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Assessment of a HER2 scoring system for gastric cancer: results from a validation study
Hofmann, M,Stoss, O,Shi, D,Bü,ttner, R,van de Vijver, M,Kim, W,Ochiai, A,Rü,schoff, J,Henkel, T Blackwell Publishing Ltd 2008 Histopathology Vol.52 No.7
<P>Aims: </P><P>Human epidermal growth factor receptor 2 (HER2) overexpression/amplification is implicated in the development of various solid tumour types. Validated methods and scoring systems for evaluating HER2 status exist in breast cancer, but not in gastric cancer. The aim was to establish a HER2 scoring system for gastric cancer to identify suitable patients for enrolment in a trial of trastuzumab (Herceptin<SUP>®</SUP>) in advanced metastatic gastric cancer.</P><P>Methods and results: </P><P>Formalin-fixed paraffin-embedded gastric cancer samples were tested for HER2 status using the fluorescence <I>in situ</I> hybridization (FISH) pharmDx™ kit (Dako Denmark A/S). Immunohistochemistry (IHC) was performed using the HercepTest™ (Dako). Concordance between FISH and IHC was 93.5% in 168 evaluable samples. Eleven samples were scored as FISH+ but IHC− or equivocal.</P><P>Conclusions: </P><P>IHC/FISH discrepancies were attributed to basolateral membranous immunoreactivity of glandular cells resulting in incomplete membranous reactivity and/or a higher rate of tumour heterogeneity in gastric cancer compared with breast cancer. With modifications to the IHC scoring system, the HercepTest™ is considered valid for the identification of HER2+ gastric tumours for this clinical trial. Correlation of HER2 scores with clinical outcomes will be needed to determine which patients might benefit from trastuzumab therapy.</P>
New results on ν μ → ν τ appearance with the OPERA experiment in the CNGS beam
Agafonova, N.,Aleksandrov, A.,Anokhina, A.,Aoki, S.,Ariga, A.,Ariga, T.,Asada, T.,Autiero, D.,Badertscher, A.,Ben Dhahbi, A.,Bender, D.,Bertolin, A.,Bozza, C.,Brugnera, R.,Brunetti, G.,Bü,ttner, B Springer-Verlag 2013 Journal of high energy physics Vol.2013 No.11
A BIBLIOMETRIC STUDY OF FEMALE PLUS SIZE CONSUMER CHALLENGE IN THE USA AND BRAZIL
Ana Julia Büttner,Marcelo Augusto Linardi,Suzane Strehlau 글로벌지식마케팅경영학회 2019 Global Fashion Management Conference Vol.2019 No.07
In Brazil overwieght people represent 58,2%, in the USA 40% of women’s population. Plus size is a standard of USA fashion industry that applies to woman who wears more than 12, and in Brazil it begins on 44. They are frustrated, because retails provide few options and don’t understand them.
Nghi Do Huu,Kellner Harald,Büttner Enrico,Huong Le Mai,Duy Le Xuan,Giap Vu Dinh,Quynh Dang Thu,Hang Tran Thi Nhu,Verberckmoes An,Diels Ludo,Liers Christiane,Hofrichter Martin 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.5
From the biotechnological viewpoint, the enzymatic disintegration of plant lignocellulosic biomass is a promising goal since it would deliver fermentable sugars for the chemical sector. Cellobiose dehydrogenase (CDH) is a vital component of the extracellular lignocellulose-degrading enzyme system of fungi and has a great potential to improve catalyst efficiency for biomass processing. In the present study, a CDH from a newly isolated strain of the agaricomycete Coprinellus aureogranulatus (CauCDH) was successfully purified with a specific activity of 28.9 U mg− 1. This pure enzyme (MW = 109 kDa, pI = 5.4) displayed the high oxidative activity towards β-1–4-linked oligosaccharides. Not least, CauCDH was used for the enzymatic degradation of rice straw without chemical pretreatment. As main metabolites, glucose (up to 165.18 ± 3.19 mg g− 1), xylose (64.21 ± 1.22 mg g− 1), and gluconic acid (5.17 ± 0.13 mg g− 1) could be identified during the synergistic conversion of this raw material with the fungal hydrolases (e.g., esterase, cellulase, and xylanase) and further optimization by using an RSM statistical approach.
CAR links hypoxia signaling to improved survival after myocardial infarction
Freiberg Fabian,Thakkar Meghna,Hamann Wiebke,Jacobo Lopez Carballo,Jüttner Rene,Voss Felizia K.,Becher Peter M.,Westermann Dirk,Tschöpe Carsten,Heuser Arnd,Rocks Oliver,Robert Fischer,Gotthardt Michae 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
The coxsackievirus and adenovirus receptor (CAR) mediates homo- and heterotopic interactions between neighboring cardiomyocytes at the intercalated disc. CAR is upregulated in the hypoxic areas surrounding myocardial infarction (MI). To elucidate whether CAR contributes to hypoxia signaling and MI pathology, we used a gain- and loss-of-function approach in transfected HEK293 cells, H9c2 cardiomyocytes and CAR knockout mice. CAR overexpression increased RhoA activity, HIF-1α expression and cell death in response to chemical and physical hypoxia. In vivo, we subjected cardiomyocyte-specific CAR knockout (KO) and wild-type mice (WT) to coronary artery ligation. Survival was drastically improved in KO mice with largely preserved cardiac function as determined by echocardiography. Histological analysis revealed a less fibrotic, more compact lesion. Thirty days after MI, there was no compensatory hypertrophy or reduced cardiac output in hearts from CAR KO mice, in contrast to control mice with increased heart weight and reduced ejection fraction as signs of the underlying pathology. Based on these findings, we suggest CAR as a therapeutic target for the improved future treatment or prevention of myocardial infarction.
Phospholipid dynamics in ex vivo lung cancer and normal lung explants
Lesko Julia,Triebl Alexander,Stacher-Priehse Elvira,Fink-Neuböck Nicole,Lindenmann Jörg,Smolle-Jüttner Freyja-Maria,Köfeler Harald C.,Hrzenjak Andelko,Olschewski Horst,Leithner Katharina 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
In cancer cells, metabolic pathways are reprogrammed to promote cell proliferation and growth. While the rewiring of central biosynthetic pathways is being extensively studied, the dynamics of phospholipids in cancer cells are still poorly understood. In our study, we sought to evaluate de novo biosynthesis of glycerophospholipids (GPLs) in ex vivo lung cancer explants and corresponding normal lung tissue from six patients by utilizing a stable isotopic labeling approach. Incorporation of fully 13 C-labeled glucose into the backbone of phosphatidylethanolamine (PE), phosphatidylcholine (PC), and phosphatidylinositol (PI) was analyzed by liquid chromatography/mass spectrometry. Lung cancer tissue showed significantly elevated isotopic enrichment within the glycerol backbone of PE, normalized to its incorporation into PI, compared to that in normal lung tissue; however, the size of the PE pool normalized to the size of the PI pool was smaller in tumor tissue. These findings indicate enhanced PE turnover in lung cancer tissue. Elevated biosynthesis of PE in lung cancer tissue was supported by enhanced expression of the PE biosynthesis genes ETNK2 and EPT1 and decreased expression of the PC and PI biosynthesis genes CHPT1 and CDS2 , respectively, in different subtypes of lung cancer in publicly available datasets. Our study demonstrates that incorporation of glucose-derived carbons into the glycerol backbone of GPLs can be monitored to study phospholipid dynamics in tumor explants and shows that PE turnover is elevated in lung cancer tissue compared to normal lung tissue.