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Cloning of Agarase Gene from Non-Marine Agarolytic Bacterium Cellvibrio sp
( Ariga Osamu ),( Takayoshi Inoue ),( Hajime Kubo ),( Kimi Minami ),( Mitsuteru Nakamura ),( Michi Iwai ),( Hironori Moriyama ),( Mitsunori Yanagisawa ),( Kiyohiko Nakasaki ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.9
Agarase genes of non-marine agarolytic bacterium Cellvibrio sp. were cloned into Escherichia coli and one of the genes obtained using HindIII was sequenced. From nucleotide and putative amino acid sequences (713 aa, molecular mass; 78,771 Da) of the gene, designated as agarase AgaA, the gene was found to have closest homology to the Saccharophagus degradans (formerly, Microbulbifer degradans) 2-40 aga86 gene, belonging to glycoside hydrolase family 86 (GH86). The putative protein appears to be a non-secreted protein because of the absence of a signal sequence. The recombinant protein was purified with anion exchange and gel filtration columns after ammonium sulfate precipitation and the molecular mass (79 kDa) determined by SDS-PAGE and subsequent enzymography agreed with the estimated value, suggesting that the enzyme is monomeric. The optimal pH and temperature for enzymatic hydrolysis of agarose were 6.5 and 42.5ºC, and the enzyme was stable under 40ºC. LC-MS and NMR analyses revealed production of a neoagarobiose and a neoagarotetraose with a small amount of a neoagarohexaose during hydrolysis of agarose, indicating that the enzyme is a β-agarase.
( Osamu Ariga ),( Naoki Okamoto ),( Naomi Harimoto ),( Kiyohiko Nakasaki ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.1
α-Neoagarooligosaccharide (α-NAOS) hydrolase was purified from Cellvibrio sp. OA-2007 by using chromatographic techniques after hydroxyapatite adsorption. The molecular masses of α-NAOS hydrolase estimated using SDS-PAGE and gel filtration chromatography were 40 and 93 kDa, respectively, and the optimal temperature and pH for the enzyme activity were 32ºC and 7.0-7.2. α-NAOS hydrolase lost 43% of its original activity when incubated at 35ºC for 30 min. The enzyme hydrolyzed neoagarobiose, neoagarotetraose, and neoagarohexaose to galactose, agarotriose, and agaropentaose, respectively, and produced 3,6-anhydro-L-galactose concomitantly; however, it did not degrade agarose.
New results on ν μ → ν τ appearance with the OPERA experiment in the CNGS beam
Agafonova, N.,Aleksandrov, A.,Anokhina, A.,Aoki, S.,Ariga, A.,Ariga, T.,Asada, T.,Autiero, D.,Badertscher, A.,Ben Dhahbi, A.,Bender, D.,Bertolin, A.,Bozza, C.,Brugnera, R.,Brunetti, G.,Bü,ttner, B Springer-Verlag 2013 Journal of high energy physics Vol.2013 No.11
Observation of a first ν<sub>τ</sub> candidate event in the OPERA experiment in the CNGS beam
Agafonova, N.,Aleksandrov, A.,Altinok, O.,Ambrosio, M.,Anokhina, A.,Aoki, S.,Ariga, A.,Ariga, T.,Autiero, D.,Badertscher, A.,Bagulya, A.,Bendhabi, A.,Bertolin, A.,Besnier, M.,Bick, D.,Boyarkin, V.,Boz North-Holland Pub. Co 2010 Physics letters: B Vol.691 No.3
The OPERA neutrino detector in the underground Gran Sasso Laboratory (LNGS) has been designed to perform the first detection of neutrino oscillations in direct appearance mode through the study of the ν<SUB>μ</SUB>->ν<SUB>τ</SUB> channel. The hybrid apparatus consists of an emulsion/lead target complemented by electronic detectors and it is placed in the high energy long-baseline CERN to LNGS beam (CNGS) 730 km away from the neutrino source. Runs with CNGS neutrinos were successfully carried out in 2008 and 2009. After a brief description of the beam, the experimental setup and the procedures used for the analysis of the neutrino events, we describe the topology and kinematics of a first candidate ν<SUB>τ</SUB> charged-current event satisfying the kinematical selection criteria. The background calculations and their cross-check are explained in detail and the significance of the event is assessed.
Final results of the search for νμ → νe oscillations with the OPERA detector in the CNGS beam
Agafonova, N.,Aleksandrov, A.,Anokhina, A.,Aoki, S.,Ariga, A.,Ariga, T.,Bertolin, A.,Bozza, C.,Brugnera, R.,Buonaura, A.,Buontempo, S.,Chernyavskiy, M.,Chukanov, A.,Consiglio, L.,D’Ambrosio, N.,De Lel Institute of Physics Pub 2018 The journal of high energy physics Vol.2018 No.6
Evidence forνμ→ντappearance in the CNGS neutrino beam with the OPERA experiment
Agafonova, N.,Aleksandrov, A.,Anokhina, A.,Aoki, S.,Ariga, A.,Ariga, T.,Asada, T.,Autiero, D.,Ben Dhahbi, A.,Badertscher, A.,Bender, D.,Bertolin, A.,Bozza, C.,Brugnera, R.,Brunet, F.,Brunetti, G.,Buon American Physical Society 2014 PHYSICAL REVIEW D - Vol.89 No.5