7 c㏖ · ㎏<SUP>?1</SUP> that was 10 times as high as 0.34 c㏖ · ㎏<SUP>?1</SUP> in perlite MG 1~2.5 ㎜. The vermiculites had the higher contents of exchangeable cations such as Ca, K, and Na, than those of perlites.

비료종류 및 농도가 상추의 생장과 근권 환경 변화에 미치는 영향

신보경(Bo Kyoung Shin),손정익(Jung Eek Son),최종명(Jong Myung Choi) 한국원예학회 2012 한국원예학회 학술발표요지 Vol.2012 No.10

딥러닝을 적용한 주간 전력수요예측에 대한 기상인자 선정

권보성(Bo-Sung Kwon),문찬호(Chan-Ho Moon),송경빈(Kyung-Bin Song),심현보(Hyun-Bo Shim),신기준(Ki-Jun Shin),손흥구(Heung-Gu Son) 한국조명·전기설비학회 2022 한국조명·전기설비학회 학술대회논문집 Vol.2022 No.5

Shape-coded silica nanotubes for multiplexed bioassay: rapid and reliable magnetic decoding protocols.

He, Bo,Kim, Sung Kyoung,Son, Sang Jun,Lee, Sang Bok Future Medicine Ltd 2010 Nanomedicine Vol.5 No.1

<P>Aims: The recent development of 1D barcode arrays has proved their capabilities to be applicable to highly multiplexed bioassays. This article introduces two magnetic decoding protocols for suspension arrays of shape-coded silica nanotubes to process multiplexed assays rapidly and easily, which will benefit the minimization and automation of the arrays. Methods: In the first protocol, the magnetic nanocrystals are incorporated into the inner voids of barcoded silica nanotubes in order to give the nanotubes magnetic properties. The second protocol is performed by trapping the barcoded silica nanotubes onto streptavidin-modified magnetic beads. Results: The rapid and easy decoding process was demonstrated by applying the above two protocols to multiplexed assays, resulting in high selectivity. Furthermore, the magnetic bead-trapped barcode nanotubes provided a great opportunity to exclude the use of dye molecules in multiplexed assays by using barcode nanotubes as signals. Conclusion: The rapid and easy manipulation of encoded carriers using magnetic properties could be used to develop promising suspension arrays for portable bioassays.</P>

천문동 건근의 항산화 효과에 관한 연구

구경윤(Kyoung Yoon Koo),김원백(Won Baek Kim),박소해(So Hae Park),김민지(Minji Kim),김보람(Bo Ram Kim),황지회(Jihoe Hwang),김민정(Min Jung Kim),손홍주(Hong Joo Son),황대연(Dae Youn Hwang),김동섭(Dong Seob Kim),이충열(Chung Yeoul Lee) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.4

본 연구에서는 천문동 건근의 부위에 따른 항산화 활성을 비교하여 이와 관련된 물질을 탐색하고 동정하였다. 천문동 건근에서 항산화 활성이 높은 부위는 껍질에 해당하고 DPPH radical 소거능, nitrite 소거능, hydroxy radical 소거능 및 ABTS 양이온 소거능과 같은 항산화 활성이 껍질 부위에서 상대적으로 높은 것을 확인할 수 있었으며, 이러한 결과는 껍질에 존재하는 폴리페놀 화합물에 의한 것으로 판단되었다. 껍질에 존재하는 항산화 활성 물질을 HPLC를 이용하여 분리하고 동정한 결과 리그닌의 생합성에 중요한 전구물질인 caffeic acid가 주요 항산화 활성 물질이었으며, 이러한 결과를 토대로 천문동이 지닌 효능을 최대로 사용하기 위해서는 천문동의 건조 후에 껍질을 제거하지 않고 사용하는 것이 바람직한 것으로 생각한다. This study was performed to compare the antioxidative activities of methanol extracts from Asparagus cochinchinensis with whole root (W-AC), flesh (F-AC), and root bark (B-AC). To evaluate the antioxidative properties of their methanol extracts, 1,1-diphenyl-2-picrylhydrazyl radical, nitrite, hydroxyl radical, 2,2"-azino-bis(3-ethylbenz thiazoline-6-sulfonate) radical scavenging activities, and contents of total flavonoid and polyphenol contents were measured. B-AC extract showed the highest antioxidative activity, whereas F-AC extract showed the lowest. For B-AC extract, caffeic acid was isolated by preparative high-performance liquid chromatography and confirmed by liquid chromatography-mass spectrometry and absorption spectroscopy, which showed 1.6% of total polyphenol contents among all methanol extracts.