何首烏가 고지혈증 흰쥐의 허혈성 뇌손상에 미치는 영향 = Effects of Polygoni Multiflori Radix on cerebral ischemia of hyperlipidemic rats

다국어 초록 (Multilingual Abstract)

This study investigated neuroprotective effects of Polygoni Multiflori Radix on cerebral ischemia of hyperlipidemic rats. Effects of Polygoni Multiflori Radix were evaluated with changes of infarct size after the focal cerebral ischemia induced by the middle cerebral artery occlusion, changes of pyramidal neurons and expressions of Bax and Bcl-2, apoptosis regulating factors, after the global cerebral ischemia, and changes of serum lipid revels after the cerebral ischemia.
Results obtained are as follows;
1. Polygoni Multiflori Radix did not reduce the focal cerebral infarct size induced by the middle cerebral artery occlusion under both of hyperlipidemic and normal-lipid conditions.
2. Polygoni Multiflori Radix significantly reduced the increase of neuronal cell death in CA1 region of hippocampus induced by the global cerebral ischemia under both of hyperlipidemic and normal-lipid conditions.
3. Polygoni Multiflori Radix significantly reduced the increase of Bax expression in CA1 region of hippocampus induced by global cerebral ischemia under both of hyperlipidemic and normal-lipid conditions.
4. Polygoni Multiflori Radix significantly increased Bcl-2 expression in CA1 region of hippocampus after global cerebral ischemia under normal-lipid condition, but was not effective on that under hyperlipidemic condition.
5. Polygoni Multiflori Radix was not effective on serum total-cholesterol, HDL-cholesterol, LDL-cholesterol and triglyceride levels under normal-lipid condition, irrespective of the focal cerebral infarct and the global cerebral ischemia.
6. Polygoni Multiflori Radix significantly reduced the increase of serum total-cholesterol and triglyceride levels, and increased serum LDL-cholesterol level under hyperipidemic condition, irrespective of the focal cerebral infarct and the global cerebral ischemia.

목차 (Table of Contents)

• 目次
• I. 서론 = 1
• II. 재료 및 방법 = 3
• 1. 재료 = 3
• 1) 약물의 조제 및 투여 = 3
• 2) 동물 = 3
• 2. 방법 = 3
• 1) 고지혈증 유발 = 3
• 2) 국소 뇌경색에 대한 실험 = 4
• (1) 국소 뇌경색 유발 = 4
• (2) TTC 염색 및 국소 뇌경색 면적의 측정 = 6
• 3) 전뇌허혈에 대한 실험 = 6
• (1) 전뇌허혈 유발 = 7
• (2) 뇌조직 처리 = 8
• (3) Cresyl violet 염색 및 전뇌허혈부위의 신경세포수와 신경세포층 두께측정 = 8
• (4) Bax와 Bcl-2의 면역조직화학염색 변화 관찰 = 8
• 4) 혈청 지질함량의 측정 = 9
• 3. 통계처리 = 10
• III. 실험성적 = 11
• 1. 국소 뇌경색에 대한 실험 = 11
• 1) 국소 뇌경색 면적에 미치는 영향 = 11
• 2) 국소 뇌경색 체적에 미치는 영향 = 14
• 2. 전뇌허혈에 대한 실험 = 18
• 1) 전뇌허혈 뇌해마의 신경세포에 미치는 영향 = 18
• 2) 뇌해마의 Bax 및 Bcl-2 발현에 미치는 영향 = 22
• 3. 혈청 total-cholesterol에 미치는 영향 = 26
• 4. 혈청 HDL-cholesterol에 미치는 영향 = 29
• 5. 혈청 LDL-cholesterol에 미치는 영향 = 32
• 6. 혈청 triglyceride에 미치는 영향 = 35
• IV. 고찰 = 38
• V. 결론 = 43
• VI. 참고문헌 = 45
• ABSTRACT = 53