장기간의 태권도 수련이 혈액 항산화 효소 및 지질과산화에 미치는 영향 = Effects of TaeKwonDo training on blood antioxidant enzymes and lipid peroxidation

다국어 초록 (Multilingual Abstract)

The generation of reactive oxygen species(ROS) in biological tissues is dependant on the consumption of oxygen. Exercise seems to induce the ROS generation by increased oxygen consumption. The increased production of ROS during exercise can exacerbate oxidative tissue injury if these species are not scavenged by antioxidant defence systems. The antioxidant defence systems in human body comprised of antioxidant enzymes; superoxide dismutase(SOD), catalase(CAT) and glutathione peroxidase(GPX), and antioxidant substance; glutathione, antioxidant vitamins and several proteins. A recent review showed that physical exercise enhanced antioxidant enzymes activity and altered reduced glutathione(GSH) levels in various organs. However, changing pattern of antioxidant enzymes activities and glutathione levels by physical exercise were not well defined. The aim of this study is to evaluation of the influence of TaeKwonDo training on oxidative stress by determinations of antioxidant systems and lipid peroxidation level in blood of human subject. In this study, two groups consisting of 10 male subjects who are TaeKwonDo training for over 6 years and 10 normal male subjects ages 20 to 24 years. The blood samples were taken from the cubital vein of all 20 experimental human subjects they are stopped physical training for 12 hrs. The activity of superoxide dismutase(SOD), glutathione peroxidase(GSHPX), and catalase(CAT) were determined in erythrocytes. Also, erythrocyte thiobarbituric acid-reactive substances(TBARS) levels as a marker of lipid peroxidation was measured. The glutathione(GSH) concentration was also measured in whole blood.
A statistically significant decrease of SOD activity in erythrocytes of TaeKwonDo training group was found(P＜0.01). The CAT activity and TBAR concentration in erythrocytes increased in a statistically significant way in TaeKwonDo training group compared with normal control group(P＜0.01). A statistically significant decrease of glutathione concentration in whole blood was observed in TaeKwonDo training group(P＜0.01). These data show that erythrocytes oxidative stress increased in TaeKwonDo training which may due to increasing generation of ROS during training exercise.

목차 (Table of Contents)

• 목차 = ⅰ
• ABSTRACTS = ⅵ
• Ⅰ. 서론 = 1
• 1. 연구의 필요성 = 1
• 2. 연구의 목적 = 4
• 3. 연구가설 = 5
• 4. 연구의 제한점 = 5
• 5. 용어의 정의 = 6
• Ⅱ. 이론적 배경 = 8
• 1. 태권도의 에너지 대사적 측면 = 8
• 2. 활성산소 = 13
• 3. 항산화제 = 16
• 4. 운동과 항산화 방어 (Action of antioxidant defence) = 18
• 5. 운동과 항산화작용 = 20
• 6. 항산화제의 효과 = 40
• 7. 혈액의 구성 = 48
• Ⅲ. 연구방법 = 54
• 1. 연구 대상 = 54
• 2. 시료의 조제 = 55
• 3. 실험설계 = 55
• 4. Superoxide dismutase 활성도 측정 = 57
• 1) Warking solution = 57
• 2) 방법 = 57
• 5. Catalse 활성도 측정 = 58
• 1) Walking solution = 58
• 2) 방법 = 58
• 6. Glutathione peroxidase 활성도 측정 = 59
• 1) Warking solution = 59
• 2) 방법 = 59
• 7. Thiobarbituric acid 반응성 물질의 측정 = 60
• 1) Walking solution = 60
• 2) 방법 = 60
• 8. 혈청 알부민, sGOT, sGPT및 alkaline phosphatase 측정 = 61
• 9. 혈액 적혈구, 백혈구, 혈소판, 헤모글로빈량 측정 = 61
• 10. 통계처리 방법 = 61
• Ⅳ. 연구 결과 = 62
• 1. 태권도 수련후의 항산화계 변화 = 62
• 1) 태권도선수의 적혈구 SOD 활성도 = 62
• 2) 태권도선수의 적혈구 Catalase 활성도 = 63
• 3) 태권도선수의 적혈구 GSHPx 활성도 = 65
• 4) 태권도선수의 적혈구 TBARS 량 = 66
• 2. 태권도선수의 혈액성분 검사 = 67
• 1) 태권도선수의 혈청 효소 활성도 = 67
• 2) 태권도선수의 혈액성분 검사 = 68
• 3) 태권도선수의 혈액 glutathione량 = 69
• Ⅴ. 논의 = 71
• 1. 태권도 수련이 항산화계에 미치는 영향 = 71
• 2. 태권도선수의 혈액성분에 미치는 영향 = 75
• Ⅵ. 결론 및 제언 = 78
• 1. 결론 = 78
• 2. 제언 = 79
• 참고문헌 = 80